A5085615474- RNA modifications and cancer
- Congenital heart defects research
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Congenital Heart Disease Studies
- Plant Molecular Biology Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Tracheal and airway disorders
- Cardiac Valve Diseases and Treatments
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Cardiac Structural Anomalies and Repair
- Genetic Mapping and Diversity in Plants and Animals
- Light effects on plants
- Plant nutrient uptake and metabolism
- Genomic variations and chromosomal abnormalities
- Banana Cultivation and Research
- Chromosomal and Genetic Variations
- Coronary Artery Anomalies
University of Manchester
2015-2024
University of Cambridge
2008-2011
Addenbrooke's Hospital
2009-2011
Papworth Hospital
2011
The Gurdon Institute
2011
University of Malaya
2001-2007
The epigenome refers to marks on the genome, including DNA methylation and histone modifications, that regulate expression of underlying genes. A consistent profile gene changes in end-stage cardiomyopathy led us hypothesize distinct global patterns may also exist.We constructed genome-wide maps histone-3 lysine-36 trimethylation (H3K36me3) enrichment for cardiomyopathic normal human hearts. More than 506 Mb sequences per library were generated by high-throughput sequencing, allowing assign...
Epigenetic mechanisms such as microRNA and histone modification are crucially responsible for dysregulated gene expression in heart failure. In contrast, the role of DNA methylation, another well-characterized epigenetic mark, is unknown. order to examine whether human cardiomyopathy different etiologies connected by a unifying pattern methylation pattern, we undertook profiling with ischaemic idiopathic end-stage cardiomyopathic left ventricular (LV) explants from patients who had undergone...
DNA methylation can regulate gene expression by modulating the interaction between and proteins or protein complexes. Conserved consensus motifs exist across human genome ("predicted transcription factor binding sites": "predicted TFBS") but large majority of these are proven chromatin immunoprecipitation high throughput sequencing (ChIP-seq) not to be biological sites ("empirical TFBS"). We hypothesize that at conserved prevents promiscuous disorderly binding.Using genome-wide maps heart...
Long-range chromosomal interactions bring distal regulatory elements and promoters together to regulate gene expression in biological processes. By performing promoter capture Hi-C (PCHi-C) on human embryonic stem cell-derived cardiomyocytes (hESC-CMs), we show that such are a key mechanism by which enhancers contact their target genes after hESC-CM differentiation from hESCs. We also the interactome of hESC-CMs is associated with quantitative trait loci (eQTLs) cardiac left ventricular...
Abstract Background The epigenomes of healthy and diseased human hearts were recently examined by genome-wide DNA methylation profiling. Repetitive elements, heavily methylated in post-natal tissue, have variable profiles cancer but repetitive elements the heart has never been examined. Results We analyzed from all repeat families myocardial samples, found that satellite significantly hypomethylated end-stage cardiomyopathic relative to normal controls. Satellite are almost always...
Genome-wide maps of DNA regulatory elements and their interaction with transcription factors may form a framework for understanding circuits gene expression control in human disease, but how these networks, comprising DNA-binding proteins, complexes, interact modulate remains largely unknown.Using microRNA-21 (mir-21), which is an example genes that are regulated heart failure, we performed chromatin immunoprecipitation (ChIP) assays to determine the occupancy at this genetic locus. Tissue...
Abstract Cross‐talk between the two transcription factors, p53 and hypoxia inducible factor 1α (HIF1A), is important in different pathophysiological conditions (Hammond Giaccia, 2006, Clin Cancer Res 12:5007–5009) such as transition from myocardial hypertrophy to cardiac dilatation heart failure. In that context, induces HIF1A degradation which turn provokes compensatory thinning chamber (Sano et al., 2007, Nature 446:444–448). order investigate mechanism of p53‐induced degradation, we used...
Abstract The frequency and association of polymorphic Alu insertions (POALINs) with human leucocyte antigen (HLA) class I genes within the genomic region major histocompatibility complex (MHC) have been reported previously for three populations: Australian Caucasian, Japanese north‐eastern Thai populations. Here, we report on individual insertion five POALINs MHC region, their HLA‐A HLA‐B associations, POALIN haplotype frequencies HLA‐A/POALIN four‐loci in Malaysian Chinese population....
An Arabidopsis mutant showing an altered ability to green on illumination after extended periods of darkness has been isolated in a screen for genomes uncoupled (gun) mutants. Following 24 h, 10-day-old dark-grown seedlings accumulated five times more chlorophyll than wild-type and this was correlated with differences plastid morphology observed by transmission electron microscopy. The named greening 1 (ged1). Microarray analysis showed much lower amounts transcripts genes encoding seed...
Abstract Disclosure: N.S. Mohd Rizam: None. F.A. Pauzi: M. Choy: S. Williams: A. Aminuddin: F. Abdul Latif: Syed Mohammed Nazri: Mustangin: G. Tan: X. Wu: E. Ng: K. Laycock: N. Sukor: A.B. Nasruddin: M.J. Brown: B. Keavney: E.A. Azizan: Primary aldosteronism (PA) is a common curable cause of hypertension (5-10% cases) yet markedly underdiagnosed. Heredity contributes 25-64% inter-individual blood pressure variation. Genome-wide association studies have identified proportion these genetic...
Abstract The genome-wide promoter interactome is primarily maintained and regulated by architectural proteins such as CTCF cohesin. However, some studies suggest a role for non-coding RNAs (ncRNAs) in this process. We aimed to characterise the regulatory of RNA-mediated interactions control gene expression. integrated datasets RNA-chromatin promoter-genome human embryonic stem cells (hESCs) identify putative interactions. discovered that sites were enriched RNA-PIRs (promoter interacting...
then moved to the UK further her clinical training in general medicine, gastroenterology and hepatology
This article presents data concerning STX18-AS1, a long noncoding RNA gene identified from Genome-wide association study of Atrial Septal Defect (ASD). The describes its expression patterns in human tissues and functions regulating cardiomyocyte differentiation vitro. STX18-AS1 is lncRNA with higher abundance developing tissues, including hearts. Its transcription distribution within the embryonic hearts during key heart septation stages supports STX18-AS1's risk SNPs for ASD. CRISPR stem...
Abstract Eumusa series of banana contains the majority commercially important cultivars, which are interspecific hybrids two wild species bananas, Musa acuminata Colla (AA Group) and balbisiana (BB Group). Most cultivars diploids or triploids. Occurrences ‘laggard’ ‘bridge’ during anaphase were considered as aberrant. Aberrant cells observed in root tip (natural tissue cultured materials), M. thecommon local cultivars: Pisang Mas Group), Berangan (AAA Rastali (AAB Raja Awak (ABB Abu Nipah...
<h3>Introduction</h3> Cardiovascular disease is the leading cause of death worldwide and contributes to a large proportion global health system spending. Furthermore, ten-year mortality rates for common cardiovascular diseases such as heart failure remain unchanged over last few decades despite recent advances in pharmacological treatments. Here we aim investigate mechanism gene expression known genes linked Promotor interacting regions (PIR) are distal regulatory genomic DNA that control...