A5017865455- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Systemic Lupus Erythematosus Research
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- Atherosclerosis and Cardiovascular Diseases
- Chemokine receptors and signaling
- Lymphoma Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Salivary Gland Disorders and Functions
- Chronic Lymphocytic Leukemia Research
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- IL-33, ST2, and ILC Pathways
- interferon and immune responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Retinal Diseases and Treatments
Monash University
2013-2024
The University of Melbourne
2015-2024
The University of Queensland
2022-2024
QIMR Berghofer Medical Research Institute
2020-2024
University of Lausanne
1999-2023
Weatherford College
2023
Australian Regenerative Medicine Institute
2012-2023
Royal Brisbane and Women's Hospital
2023
Peter Doherty Institute
2017-2022
RMIT University
2015-2019
The cause of many autoimmune and inflammatory diseases is unresolved, although dysregulated production tumor necrosis factor (TNF) family members appears to be important in cases. BAFF, a new member the TNF family, binds B cells costimulates their growth vitro. Mice transgenic for BAFF have vastly increased numbers mature effector T cells, develop autoimmune-like manifestations such as presence high levels rheumatoid factors, circulating immune complexes, anti–DNA autoantibodies,...
Members of the tumor necrosis factor (TNF) family induce pleiotropic biological responses, including cell growth, differentiation, and even death. Here we describe a novel member TNF family, designated BAFF (for B activating belonging to family), which is expressed by T cells dendritic cells. Human was mapped chromosome 13q32-34. Membrane-bound processed secreted through action protease whose specificity matches that furin proprotein convertases. The expression receptor appeared be...
BAFF (BLyS, TALL-1, THANK, zTNF4) is a member of the TNF superfamily that specifically regulates B lymphocyte proliferation and survival. Mice transgenic (Tg) for develop an autoimmune condition similar to systemic lupus erythematosus. We now demonstrate Tg mice, as they age, secondary pathology reminiscent Sjögren's syndrome (SS), which manifested by severe sialadenitis, decreased saliva production, destruction submaxillary glands. In humans, SS also correlates with elevated levels...
B cell maturation is a very selective process that requires finely tuned differentiation and survival signals. activation factor from the TNF family (BAFF) member binds to cells potentiates receptor (BCR)-mediated proliferation. A role for BAFF in was suggested by observation of reduced peripheral numbers mice treated with reagents blocking BAFF, high Bcl-2 levels detected transgenic (Tg) mice. We tested vitro effect on lymphocytes derived primary secondary lymphoid organs. induced subset...
BAFF (BLyS, TALL-1, THANK, zTNF4) is a member of the TNF superfamily that specifically regulates B lymphocyte proliferation and survival. Mice transgenic (Tg) for develop an autoimmune condition similar to systemic lupus erythematosus. We now demonstrate Tg mice, as they age, secondary pathology reminiscent Sjögren’s syndrome (SS), which manifested by severe sialadenitis, decreased saliva production, destruction submaxillary glands. In humans, SS also correlates with elevated levels...
Chemotactic cytokines (chemokines) attract immune cells, although their original evolutionary role may relate more closely with embryonic development. We noted differential expression of the chemokine receptor CXCR7 (RDC-1) on marginal zone B a cell type associated autoimmune diseases. generated Cxcr7 −/− mice but found that deficiency had little effect composition. However, most died at birth ventricular septal defects and semilunar heart valve malformation. Conditional deletion in...
Dendritic cell (DC) activation by nucleic acid–containing immunoglobulin (Ig)G complexes has been implicated in systemic lupus erythematosus (SLE) pathogenesis. However, the mechanisms responsible for and subsequent disease induction are not completely understood. Here we show that murine DCs much more effectively activated immune contain IgG bound to chromatin than foreign protein. Activation these occurs two distinct pathways. One pathway involves dual engagement of Fc receptor FcγRIII...
Abstract BAFF (B cell-activating factor belonging to the TNF family) is a cell survival and maturation for B cells, overproduction of associated with systemic autoimmune disease. binds three receptors, BAFF-R, transmembrane activator calcium modulator cyclophilin ligand interactor (TACI), Ag (BCMA). Using specific mAbs, BAFF-R was found be predominant receptor expressed on peripheral in both humans mice, antagonist mAbs blocked BAFF-mediated costimulation anti-μ responses. The other...
The generation of Ig-secreting cells (ISCs) from memory B requires interactions between antigen-specific (Ag-specific) cells, T and dendritic cells. This process must be strictly regulated to ensure sufficient humoral immunity while avoiding production pathogenic autoantibodies. BAFF, a member the TNF family, is key regulator cell homeostasis. BAFF exerts its effect by binding three receptors - transmembrane activator CAML interactor (TACI), maturation antigen (BCMA), receptor (BAFF-R). To...
Tumor necrosis factor alpha (TNF-alpha) is a pleiotropic cytokine triggering cell responses through two distinct membrane receptors. Stimulation of leukocyte adhesion to the endothelium one many TNF-alpha activities and explained by upregulation molecules on endothelial surface. Human umbilical vein cells (HUVEC) were isolated, cultured, demonstrated express both TNF receptor types, TNF-R55 TNF-R75. Cell HUVEC was studied using HL60, U937, MOLT-4 lines. activated either TNF-alpha, binding...
For more than a decade, the biological roles and apparent redundancy of cytokines tumor necrosis factor (TNF) lymphotoxin (LT) have been debated. LT alpha exists in its soluble form as homotrimer, which like TNF only binds receptors, TNF-R55 or TNF-R75. The cell surface heteromer beta subunits this complex specifically receptor (LT beta-R). To discriminate functions systems, beta-R-immunoglobulin (Ig) TNF-R-Ig fusion proteins were introduced into embryonic circulation by injecting pregnant...
Although microRNAs (miRNAs) are key regulators of gene expression, little is known their overall persistence in the cell following processing. Characterization such to full appreciation regulatory roles. Accordingly, we measured miRNA decay rates mouse embryonic fibroblasts loss Dicer1 enzymatic activity. The results confirm inherent stability miRNAs, intracellular levels which were mostly affected by division. Using a panel six miRNAs representative global trend decay, establish...
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by the production of autoantibodies. However, underlying cause appears to relate defects in T cell tolerance or help B cells. Transgenic (Tg) mice overexpressing cytokine cell–activating factor tumor necrosis family (BAFF) develop an disorder similar SLE and show impaired altered differentiation. We generated BAFF Tg that were completely deficient cells, and, surprisingly, these developed SLE-like...