A5006198474- Cardiac Valve Diseases and Treatments
- Cardiac and Coronary Surgery Techniques
- Telomeres, Telomerase, and Senescence
- Aortic Disease and Treatment Approaches
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiac Structural Anomalies and Repair
- Infective Endocarditis Diagnosis and Management
- Kawasaki Disease and Coronary Complications
- Cardiac pacing and defibrillation studies
- Genetics, Aging, and Longevity in Model Organisms
- Cardiac Ischemia and Reperfusion
- Atherosclerosis and Cardiovascular Diseases
- Trauma Management and Diagnosis
- Congenital Heart Disease Studies
- Hair Growth and Disorders
- Cardiac Arrhythmias and Treatments
- Wound Healing and Treatments
- Cardiac tumors and thrombi
- Coronary Artery Anomalies
- Protease and Inhibitor Mechanisms
- Mitochondrial Function and Pathology
- Cardiovascular Effects of Exercise
Newcastle University
2013-2024
South Tees Hospitals NHS Foundation Trust
2012-2024
National Trust
2024
University of Newcastle Australia
2023
James Cook University Hospital
2012-2022
Centre for Life
2012-2022
Walter Reed National Military Medical Center
2012
National Health Service
2012
Durham University
2008-2011
The University of Texas Southwestern Medical Center
2000-2008
à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Abstract Cardiovascular disease is the leading cause of death in individuals over 60 years old. Aging associated with an increased prevalence coronary artery and a poorer prognosis following acute myocardial infarction (MI). With age, senescent cells accumulate tissues, including heart, contribute to age‐related pathologies. However, role senescence recovery MI has not been investigated. In this study, we demonstrate that treatment aged mice senolytic drug, navitoclax, eliminates...
The novel G-protein, Gh/tissue transglutaminase (TGase II), has both guanosine triphosphatase and Ca2+-activated activity been implicated in a number of processes including signal transduction, apoptosis, bone ossification, wound healing, cell adhesion spreading. To determine the role Gh vivo, Cre/loxPsite-specific recombinase system was used to develop mouse line which its expression ubiquitously inactivated. Despite absence lack intracellular TGase that not compensated by other TGases,...
Abstract A key component of cardiac ischemia–reperfusion injury (IRI) is the increased generation reactive oxygen species, leading to enhanced inflammation and tissue dysfunction in patients following intervention for myocardial infarction. In this study, we hypothesized that oxidative stress, due ischemia–reperfusion, induces senescence which contributes pathophysiology IRI. We demonstrate IRI cellular both cardiomyocytes interstitial cell populations treatment with senolytic drug...
Abstract Myocardial infarction is a leading cause of morbidity and mortality. While reperfusion now standard therapy, pathological remodelling to heart failure remains clinical problem. Cellular senescence has been shown contribute disease pathophysiology treatment with the senolytic navitoclax attenuates inflammation, reduces adverse myocardial results in improved functional recovery. However, it unclear which senescent cell populations these processes. To identify whether cardiomyocytes...
Activation of the α 1A -adrenergic receptor (α -AR)/Gq pathway has been implicated as a critical trigger for development cardiac hypertrophy. However, direct evidence from in vivo studies is still lacking. To address this issue, transgenic mice with cardiac-targeted overexpression -AR (4- to 170-fold) were generated, using rodent α-myosin heavy chain promoter. Heterozygous animals displayed marked enhancement contractility, evident increases dP/dt max (80%, P <0.0001), /LVP inst (76%,...
While the mammalian heart has low, but functionally significant, levels of telomerase expression, cellular population responsible remains incompletely characterized. This study aimed to identify cell types for cardiac activity in neonatal, adult, and cryoinjured adult hearts using transgenic mice expressing green fluorescent protein (GFP), driven by promoter murine reverse transcriptase (mTert), which is a necessary rate-limiting component telomerase. A rare mTert-GFP-expressing cells was...
Human multipotent skin derived precursor cells (SKPs) are traditionally sourced from dissociated dermal tissues; therefore, donor availability may become limiting. Here we demonstrate that both normal and diseased adult human fibroblasts (DF) pre-cultured in conventional monolayers capable of forming SKPs (termed m-SKPs). Moreover, show these m-SKPs can be passaged cryopreservation original fibroblast monolayer cultures does not reduce m-SKP yield; however, extensive passaging does. Like...
To compare clinical and health economic outcomes after manubrium-limited mini-sternotomy (intervention) conventional median sternotomy (usual care).A single-blind, randomised controlled trial.Single centre UK National Health Service tertiary hospital.Adult patients undergoing aortic valve replacement (AVR) surgery.Intervention was performed using a 5-7 cm midline incision. Usual care incision from the sternal notch to xiphisternum.The primary outcome proportion of who received red cell...
Endogenous regeneration has been demonstrated in the mammalian heart after ischemic injury. However, approximately one-third of cases failure are secondary to nonischemic disease and cardiac these remains relatively unexplored. We, therefore, aimed at quantifying rate new cardiomyocyte formation different stages cardiomyopathy. Six-, 12-, 29-, 44-week-old mdx mice received a 7 day pulse BrdU. Quantification isolated nuclei was undertaken using cytometric analysis exclude nondiploid nuclei....
Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements heart function following treatment have been reported be variable, some showing no response. This discrepancy response remains unresolved. Mesenchymal stem cells (MSCs) highlighted as regenerative tool these display both immunomodulatory and proregenerative activities. The purpose this study was derive MSC population provide an alternative/support current...
Propofol (Diprivan™; AstraZeneca, Wilmington, DE) is a commonly used drug for the induction of general anesthesia in ambulatory setting. With availability new bisulfite-containing generic formulation propofol, questions have arisen regarding its cost effectiveness and safety compared with Diprivan™. Two hundred healthy outpatients were randomly assigned, according to double-blinded protocol, receive either Diprivan™ or propofol 1.5 mg/kg IV as part standardized sequence. Maintenance...
OBJECTIVESMinimally invasive surgical approaches for aortic valve replacement (AVR) are growing in popularity an attempt to decrease morbidity from conventional surgery. We have adopted a technique that divides only the manubrium and spares body of sternum. sought determine whether patients benefit this less-invasive approach.
Aortic valve replacement is one of the most common cardiac surgical procedures performed worldwide. Conventional aortic surgery via a median sternotomy; sternum divided completely from sternal notch to xiphisternum. Minimally invasive replacement, using new technique called manubrium-limited ministernotomy, divides only manubrium 1 cm below manubrio-sternal junction. More than third patients undergoing conventional sternotomy develop clinically significant bleeding requiring post-operative...
A focused review of secondary preventive medication following revascularisation provides an opportunity to ensure optimal use these agents. retrospective analysis our in-house cardiothoracic surgical database was performed identify patients undergoing non-emergency, elective discharged on four medications: aspirin; beta-blockers; ACE-inhibitors and statins. Of 2749 studied, 2302 underwent isolated coronary artery bypass grafting (CABG), mean age 65.5 years (S.D. 9.15). Overall, 2536 (92%)...