Aquaporin (AQP7) is expressed in proximal tubules and involved glycerol uptake. The cellular expression physiological function other organs remain largely undefined. AQP7 knockout (KO) mice were generated used for immunohistochemical analyses to define the organ of AQP7. labeling was found kidney tubule, heart, skeletal muscle, testis, epididymis, as well white brown adipose tissue (WAT BAT) wild-type mice. Importantly, immunoreactivity completely absent from these tissues KO At level,...

In mammals, the majority of nitrogen from protein degradation is disposed as urea. Several studies have partly characterized expression urea transporters (UTs) in hepatocytes, where produced. Nevertheless, contribution these proteins to hepatocyte permeability (P(urea)) and their role liver physiology remains unknown. The purpose this study was biophysically examine transport. We hypothesized that water, glycerol, channel aquaporin-9 (AQP9) involved release. Stopped-flow light-scattering...

Insulin resistance and metabolic inflexibility are features of obesity amplified by fasting. Growth hormone (GH) secretion increases during fasting GH causes insulin resistance. To study the effects blockade in obese subjects. Nine males were studied thrice a randomized design: (1) after an overnight fast (control), (2) 72 hour (fasting), (3) with (pegvisomant) [fasting plus antagonist (GHA)]. Each day consisted 4-hour basal period followed 2-hour hyperinsulinemic, euglycemic clamp combined...

Obesity is associated with increased plasma glycerol levels. The coordinated regulation of channels in adipose tissue (AQP7) and the liver (AQP9) has been suggested as an important contributor to pathophysiology type-2-diabetes mellitus, it would provide for hepatic synthesis glucose triglycerides. AQP7 AQP9 influenced by sex. This study investigates effect a high-fat diet (HFD) on metabolism mice influence sex or GLP-1-agonist treatment. Female male C57BL/6JRj were fed either control HFD 12...

In starvation, glycerol is released from adipose tissue and serves as an important precursor for hepatic gluconeogenesis. By unknown sex-specific mechanisms, women suppress the endogenous glucose production better than men respond to metabolic stress with higher plasma levels. Hepatic uptake facilitated by aquaporin-9 (AQP9), a broad-selectivity neutral solute channel, represents insulin-regulated step in supplying gluconeogenesis glycerol. present study, AQP9 abundance was increased...

The peroxisome proliferator receptor α (PPARα) is a key regulator of the hepatic response to fasting with effects on both lipid and carbohydrate metabolism. A role in glycerol metabolism has also been found; however, results are somewhat contradictive. Aquaporin 9 (AQP9) pore-forming transmembrane protein that facilitates uptake glycerol. Its expression inversely regulated by insulin male rodents, increased during fasting. Previous indicate PPARα plays crucial induction AQP9 mRNA In present...

Aquaglyceroporin 7 (AQP7) facilitates the transport of glycerol across cell membranes. In mice, fasting and refeeding regulate adipose tissue AQP7 abundance, a role in controlling triglyceride accumulation has been proposed. is also expressed skeletal muscle, where its function remains to be determined. Here, abundance abdominal subcutaneous (SAT) muscle was evaluated overnight fasted postprandial state eight lean obese men with type 2 diabetes (T2D). A biopsy from SAT collected after an...

Abstract Studies in obese rats and human cell models of non‐alcoholic fatty liver disease have indicated that knockdown the hepatic glycerol channel aquaporin 9 (AQP9) leads to decreased steatosis. However, a study leptin receptor‐deficient mice did not find knockout (KO) AQP9 alleviated The aim this was investigate effect high‐fat diet (HFD) on triglyceride metabolism male female KO mice. Male wild‐type (WT) littermates were fed HFD for 12 weeks. Weight, food intake blood glucose monitored...

Diabetes mellitus (DM) is associated with a significant polyuria and natriuesis as well increased plasma aldosterone anti-diuretic hormone arginine vasopressin (AVP). This study aimed to determine whether diabetic kidneys compensate for the urinary sodium water losses by increasing apical targeting of epithelial channel (ENaC) subunits aquaporin-2 (AQP2) in collecting duct, addition previously observed changes ENaC subunit protein expression different kidney zones.Female rats were...

