A5062280457- Developmental Biology and Gene Regulation
- Muscle Physiology and Disorders
- Kruppel-like factors research
- Hedgehog Signaling Pathway Studies
- Wnt/β-catenin signaling in development and cancer
- Atherosclerosis and Cardiovascular Diseases
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Congenital heart defects research
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related gene regulation
- Apelin-related biomedical research
- Congenital Heart Disease Studies
- Pulmonary Hypertension Research and Treatments
- Renal and related cancers
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Autism Spectrum Disorder Research
- Zebrafish Biomedical Research Applications
- Parkinson's Disease Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- 14-3-3 protein interactions
- Hippo pathway signaling and YAP/TAZ
University of Sheffield
2015-2016
Insigneo
2016
KU Leuven
2016
Boston Children's Hospital
2016
Harvard University
2016
Imperial College London
2016
Erasmus University Rotterdam
2016
University of York
2012-2015
Rationale: Blood flow–induced shear stress controls endothelial cell (EC) physiology during atherosclerosis via transcriptional mechanisms that are incompletely understood. The mechanosensitive transcription factor TWIST is expressed embryogenesis, but its role in EC responses to and focal unknown. Objective: To investigate whether regulates vascular dysfunction compare function development disease. Methods Results: expression of TWIST1 was studied both developing vasculature the initiation...
Introduction and Objectives The zinc-finger transcription factor Krϋppel-like 2 (KLF2) transduces blood flow into molecular signals responsible for a wide range of responses within the vasculature. KLF2 maintains healthy, quiescent endothelial phenotype. Previous studies report phenotypes following morpholino antisense oligonucleotide-induced klf2a knockdown in zebrafish. Targeted genome editing is an increasingly applied method functional assessment candidate genes. We therefore generated...
Lin28 family proteins share a unique structure, with both zinc knuckle and cold shock RNA-binding domains, were originally identified as regulators of developmental timing in Caenorhabditis elegans. They have since been implicated pluripotency mammalian stem cells culture. Using Xenopus tropicalis, we undertaken the first analysis effects on early development vertebrate embryo resulting from global inhibition family. The genome contains two Lin28-related genes, lin28a lin28b. is expressed...
Wnt signalling plays essential roles during embryonic development and is known to be mis-regulated in human disease. There are many molecular mechanisms that ensure tight regulation of activity. One such regulator the heparan-sulfate-specific 6-O-endosulfatase Sulf1. Sulf1 acts extracellularly modify structure heparan sulfate chains affect bio-availability ligands. could, therefore, influence formation complexes modulate activation both canonical non-canonical pathways. In this study, we use...
This study describes how the application of evolutionary algorithms (EAs) can be used to motor function in humans with Parkinson's disease (PD) and animal models PD. Human data is obtained using commercially available sensors via a range non-invasive procedures that follow conventional clinical practice. EAs then classify human for uses, including diagnosis monitoring. New results are presented demonstrate also fruit flies without genetic mutations cause by measurements proboscis extension...
This protocol describes a method to visualise ligands distributed across field of cells. The ease expressing exogenous proteins, together with the large size their cells in early embryos, make Xenopus laevis useful model for visualising GFP-tagged ligands. Synthetic mRNAs are efficiently translated after injection into stage and injections can be targeted single cell. When combined lineage tracer such as membrane tethered RFP, injected cell (and its descendants) that producing overexpressed...
This protocol describes a method to visualise ligands distributed across field of cells. The ease expressing exogenous proteins, together with the large size their cells in early embryos, make Xenopus laevis useful model for visualising GFP-tagged ligands. Synthetic mRNAs are efficiently translated after injection into stage and injections can be targeted single cell. When combined lineage tracer such as membrane tethered RFP, injected cell (and its descendants) that producing overexpressed...