A5052604833- Ion Transport and Channel Regulation
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Nitric Oxide and Endothelin Effects
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Neuroscience and Neuropharmacology Research
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Cardiomyopathy and Myosin Studies
- Ion channel regulation and function
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Pancreatic function and diabetes
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Phosphodiesterase function and regulation
- Receptor Mechanisms and Signaling
- Nerve injury and regeneration
- Cellular Mechanics and Interactions
Aarhus University
2015-2024
Institut thématique Génétique, génomique et bioinformatique
2016
Lithuanian University of Health Sciences
2011
Aarhus University Hospital
2002-2009
Institute of Molecular Biology of the Slovak Academy of Sciences
2007
Copenhagen University Hospital
2006
Max Planck Institute for Developmental Biology
2003
Goethe University Frankfurt
2003
Technische Universität Braunschweig
2003
Johns Hopkins University
2002
Enteroendocrine cells such as duodenal cholecystokinin (CCK cells) are generally thought to be confined certain segments of the gastrointestinal (GI) tract and store release peptides derived from only a single peptide precursor. In current study, however, transgenic mice expressing enhanced green fluorescent protein (eGFP) under control CCK promoter demonstrated distribution pattern CCK-eGFP positive that extended throughout intestine. Quantitative PCR liquid chromatography-mass spectrometry...
Pregnancy-associated plasma protein A (PAPPA) is a metzincin superfamily metalloproteinase in the insulin-like growth factor (IGF) system. PAPPA increases IGF bioavailability and mitogenic effectiveness vitro through regulated cleavage of IGF-binding 4 (IGFBP4). To determine its function vivo, we generated PAPPA-null mice by gene targeting. Mice homozygous for targeted disruption were viable but 60% size wild-type littermates at birth. The impact mutation was exerted during early embryonic...
Aquaporin-9 (AQP9) is an aquaglyceroporin membrane channel shown biophysically to conduct water, glycerol, and other small solutes. Because the physiological role/s of AQP9 remain undefined expression sites incomplete conflicting, we generated knockout mice. In absence stress, mice did not display any visible behavioral or severe physical abnormalities. Immunohistochemical analyses using multiple antibodies revealed specific labeling in hepatocytes, epididymis, vas deferens, epidermis wild...
Cerebral water accumulation was studied during induction of brain edema in dystrophin-null transgenic mice ( mdx- β geo ) and control mice. Immunofluorescence immunoelectron microscopic analyses brains revealed a dramatic reduction AQP4 (aquaporin-4) astroglial end-feet surrounding capillaries (blood–brain barrier) at the glia limitans (cerebrospinal fluid–brain interface). The protein is mislocalized, because immunoblotting showed that total abundance unaltered. Brain induced by i.p....
Background —We studied the effects of angiotensin II (Ang II) and diastolic overstretch on induction cardiac growth in isometrically contracting muscle preparations from human right atria left ventricles. We used gene expression brain natriuretic peptide (BNP) as a molecular marker hypertrophy. Methods Results —Northern blot analysis was performed atrial preparations, which were either incubated 10 −6 mol/L Ang for 45 minutes or diastolically stretched to 120% optimum length. Similar...
Abstract The differential expression of genes triggering myogenesis might cause or reflect differences among myoblasts. Little is known about the presence MyoD1 and myogenin during process regeneration. We therefore examined in muscle regeneration after grafting. Immunostaining regenerating skeletal mouse revealed both myogenin. In mononucleated cells proteins were not detected until shortly before fusion into myotubes. They persisted nuclei regenerated fibers for at least 2 weeks....
Retroviruses have been invading mammalian germlines for millions of years, accumulating in the form endogenous retroviruses (ERVs) that account nearly one-tenth mouse and human genomes. ERVs are epigenetically silenced during development, yet cellular factors recognizing a sequence-specific manner remain elusive. Here we demonstrate ZFP809, member Krüppel-associated box zinc finger protein (KRAB-ZFP) family, initiates silencing via recruitment heterochromatin-inducing complexes. ZFP809...
Aquaporin-2 (AQP2) is the predominant vasopressin-regulated water channel in kidney connecting tubule (CNT) and collecting duct (CD) essential for renal regulation of body balance. However, relative role AQP2 to urinary concentration CNT CD segments unknown. To examine this directly, transgenic mice expressing selectively but lacking expression (AQP2-CD-KO) globally (AQP2-total-KO) were generated by exploiting Cre/loxP technology. LoxP sites inserted into introns 2 3, bred with strains Cre...
A familial AD mutation in SORL1 disrupts the ability of sorting receptor SORLA to mediate intracellular degradation Aβ peptides.
