A5000610455- Thyroid Disorders and Treatments
- Thyroid Cancer Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Bone health and treatments
- Plant Water Relations and Carbon Dynamics
- Analytical Methods in Pharmaceuticals
- Growth Hormone and Insulin-like Growth Factors
- Advanced Drug Delivery Systems
- Antibiotics Pharmacokinetics and Efficacy
- Hormonal and reproductive studies
- Plant responses to elevated CO2
- Pharmacological Effects and Toxicity Studies
- Radiation Dose and Imaging
- Neuroendocrine Tumor Research Advances
- Thyroid and Parathyroid Surgery
- Advancements in Transdermal Drug Delivery
- Drug Transport and Resistance Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Metabolism and Genetic Disorders
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Atrial Fibrillation Management and Outcomes
- Pharmaceutical studies and practices
Helmholtz Zentrum München
2005-2021
Diakonhjemmet Hospital
2019
University of Sheffield
2017
Jackson Laboratory
2016
Intermountain Healthcare
2012-2013
Leo Pharma (Denmark)
2004-2012
Technical University of Munich
2012
Leo Pharma (Australia)
2010-2012
Max Planck Innovation
2010
Max Planck Society
2010
In 2007, the International Knockout Mouse Consortium (IKMC) made ambitious promise to generate mutations in virtually every protein-coding gene of mouse genome a concerted worldwide action. Now, 5 years later, IKMC members have developed high-throughput trapping and, particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones 1,700 strains, most them conditional. A common web portal (www.knockoutmouse.org) has been established, allowing...
We assessed extravascular accumulation of albumin and fluid in primary myxedema by measuring metabolic turnover transcapillary escape 131I-labeled human seven patients. In the hypothyroid state, we found a low plasma volume (P less than 0.05), reduced rate synthesis catabolism 0.01), an increased remarkable increase mass (1500 micronmol; P 0.01) longer mean transit time through spaces other states generalized edema 0.05). All variables returned to normal during l-thyroxine treatment. The...
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in most genes expressed mouse embryonic stem (ES) cells. The rely on directional site-specific recombination systems repair and reinduce when activated succession. After the traps are inserted into genome, genetic be produced at a particular time place somatic In addition to their features, create multipurpose alleles amenable wide range postinsertional modifications. Here we have used these...
HLA typing of 86 patients with Graves' disease was performed for the A, B, C and D series antigens. An increased frequency HLA-B8 (47 per cent) Dw3 (51 compared controls (23.7 21 cent, respectively) observed. The increase B8 almost exclusively found in a group 48 relapse disease, whereas without did not differ significantly from that control group. No association presence exophthalmos, thyroid antibodies, or antibodies to Yersinia enterocolitica serotype 3 could be found.
The study of genetic disease mechanisms relies mostly on targeted mouse mutants that are derived from engineered embryonic stem (ES) cells. Nevertheless, the establishment mutant ES cells is laborious and time-consuming, restricting increasing number human mutations discovered by high-throughput genomic analysis. Here, we present an advanced approach for production models microinjection transcription activator-like effector nucleases (TALENs) synthetic oligodeoxynucleotides into one-cell...
SUMMARY The administration of sulthiame in doses 200–800 mg daily to patients receiving diphenylhydantoin caused a rise serum after 1 week combined treatment. In the mentioned above increases half‐life blood injected radioactive diphenylhydantoin. Sulthiame apparently inhibits metabolism liver, but it has not yet been possible elucidate mode this inhibition. RÉSUMÉ L'administration de à des quotidiennes 200 800 chez recevant d'autre part la diphénylhydantoine provoquait au bout d'une semaine...
Summary OBJECTIVE The persistent controversy as to the best approach radioiodine dose selection in treatment of hyperthyroldism led us perform a study order compare fixed regime comprising doses 185, 370 or 555 MBq based on gland size assessment by palpation only, with calculated 131 I type thyroid (diffuse, multinodular, solitary adenoma), an accurate volume measurement, and 24‐hour uptake determination. DESIGN Prospective randomized study. PATIENTS Two hundred twenty‐one consecutive...
OBJECTIVE To investigate the long‐term effects of radioiodine ( 131 I) on thyroid function and size in patients with a solitary toxic nodule. DESIGN Prospective study treated for autonomous nodule, followed by evaluation volume function. PATIENTS Sixty‐two consecutive minimum 12 months (range 12–168, median 60). Seventeen received antithyroid drug treatment before I. MEASUREMENTS Standard variables ultrasonically determined as well 0.75, 1.5, 3, 6 after treatment, then once year were...
A major challenge of the postgenomic era is functional characterization every single gene within mammalian genome. In an effort to address this challenge, we assembled a collection mutations in mouse embryonic stem (ES) cells, which largest publicly accessible such date. Using four different gene-trap vectors, generated 5,142 sequences adjacent integration sites (gene-trap sequence tags; http://genetrap.de) from >11,000 ES cell clones. Although most vector insertions occurred randomly...
Abstract Histone deacetylases (HDAC) reverse the acetylation of histone and nonhistone proteins thereby modulate chromatin structure function proteins. Many tumor cell lines experimental tumors respond to HDAC inhibition. To assess role an individual isoenzyme in physiology development, HDAC2-mutant mice were generated from a gene trap embryonic stem clone. These express catalytically inactive fusion protein NH2-terminal part HDAC2 β-galactosidase, which fails integrate into corepressor...
The International Knockout Mouse Consortium (IKMC) aims to mutate all protein-coding genes in the mouse using a combination of gene targeting and trapping embryonic stem (ES) cells make generated resources readily available research community. IKMC database web portal ( www.knockoutmouse.org ) serves as central public site for data facilitates coordination prioritization work within consortium. Researchers can access up-to-date information on knockout vectors, ES mice specific genes, follow...
Abstract Background MicroRNAs (miRNAs) are post-transcriptional regulators involved in numerous biological processes including the pathogenesis of Alzheimer’s disease (AD). A key gene AD, ADAM10 , controls proteolytic processing APP and formation amyloid plaques is known to be regulated by miRNA hepatic cancer cell lines. To predict miRNAs regulating expression concerning we developed a computational approach. Methods MiRNA binding sites human 3' untranslated region were predicted using...
The effect of diphenylhydantoin (DPH) on serum T4, a free T4 index, T3, TSH, TSH response to iv TRH and absolute iodine uptake (AIU) the thyroid gland was studied in 26 patients receiving long-term DPH treatment compared controls. In DPH-treated group, index T3 were decreased 75% control values. Serum AIU significantly increased. Twelve before after 1–2 weeks with significant decrease noted. but not significantly. increased, however, increase statistically significant. 7 hyperthyroidism 2–4...
The use of 131I treatment in nodular toxic goiter is widely accepted. In this article, we describe transition into an autoimmune with development thyrotropin receptor antibodies (TRAb) as a side effect treatment. retrospective study, 149 patients (100 multinodular goiter, 49 solitary autonomously functioning nodule) were studied. Of these 100 became permanently euthryoid after 1 dose 131I, and due to persistent hyperthyroidism, 32 needed 2-5 doses euthyroid. After becoming euthyroid, none...