Francesco Chiani
A5016468953- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Connexins and lens biology
- Hearing, Cochlea, Tinnitus, Genetics
- Mitochondrial Function and Pathology
- Genetic and Kidney Cyst Diseases
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Fetal and Pediatric Neurological Disorders
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Insect and Pesticide Research
- Urban Planning and Valuation
- Metabolomics and Mass Spectrometry Studies
- Nicotinic Acetylcholine Receptors Study
- RNA regulation and disease
- Cystic Fibrosis Research Advances
- Bat Biology and Ecology Studies
- Congenital heart defects research
- Congenital Anomalies and Fetal Surgery
- Health, Environment, Cognitive Aging
- Neuroscience and Neuropharmacology Research
- Photodynamic Therapy Research Studies
Institute of Cell Biology and Neurobiology
2015-2024
National Research Council
2015-2024
European Molecular Biology Laboratory
2017-2024
Helmholtz Zentrum München
2017
German Center for Neurodegenerative Diseases
2017
Technical University of Munich
2017
Munich Cluster for Systems Neurology
2017
Università degli Studi della Tuscia
2011
Sapienza University of Rome
2005-2011
Istituto Pasteur
2005
In a search for potent inhibitors of class III histone/protein deacetylases (sirtuins), series sirtinol analogues have been synthesized and the degree inhibition was assessed in vitro using recombinant yeast Sir2, human SIRT1, SIRT2 vivo with phenotypic assay. Two analogues, namely, 3- 4-[(2-hydroxy-1-naphthalenylmethylene)amino]-N-(1-phenylethyl)benzamide (i.e., m- p-sirtinol), were 2- to 10-fold more than against SIRT1 enzymes. assay, these two small molecules as sirtinol. Compounds...
Abstract Despite advances in next generation sequencing technologies, determining the genetic basis of ocular disease remains a major challenge due to limited access and prohibitive cost human forward genetics. Thus, less than 4,000 genes currently have available phenotype information for any organ system. Here we report ophthalmic findings from International Mouse Phenotyping Consortium, large-scale functional screen with goal generating phenotyping null mutant every mouse gene. Of 4364...
Numerous currently incurable human diseases have been causally linked to mutations in connexin (Cx) genes. In several instances, pathological generate abnormally active Cx hemichannels, referred also as "leaky" hemichannels. The goal of this study was assay the vivo efficacy a potent antagonist antibody targeting
ABSTRACT Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder affecting normal structure and function of motile cilia, phenotypically manifested as chronic respiratory infections, laterality defects infertility. Autosomal recessive mutations in genes encoding for different components the axoneme have been associated with PCD humans model organisms. The CCDC151 gene encodes coiled-coil axonemal protein that ensures correct attachment outer dynein arm (ODA) complexes to...
Using microfluidics, ATP biosensors, multiphoton microscopy and genetically targeted mice, we show that release through connexin hemichannels, not pannexin 1 channels, underlies spontaneous Ca<sup>2+</sup> wave propagation in the greater epithelial ridge of developing cochlea.
MicroRNAs (miRNAs) represent a class of small non-coding RNAs that control gene expression by targeting mRNAs and triggering either repression translation or RNA degradation. They have been shown to be involved in variety biological processes such as development, differentiation cell cycle control, but little is known about their involvement the response irradiation. We showed here human umbilical vein endothelial cells (HUVEC) some miRNAs previously crucial role vascular biology are...
Odad3 gene loss-of-function mutation leads to Primary Ciliary Dyskinesia (PCD), a disease caused by motile cilia dysfunction. Previously, we demonstrated that knockout of the in mice replicates several features PCD, such as hydrocephalus, defects left–right body symmetry, and male infertility, with complete absence sperm reproductive tract. The majority animals die before sexual maturation due severe hydrocephalus failure thrive, which precludes fertility studies. Here, performed expression...
In this work, we applied three-dimensional microCT imaging to study murine embryogenesis in the range from immediate post-implantation period (embryonic day 5.5) mid-gestation 12.5) with resolution up 1.4 µm/voxel. Also, introduce an procedure for non-invasive volumetric estimation of entire litter embryos within maternal uterine structures. This method allows accurate, detailed and systematic morphometric analysis both embryonic extra-embryonic components during embryogenesis....
ORIGINAL RESEARCH article Front. Physiol., 11 June 2019Sec. Membrane Physiology and Biophysics Volume 10 - 2019 | https://doi.org/10.3389/fphys.2019.00392
Panx1 forms plasma membrane channels in brain and several other organs, including the inner ear. Biophysical properties, activation mechanisms modulators of have been characterized detail, however impact on auditory function is unclear due to conflicts published results. To address this issue, hearing performance cochlear Panx1−/− mouse strain, first with a reported global ablation Panx1, were scrutinized. Male female homozygous (Panx1−/−), hemizygous (Panx1+/−) their wild type (WT) siblings...
The analysis of the great extent data generated by using DNA microarrays technologies has shown that transcriptional response to radiation can be considerably different depending on quality, dose range and rate radiation, as well timing selected for analysis. At present, it is very difficult integrate obtained under several experimental conditions in biological systems reach overall conclusions or build regulatory models which may tested validated. In fact, most available buried websites,...
The null mutation of the SIR2 gene in Saccharomyces cerevisiae has been associated with a series different phenotypes including loss transcriptional silencing, genome instability and replicative aging. Thus, product is an important constituent yeast cell. orthologues paralogues have discovered organisms ranging from bacteria to man, underscoring pivotal role this protein. Here we report that plasmid introduced into sir2Δ cells accumulates more negative supercoils compared same wild-type (WT)...
Abstract The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for over 5,000 genes from targeted embryonic stem cells on C57BL/6N genetic background. This includes 2,850 null alleles which no equivalent line exists, 2,987 novel conditional-ready alleles, and 4,433 reporter alleles. nearly triples number with almost doubles conditional available to scientific community. When combined more than 30 years community effort, total allele resource covers...
In this study, we used B16-F10 cells grown in the dorsal skinfold chamber (DSC) preparation that allowed us to gain optical access processes triggered by photodynamic therapy (PDT). Partial irradiation of a photosensitized melanoma cell death non-irradiated tumor cells. Multiphoton intravital microscopy with genetically encoded fluorescence indicators revealed bystander was mediated paracrine signaling due adenosine triphosphate (ATP) release from connexin (Cx) hemichannels (HCs)....
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel system’s functions. Three-dimensional micro-CT X-ray with high spatial resolution in minimally perturbed naive non-transparent has recently gained increased popularity broad application biomedical research. Here, we describe novel X-ray-based methodology analysis β-galactosidase...
DNA topoisomerase I together with the other cellular topoisomerases releases torsional stress from caused by processes such as replication, transcription and recombination. Despite well-defined knowledge of its mechanism action, in vivo activity has been only partially characterized. In fact basic question concerning capability enzyme to cleave rejoin wrapped around a histone octamer remains still unanswered. By studying both vitro cleavage presence camptothecin on repeated trinucleotide...
Abstract Acquisition of detailed structural and molecular information from intact biological samples, while preserving cellular three-dimensional structures, still represents a challenge for studies aiming to unravel system functions. Here we describe novel X-ray-based methodology analysis gene expression pattern in murine brain ex vivo by microCT. The method relays on detection bromine molecules the products enzymatic reaction generated β -galactosidase (lacZ) reporter. To demonstrate...