Fibrous cap smooth muscle cells (SMCs) protect atherosclerotic lesions from rupturing and causing thrombosis, while other plaque SMCs may have detrimental roles in development. To gain insight into recruitment of different SMCs, we mapped their clonal architecture aggregation chimeras eGFP+Apoe–/– Apoe–/– mouse embryos mice with a mosaic expression fluorescent proteins medial that were rendered by PCSK9-induced hypercholesterolemia. caps found constructed large, endothelial-aligned layers...
Mammalian stanniocalcin-2 (STC2) is a secreted polypeptide widely expressed in developing and adult tissues. However, although transgenic expression mice known to cause severe dwarfism, targeted deletion of STC2 causes increased postnatal growth, its precise biological role still unknown. We found that potently inhibits the proteolytic activity growth-promoting metalloproteinase, pregnancy-associated plasma protein-A (PAPP-A). Proteolytic inhibition requires covalent binding PAPP-A mediated...
Background— Disturbances in pH affect artery function, but the mechanistic background remains controversial. We investigated whether Na + ,HCO 3 − cotransporter NBCn1, by regulating intracellular (pH i ), influences function and blood pressure regulation. Methods Results— Knockout of NBCn1 mice eliminated cotransport caused a lower steady-state mesenteric smooth muscle endothelial cells situ evaluated fluorescence microscopy. Using myography, arteries from knockout showed reduced...
The murine homologue of the Drosophila twist gene has been shown to be essential for head mesenchyme formation and act as an inhibitor muscle differentiation. This paper presents a detailed analysis M-twist expression patterns from day 7 post coitum (p.c.) 18 p.c., indicating more general function gene. At is expressed in mesoderm outside primitive streak. Later message predominantly found somites, mesenchyme, branchial arches, limbs, underneath epidermis. Beginning at 8 mainly...
A major challenge of the postgenomic era is functional characterization every single gene within mammalian genome. In an effort to address this challenge, we assembled a collection mutations in mouse embryonic stem (ES) cells, which largest publicly accessible such date. Using four different gene-trap vectors, generated 5,142 sequences adjacent integration sites (gene-trap sequence tags; http://genetrap.de) from >11,000 ES cell clones. Although most vector insertions occurred randomly...
Aquaporin (AQP7) is expressed in proximal tubules and involved glycerol uptake. The cellular expression physiological function other organs remain largely undefined. AQP7 knockout (KO) mice were generated used for immunohistochemical analyses to define the organ of AQP7. labeling was found kidney tubule, heart, skeletal muscle, testis, epididymis, as well white brown adipose tissue (WAT BAT) wild-type mice. Importantly, immunoreactivity completely absent from these tissues KO At level,...
The mitochondrial Hsp60 chaperonin plays an important role in sustaining cellular viability. Its dysfunction is related to inherited forms of the human diseases spastic paraplegia and hypomyelinating leukodystrophy. However, it unknown whether requirement for neuron specific or a complete loss protein will impair mammalian development postnatal survival. In this study, we describe generation characterization mutant mouse line bearing inactivating gene-trap insertion Hspd1 gene encoding...
Familial idiopathic basal ganglia calcification (FIBGC) is a neurodegenerative disorder with neuropsychiatric and motor symptoms. Deleterious mutations in SLC20A2, encoding the type III sodium-dependent phosphate transporter 2 (PiT2), were recently linked to FIBGC almost 50% of families reported worldwide. Here, we show that knockout Slc20a2 mice causes calcifications thalamus, ganglia, cortex, demonstrating reduced PiT2 expression alone can cause brain calcifications.
Objective— Arterial injury stimulates remodeling responses that, when excessive, lead to stenosis. These are influenced by integrin signaling in vascular smooth muscle cells (VSMCs). Microfibrillar-associated protein 4 (MFAP4) is an ligand localized extracellular matrix fibers the wall. The role of MFAP4 biology unknown. We aimed test hypothesis that would enhance integrin-dependent VSMC activation. Approach and Results— produced Mfap4 -deficient ( −/− ) mice performed carotid artery...
Abstract Migraine is a complex brain disorder, and understanding the complexity of this prevalent disease could improve quality life for millions people. Familial Hemiplegic type 2 (FHM2) subtype migraine with aura co-morbidities like epilepsy/seizures, cognitive impairments psychiatric manifestations, such as obsessive-compulsive disorder (OCD). FHM2 disease-mutations locate to ATP1A2 gene encoding astrocyte-located α -isoform sodium-potassium pump (α Na + /K -ATPase). We show that knock-in...
Autophagy-mediated degradation of synaptic components maintains homeostasis but also constitutes a mechanism neurodegeneration. It is unclear how autophagy vesicles and presynaptic active zones regulated. Here, we show that Pleckstrin homology containing family member 5 (Plekhg5) modulates in axon terminals motoneurons via its function as guanine exchange factor for Rab26, small GTPase specifically directs to preautophagosomal structures. Plekhg5 gene inactivation mice results late-onset...