A5061802621- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Metalloenzymes and iron-sulfur proteins
- Mitochondrial Function and Pathology
- Microbial Fuel Cells and Bioremediation
- Anaerobic Digestion and Biogas Production
- Enzyme Structure and Function
- Microbial metabolism and enzyme function
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Pediatric Hepatobiliary Diseases and Treatments
- Methane Hydrates and Related Phenomena
- Ion Transport and Channel Regulation
- Porphyrin Metabolism and Disorders
- Carbon Dioxide Capture Technologies
- Electrocatalysts for Energy Conversion
- Vibrio bacteria research studies
- RNA modifications and cancer
Goethe University Frankfurt
2016-2025
Ourofino Saúde Animal (Brazil)
2025
University Hospital Münster
2023-2025
Anhanguera (Brazil)
2025
Goethe Institute
2008-2024
Ministry of Commerce of the People's Republic of China
2023
FEV (Germany)
2022-2023
Karlsruhe University of Education
2023
Max Rubner Institut
2023
BioEnergetics (United States)
2022
Storage and transportation of hydrogen is a major obstacle for its use as fuel. An increasingly considered alternative the direct handling to carbon dioxide (CO2) an intermediate storage material. However, CO2 thermodynamically stable, developed chemical catalysts often require high temperatures, pressures, and/or additives catalytic rates. Here, we present discovery bacterial hydrogen-dependent reductase from Acetobacterium woodii directly catalyzing hydrogenation CO2. We also demonstrate...
Synthesis of acetate from carbon dioxide and molecular hydrogen is considered to be the first assimilation pathway on earth. It combines fixation into acetyl-CoA with production ATP via an energized cell membrane. How coupled net synthesis has been enigma. The anaerobic, acetogenic bacterium Acetobacterium woodii uses ancient version this without cytochromes quinones. generates a sodium ion potential across membrane by sodium-motive ferredoxin:NAD oxidoreductase (Rnf). genome sequence A....
The anaerobic acetogenic bacterium Acetobacterium woodii carries out a unique type of Na + -motive, respiration with caffeate as electron acceptor, termed “caffeate respiration.” Central, and so far the only identified membrane-bound reaction in this pathway, is ferredoxin:NAD oxidoreductase (Fno) activity. Here we show that inverted membrane vesicles A. couple transfer from reduced ferredoxin to NAD transport outside into lumen vesicles. was electrogenic, accumulation inhibited by sodium...
Summary Lactate is a common substrate for major groups of strictly anaerobic bacteria, but the biochemistry and bioenergetics lactate oxidation obscure. The high redox potential pyruvate/lactate pair E 0 ′ = −190 m V excludes direct NAD + reduction ( −320 ). To identify hitherto unknown electron acceptor, we have purified dehydrogenase LDH ) from anaerobic, acetogenic bacterium A cetobacterium woodii . forms stable complex with an electron‐transferring flavoprotein tf) that exhibited only...
Hepatoblastoma is the most common pediatric cancer of liver and majority cases display activating mutations in Wnt/β-catenin pathway. Understanding complex milieu tumor microenvironment has resulted promising new therapies for adult cancers, but similar approaches cancers are still lacking. We aimed to provide a comprehensive analysis hepatoblastoma unveiling its spatial architecture key signaling mechanisms. Single-cell/-nucleus RNA-seq (n=15), transcriptomics (n=22), multiplex...
Aberrant DNA methylation of CpG sites is among the earliest and most frequent alterations in cancer. Several studies suggest that aberrant occurs a tumour type-specific manner. However, large-scale analysis candidate genes has so far been hampered by lack high throughput assays for detection. We have developed first microarray-based technique which allows genome-wide assessment selected dinucleotides as well quantification at each site. hundred were screened 76 samples from four different...
ATP synthases are the primary source of in all living cells. To catalyze synthesis, these membrane-associated complexes use a rotary mechanism powered by transmembrane diffusion ions down concentration gradient. assumed to be driven either H + or Na , reflecting distinct structural motifs their membrane domains, and metabolisms host organisms. Here, we study methanogenic archaeon Methanosarcina acetivorans using assays hydrolysis ion transport inverted vesicles, experimentally demonstrate...
The Wood-Ljungdahl pathway allows acetogenic bacteria to grow on a number of one-carbon substrates, such as carbon dioxide, formate, methyl groups, or even monoxide. Since monoxide alone in combination with hydrogen and dioxide (synthesis gas) is an increasingly important feedstock for third-generation biotechnology, we studied CO metabolism the model acetogen Acetobacterium woodii. When cells grew H2-CO2, addition 5 15% led higher final optical densities, indicating utilization cosubstrate....
The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to reduction NAD+, and negative free energy change this reaction used generate transmembrane ion gradient. In one class anaerobic acetogenic bacteria, believed be essential for conservation autotrophic growth. We describe here methodology markerless mutagenesis in model bacterium class, Acetobacterium woodii, which enabled us delete rnf genes test their vivo role. mutant did not grow on H2 plus CO2,...
The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a and energy source, was adapted grow on CO. This achieved by using preculture grown H2 plus CO2 increasing the CO concentration in small, 10% increments.T. kivui finally able within 100% atmosphere. Growth found complex mineral media, vitamins were required. Carbon consumption accompanied acetate hydrogen production. Cells also grew synthesis gas (syngas) with simultaneous of...