A5061832907- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Acute Ischemic Stroke Management
- Telemedicine and Telehealth Implementation
- Cardiac Health and Mental Health
- Pregnancy and preeclampsia studies
- Kruppel-like factors research
- Mobile Health and mHealth Applications
- Developmental Biology and Gene Regulation
- Advanced Fluorescence Microscopy Techniques
- Vascular Anomalies and Treatments
- Medical Imaging and Analysis
- Congenital Heart Disease Studies
- Acute Myocardial Infarction Research
- Non-Invasive Vital Sign Monitoring
- Atrial Fibrillation Management and Outcomes
- Parathyroid Disorders and Treatments
- Heart Rate Variability and Autonomic Control
- Cardiac Arrhythmias and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Social Media in Health Education
- Cardiovascular and exercise physiology
- Antiplatelet Therapy and Cardiovascular Diseases
- Cerebrovascular and Carotid Artery Diseases
University of Sheffield
2016-2025
Health Data Research UK
2022-2025
British Heart Foundation
2022-2025
Sheffield Teaching Hospitals NHS Foundation Trust
2011-2024
Insigneo
2016-2023
Institute of Infection and Immunity
2023
United Nations Industrial Development Organization
2020
Cognitive Neuroimaging Lab
2019
Royal Hallamshire Hospital
2008-2019
Immunité et Cancer
2018
The zebrafish is an outstanding model for intravital imaging of inflammation due to its optical clarity and the ability express fluorescently labelled specific cell types by transgenesis. However, although several transgenic labelling myeloid cells exist, none allow distinction macrophages from neutrophils. This prevents simultaneous examination individual contributions these important leukocyte subtypes during inflammation. We therefore used Bacterial Artificial Chromosome (BAC)...
Rationale: Blood flow–induced shear stress controls endothelial cell (EC) physiology during atherosclerosis via transcriptional mechanisms that are incompletely understood. The mechanosensitive transcription factor TWIST is expressed embryogenesis, but its role in EC responses to and focal unknown. Objective: To investigate whether regulates vascular dysfunction compare function development disease. Methods Results: expression of TWIST1 was studied both developing vasculature the initiation...
Abstract Angiogenesis requires co-ordination of multiple signalling inputs to regulate the behaviour endothelial cells (ECs) as they form vascular networks. Vascular growth factor (VEGF) is essential for angiogenesis and induces downstream pathways including increased cytosolic calcium levels. Here we show that transmembrane protein 33 ( tmem33 ), which has no known function in multicellular organisms, mediate effects VEGF both zebrafish human ECs. We find localises endoplasmic reticulum ECs...
Summary Interleukin-1, the 'gate-keeper' of inflammation, is apical cytokine in a signalling cascade that drives early response to injury or infection. Expression, processing and secretion IL-1 tightly controlled, whilst dysregulated has been implicated number pathologies ranging from atherosclerosis complications Our understanding these processes comes vitro monocytic cell culture models as lines primary isolates where range spectra mechanisms have described. We therefore investigated...
Abstract Venous endothelial cells are molecularly and functionally distinct from their arterial counterparts. Although veins often considered the default state, genetic manipulations can modulate both acquisition loss of venous fate, suggesting that identity is result active transcriptional regulation. However, little known about this process. Here we show BMP signalling controls via ALK3/BMPR1A receptor SMAD1/SMAD5. Perturbations to TGF-β in mice zebrafish aberrant vein formation expression...
A digital twin is a computer-based "virtual" representation of complex system, updated using data from the "real" twin. Digital twins are established in product manufacturing, aviation, and infrastructure attracting significant attention medicine. In medicine, hold great promise to improve prevention cardiovascular diseases enable personalised health care through range Internet Things (IoT) devices which collect patient real-time. However, such new technology often met with many technical,...
Atherosclerosis is initiated at branches and bends of arteries exposed to disturbed blood flow that generates low shear stress. This mechanical environment promotes lesions by inducing endothelial cell (EC) apoptosis dysfunction via mechanisms are incompletely understood. Although transcriptome-based studies have identified multiple shear-responsive genes, most them an unknown function. To address this, we investigated whether zebrafish embryos can be used for functional screening...
Hemodynamic wall shear stress (WSS) exerted on the endothelium by flowing blood determines spatial distribution of atherosclerotic lesions. Disturbed flow (DF) with a low WSS magnitude and reversing direction promotes atherosclerosis regulating endothelial cell (EC) viability function, whereas un-DF which is unidirectional high atheroprotective. Here, we study role EVA1A (eva-1 homolog A), lysosome endoplasmic reticulum-associated protein linked to autophagy apoptosis, in WSS-regulated EC...
Digital technologies are increasingly being implemented in health care to improve the quality and efficiency of for patients. However, rapid adoption over last 5 years has failed adequately consider patient clinician needs, which results ineffective implementation. There is also a lack consideration differences between resulting overgeneralized approaches implementation use digital technologies. This study aimed explore barriers facilitators diagnosis heart disease both patients clinicians,...
Objective— The role of ischemia in collateral vessel development (arteriogenesis) is a contentious issue that cannot be addressed using mammalian models. To investigate this, we developed models arteriogenesis the zebrafish embryo, which gains sufficient oxygenation via diffusion to prevent response arterial occlusion. Methods and Results— We studied gridlock mutant embryos suffer permanently occluded aorta show these restore aortic blood flow by vessels. phenocopied mutants laser-induced...
The contribution of blood flow to angiogenesis is incompletely understood. We examined the effect on Notch signalling in vasculature zebrafish embryos, and whether regulates with constitutively up-regulated hypoxic signalling. Developing (Danio rerio) embryos survive via diffusion absence circulation induced by knockdown cardiac troponin T2 or chemical cessation. increased vascular 48 h post-fertilization old up-regulation ligand dll4. Despite this, patterning intersegmental vessels not...