A5017575108- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Geological and Geophysical Studies
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Geological Studies and Exploration
- Hydrocarbon exploration and reservoir analysis
- Chromosomal and Genetic Variations
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Global Financial Crisis and Policies
- RNA and protein synthesis mechanisms
- Banking stability, regulation, efficiency
- Natural Compounds in Disease Treatment
- Genetic Mapping and Diversity in Plants and Animals
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Neuroscience and Music Perception
- Genetic and phenotypic traits in livestock
- Growth Hormone and Insulin-like Growth Factors
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Systemic Lupus Erythematosus Research
Rice University
2024-2025
Heilongjiang Bayi Agricultural University
2025
Shanghai Tenth People's Hospital
2016-2024
Tongji University
2016-2024
Affiliated Hospital of Guizhou Medical University
2024
Guizhou University
2024
Guangzhou University of Chinese Medicine
2023
Chinese Center For Disease Control and Prevention
2023
Shantou University
2023
Nanjing Medical University
2023
Retroviruses have been invading mammalian germlines for millions of years, accumulating in the form endogenous retroviruses (ERVs) that account nearly one-tenth mouse and human genomes. ERVs are epigenetically silenced during development, yet cellular factors recognizing a sequence-specific manner remain elusive. Here we demonstrate ZFP809, member Krüppel-associated box zinc finger protein (KRAB-ZFP) family, initiates silencing via recruitment heterochromatin-inducing complexes. ZFP809...
Myocyte nuclear factor (MNF) is a winged helix transcription that expressed selectively in myogenic stem cells (satellite cells) of adult animals. Using gene knockout strategy to generate functional null allele at the Mnf locus, we observed mice lacking MNF are viable, but severely runted. Skeletal muscles −/− animals atrophic, and satellite cell function impaired. Muscle regeneration after injury delayed incomplete, normal timing expression cycle regulators determination genes dysregulated....
Histone demethylase LSD1 can form complex with different Rcor family corepressors in cell types. It remains unknown if cell-specific proteins function specifically distinct Here, we report that Rcor2 is predominantly expressed ESCs and forms a facilitates its nucleosomal demethylation activity. Knockdown of inhibited ESC proliferation severely impaired the pluripotency. Moreover, knockdown greatly formation induced pluripotent stem (iPS) cells. In contrast, ectopic expression somatic cells...
Proteins dynamically interact with each other to perform their biological functions. The dynamic operations of protein interaction networks (PPI) are also reflected in the formations complexes. Existing complex detection algorithms usually overlook inherent temporal nature interactions within PPI networks. Systematically analyzing complexes can not only improve accuracy detection, but strengthen our knowledge on assembly processes for cellular organization.In this study, we propose a novel...
Abstract Epigenetic regulatory complexes play key roles in the modulation of transcriptional regulation underlying neural stem cell (NSC) proliferation and progeny specification. How specific cofactors guide histone demethylase LSD1/KDM1A complex to regulate distinct NSC-related gene activation repression cortical neurogenesis remains unclear. Here we demonstrate that Rcor2, a co-repressor LSD1, is mainly expressed central nervous system (CNS) plays role epigenetic development. Depletion...
Embryo viability relies on placental repression The insulin-like growth factor (IGF) signaling pathway controls maternal supply of and fetal demands for nutrients. Yang et al. report that the essential KRAB–zinc finger protein ZFP568 specifically directly represses a placental-specific IGF2 transcript during early mouse development. Elimination in vivo leads to inappropriate activation transcription, which results embryonic death owing overexpression peptide. Thus, specific, targeted...
Abstract Objectives In the current study, for first time, we reported a novel HCV molecular diagnostic approach termed reverse transcription loop-mediated isothermal amplification integrated with gold nanoparticles-based lateral flow biosensor (RT-LAMP-AuNPs-LFB), which developed rapid, sensitive, specific, simple, and visual identification of HCV. Methods A set LAMP primer was designed according to 5’untranslated region (5’UTR) gene from major genotypes 1b, 2a, 3b, 6a, 3a, are prevalent in...
