A5038933168- Cancer Cells and Metastasis
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Corneal Surgery and Treatments
- Renal and related cancers
- Electrospun Nanofibers in Biomedical Applications
- Ocular Surface and Contact Lens
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Thyroid Cancer Diagnosis and Treatment
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Drug Transport and Resistance Mechanisms
- Hemoglobin structure and function
- Corneal surgery and disorders
- MicroRNA in disease regulation
- FOXO transcription factor regulation
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Ocular Disorders and Treatments
- Cancer Genomics and Diagnostics
- Cardiac Fibrosis and Remodeling
Newcastle University
2015-2024
Centre for Life
2012-2023
University of Dundee
2021
University of Exeter
2021
University of Newcastle Australia
2021
University of Birmingham
2012
The University of Texas Southwestern Medical Center
2000-2007
New York University
1996-1999
University Medical Center
1996-1999
NYU Langone Health
1996
Myocyte nuclear factor (MNF) is a winged helix transcription that expressed selectively in myogenic stem cells (satellite cells) of adult animals. Using gene knockout strategy to generate functional null allele at the Mnf locus, we observed mice lacking MNF are viable, but severely runted. Skeletal muscles −/− animals atrophic, and satellite cell function impaired. Muscle regeneration after injury delayed incomplete, normal timing expression cycle regulators determination genes dysregulated....
Abstract The pupillary membrane (PM) is a transient ocular capillary network, which can serve as model system in to study the mechanism of regression. Previous work has shown that there tight correlation between cessation blood flow segment and appearance apoptotic cells throughout segment. This pattern cell death referred synchronous apoptosis (Lang, R. A., Lustig, M., Francois, F., Sellinger, M. Plesken, H. (1994) Development 120, 3395-3404; Meeson, Palmer, Calfon, Lang, A. (1996) 122,...
ABSTRACT Previous analyses of developmentally programmed capillary regression suggested two distinct causes vascular endothelial cell (VEC) death. The first appeared to be macrophage-dependent (Lang, R. A. and Bishop, M. J. (1993) Cell 74, 453-462) while the second was proposed result from cessation blood flow A., Lustig, M., Francois, F., Sellinger, Plesken, H. (1994). Development 120, 3395-3403). Combined, these a model in which initial, macrophage-mediated apoptosis blocked within segment...
Myoglobin is a cytoplasmic hemoprotein that restricted to cardiomyocytes and oxidative skeletal myofibers facilitates oxygen delivery during periods of high metabolic demand. content in muscle increases response hypoxic conditions. However, we previously reported myoglobin-null mice are viable fertile. In the present study, define important functional, cellular, molecular compensatory adaptations absence myoglobin. Mice without myoglobin manifest include fiber type transition (type I II...
Muscle progenitor cells (satellite cells) function in the maintenance and repair of adult skeletal muscle. Side population (SP) are enriched repopulating activity also reside In this study, we observed that Abcg2 is a determinant SP cell phenotype. Using reverse transcription polymerase chain reaction immunohistochemical techniques, localized Abcg2-expressing interstitium close approximation to vasculature able differentiate into myotubes increase number after cardiotoxin-induced muscle...
Despite the ability of myogenic progenitor cells (MPCs) to completely regenerate skeletal muscle following injury, little is known regarding molecular program that regulates their proliferation and differentiation. Although mice lacking cyclin-dependent kinase inhibitor p21 (p21-/-), develop normally, we report here p21-/- MPCs display increased cell number enhanced cycle progression compared with wild-type MPCs. Therefore, hypothesized would demonstrate temporally regeneration myotrauma. In...
Abstract —Mice lacking myoglobin survive to adulthood and meet the circulatory demands of exercise pregnancy without cardiac decompensation. In present study, we show that many myoglobin-deficient embryos die in utero at midgestation with signs failure. Fetal mice gestational day 12.5, however, suffer no subsequent excess mortality. Survival absence is associated increased vascularity induction genes encoding hypoxia-inducible transcription factors 1α 2, stress proteins such as heat shock...
In breast cancer it is never the primary tumour that fatal; instead development of metastatic disease which major cause related mortality. There accumulating evidence suggests Cancer Stem Cells (CSC) may play a role in and progression. Breast stem cell populations, including side population cells (SP), have been shown to be primitive cell-like being long-lived, self-renewing highly proliferative. SP are identified using dual wavelength flow cytometry combined with Hoechst 33342 dye efflux,...
The majority of deaths in ovarian cancer are caused by recurrent metastatic disease which is usually multidrug resistant. This progression has been hypothesised to be due part the presence stem cells, a subset cells capable self-renewal and able survive chemotherapy migrate distant sites. Side population (SP) identified efflux DNA-binding dye Hoechst 33342 through ATP-binding cassette (ABC) transporters, known adult cell group have suggested as various cancers. Despite identification SP...
The X-linked transcription factor FOXP3 is expressed by epithelial cells of organs including the breast, where it considered a tumour suppressor. chemokine receptor CXCR4 also regulates development breast cancer stimulating cell migration towards CXCL12-expressing sites metastatic spread. During activation, human T show reciprocal regulation and CXCR4. This study was designed to examine role plays in cancer, with particular focus on its potential regulate Human samples showed significantly...
Previous studies have suggested that the heart may be capable of limited repair and regeneration in response to a focal injury, while other indicate mammalian has no regenerative capacity. To further explore this issue, we performed series superficial transmural myocardial injuries C57BL/6 MRL/MpJ adult mice. At defined time intervals following respective injury ( days 3, 14, 30 60), examined cardiac function using echocardiography, morphology, fluorescence-activated cell sorting for...
Abstract Introduction Hypoxic stress is a feature of rapidly growing thyroid tumours. Cancer progression thought to be driven by small population tumour cells possessing stem cell properties. Hypoxia‐inducible factors (HIFs) are important mediators hypoxia. Both HIF‐1alpha and HIF‐2alpha have been reported expressed in cancers. There evidence that the HIF pathway plays significant role maintenance cancer (CSC). Methodology We isolated CSC from papillary cancer‐derived line (BCPAP) an...
Upon binding with the chemokine CXCL12, receptor CXCR4 has been shown to promote breast cancer progression. This process, however, can be affected by expression of atypical ACKR3. Given ACKR3’s ability form heterodimers CXCR4, we investigated how dual both receptors differed from their lone in terms signalling pathways. We created single and double and/or ACKR3 Chinese hamster ovary (CHO) cell transfectants. ERK Akt phosphorylation after CXCL12 stimulation was assessed correlated...
Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements heart function following treatment have been reported be variable, some showing no response. This discrepancy response remains unresolved. Mesenchymal stem cells (MSCs) highlighted as regenerative tool these display both immunomodulatory and proregenerative activities. The purpose this study was derive MSC population provide an alternative/support current...
Aim: To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings. Materials & methods: Cells were isolated rings cultured in a cell (MSC)-selective media examined for differentiation, phenotyping characterization. Results: LMSCs capable of trilineage adhered to tissue culture plastic, expressed HLA class I surface antigens associated with MSC while having no/low expression II negative hematopoietic lineage markers. They CXCL12-mediated cellular migration....