When plasma triglyceride is assessed in standard laboratories, it a measurement of glycerol after hydrolysis triglycerides into fatty acids and glycerol. In most patients, the level free will only marginally influence triglyceride. However, rare cases elevated concentrations causes pseudohypertriglyceridemia blanking for becomes important. this study, we investigated 100 adult men with mild to moderate hypertriglyceridemia assess need providing our clinical biochemistry department. The...

Aim: To test the effects of dietary NaCl and carbohydrate content on urine volume in diabetic rats. Methods: Streptozotocin-induced rats were subjected to restriction using either a NaCl-deficient carbohydrate-rich synthetic diet (Altromin C1036) supplemented contain 0.16% (C1036 + lowNaCl) or modified normal cereal-based 1320) containing 0.086% (lowNaCl-1320). Normal contained 0.2683% NaCl. Results: Using C1036 lowNaCl diet, earlier reported paradoxical increases water intake reproduced....

In adipose tissue, reduced expression of the glycerol channel aquaporin 7 (AQP7) has been associated with increased accumulation triglyceride. The present study determines relative protein abundances lipolytic enzymes, AQP7, and cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) in paired mesenteric omental visceral tissue (VAT) abdominal femoral subcutaneous (SAT) women either normal weight or upper-body obesity. No differences hormone-sensitive lipase (HSL) AQP7 were found between two...

Glycerol is known as a product of lipolysis and an important precursor for hepatic gluconeogenesis. Nevertheless, the regulation glycerol metabolism far from fully elucidated. Aquaporin-9 (AQP9) has been linked to uptake glycerol, inside cell glyceriol converted glycerol-3-phosphate by kinase (GK). We investigated effect starvation on initial found that AQP9 GK are regulated in gender specific manner. Starved male rats increased expression both ~ 2-fold, whereas no changes were observed...

One week after induction of uncontrolled diabetes mellitus (DM) in female rats by streptozotocin injection, the effect dietary NaCl restriction on urinary volume (U V ) was investigated. In first experiment (n=6 each group), were offered a synthetic NaCl‐deficient diet (C1036) for 2 days. DM rats, U increased dramatically (111±2 vs 211±7 ml/day; p<0.001), whereas non‐diabetic controls showed no change (13.1±1.9 11.7±1.5 p=0.6). second (n=5 receiving C1036 days again dramatic increase ,...

Increased hepatic glucose production plays a pivotal role in the pathophysiology of diabetes. AQP9 has been suggested to facilitate transport glycerol into hepatocytes where kinase (GK) catalyses initial step for its conversion either or glycerolipids. Hence, regulation and thereby uptake could be potential modulator production. This study aims investigate gender GK protein. Immunoblotting showed that starvation male rats resulted increased abundance, whereas, starved female displayed no...

In mammals, nitrogen from protein degradation is disposed as urea. Several studies have investigated expression of urea transporters in hepatocytes, where produced. Nevertheless, the role facilitated transport liver remains unknown. presented study we determine permeability isolated basolateral hepatocyte membranes by stopped flow light scattering measurements. The channel inhibitors phloretin and dimethylurea reduced 70% 40%, respectively. AQP9 (AQP9−/−) UT‐A (UT‐A−/−) single knockout,...

It has previously been shown that male rats have higher hepatic expression of AQP9 than female (Nicchia et al, J Histochem Cytochem, 2001). The regulation also related to PPARα (Patsouris Clin Invest, 2004). This study aims investigate the role castration and PPARα–agonist (Wy 14643) administration in protein rats. In castrated rats, immunoblotting showed a 1.7 fold increase (p < 0.01). finding was confirmed by immunohistochemical analysis. Serum testosterone decreased group 0.01), while...

During fasting triglyceride (TG) in adipose tissue (AT) is hydrolyzed into fatty acids and glycerol. Glycerol exits AT through the glycerol channel aquaporin 7 (AQP7). AQP7 KO mice have increased activity of kinase (GlyK), storage TG, become obese indicating that changes metabolism influences TG storage. Moreover, humans an association between low expression obesity has been found women only. This study aims to investigate whether high fat diet (HFD) a sex-specific manner influenced by GLP-1...