Meiotic recombination hotspots play important roles in various aspects of genomics, but the underlying mechanisms for regulating locations and strengths are not yet fully revealed. Most existing algorithms estimating rates from sequence polymorphism data can only output average a population, although there is evidence heterogeneity among individuals. For genome-wide association studies (GWAS) hotspots, an efficient algorithm that estimates individualized highly desirable. In this work, we...
Skeletal muscle is an essential tissue for maintaining the body's basic functions. The structural unit of skeletal fiber, and its type main factor that determines athletic ability animals. O-linked N-acetylglucosamine (O-GlcNAc) modification, a reversible protein post-translational involved in many important biological processes such as gene transcription, signal transduction, cell growth, differentiation. Myogenic differentiation (MyoD), first discovered myogenic regulatory factor,...
Celastrol, a triterpenoid isolated from stem (<i>caulis</i>) of <i>Celastrus orbiculatus</i> Thunb. (Celastraceae), has been known to have various pharmacological effects, including anti-inflammatory, anticancer, and antioxidant activities. However, the mechanism intestinal absorption celastrol is unknown. The aim this study was investigate using Caco-2 cell transwell model. First, bidirectional transport in monolayers observed. Then, effects time, concentration, temperature, paracellular...
Human naive pluripotent stem cells established from the epiblasts of preimplantation blastocysts provide a useful model for mechanistic studies pluripotency regulation and lineage differentiation. Important advances have been made to optimize culture conditions define molecular criteria pluripotency. However, identity naive-specific surface markers underlying mechanism remain poorly understood. Here, we identify alkaline phosphatase placental-like 2 (ALPPL2) as prominent marker by systematic...
Distal arthrogryposis type 2B (DA2B) is an important genetic disorder in humans. However, the mechanisms governing this disease are not clearly understood. In study, we generated knock-in mice carrying a DA2B mutation (K175del) troponin I 2 (skeletal, fast) (TNNI2), which encodes fast-twitch skeletal muscle protein. Tnni2K175del (referred to as mice) showed typical phenotypes, including limb abnormality and small body size. current knowledge concerning TNNI2 could explain phenotype of mice....
Activation of naïve B lymphocytes by extracellular ligands, e.g. antigen, lipopolysaccharide (LPS) and CD40 ligand, induces a combination common ligand-specific phenotypic changes through complex signal transduction pathways. For example, although all three these ligands induce proliferation, only stimulation the cell antigen receptor (BCR) apoptosis in resting splenic cells. In order to define unique biological responses ligand stimulation, we compared gene expression induced normal primary...
The progression of systemic lupus erythematosus (SLE) leads to anemia in patients, adversely affecting prognosis. diverse causes may include excessive eryptosis or premature suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine (PS) exposure on the surface. present study explored if SLE enhances underlying mechanisms.Eryptosis was assessed using flow cytometry healthy volunteers (n = 20) anemic patients hospitalized for 22), parameters including PS exposure,...
SignificanceHosts often target the relatively conserved regions in rapidly mutating retroviruses to inhibit their replication. One of these is called a primer binding site (PBS), which has be complementary host tRNA initiate reverse transcription. By analyzing endogenous retroviral elements, we found that cells use this sequence as efforts block expression viral elements. A specific type zinc finger protein targets PBS genome, not only inhibits transcription viruses but also replication...
Genetic recombination is an essential event during the process of meiosis resulting in exchange segments between paired chromosomes. Estimating rate crucial for understanding recombination. Experimental methods are normally difficult and limited to small scale estimations. Thus statistical using population genetics data important large-scale analysis. LDhat extensively used method rjMCMC algorithm predict rates. Due complexity scheme, may take a long time large SNP sets. In addition,...
Vitamin A deficiency (VAD) occurs in obesity and may be associated with thyroid dysfunction. We aimed to investigate the association of VA function after laparoscopic sleeve gastrectomy (LSG). Nine hundred seventy-six obese subjects were enrolled for this study divided into VAD, marginal vitamin (MVAD), normal (NVA) groups. VAD was defined as ≤ 200 ng/ml, MVAD > but <300 NVA ≥ 300 ng/ml. Thyroid compared among groups relationship analyzed. Two forty-four 976 underwent LSG, change at 3, 6, 12...