A5089702476- Trypanosoma species research and implications
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Toxin Mechanisms and Immunotoxins
- Chromosomal and Genetic Variations
- Transgenic Plants and Applications
- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Eosinophilic Disorders and Syndromes
- Parkinson's Disease Mechanisms and Treatments
- Biochemical and Molecular Research
- Cellular transport and secretion
- Retinal Development and Disorders
- Hedgehog Signaling Pathway Studies
- Mosquito-borne diseases and control
- Bioactive Natural Diterpenoids Research
- Neuroscience and Neuropharmacology Research
- Protein Tyrosine Phosphatases
- Axon Guidance and Neuronal Signaling
- Muscle Physiology and Disorders
- Autism Spectrum Disorder Research
- Epigenetics and DNA Methylation
University of York
2015-2023
University of North Texas
2022
The Gurdon Institute
2012-2016
University of Cambridge
2012-2016
California Institute of Technology
2008-2013
Wellcome Trust
2012
King's College London
2009
Medical Research Council
2009
Broad Center
2008
Piwi-interacting RNAs (piRNA) are small regulatory with essential roles in maintaining genome integrity animals and protists. Most Caenorhabditis elegans piRNAs transcribed from two genomic clusters that likely contain thousands of individual transcription units; however, their biogenesis is not understood. Here we identify characterize prde-1 (piRNA silencing-defective) as the first C. piRNA gene. Analysis provides direct evidence precursors 28- to 29-nucleotide (nt) initiating 2 nt...
To engineer Nicotiana benthamiana to produce novel diterpenoids, we first aimed increase production of the diterpenoid precursor geranylgeranyl pyrophosphate (GGPP) by up-regulation key genes non-mevalonate (MEP) pathway sourced from Arabidopsis thaliana. We used transient expression evaluate combinations eight MEP plus GGPP synthase and a Jatropha curcas casbene (JcCAS) identify an optimal combination for GGPP. AtDXS AtHDR together with AtGGPPS JcCAS gave 410% in compared alone. This was...
Significance Euphorbia peplus , a member of the genus, is rich in jatrophane and ingenane diterpenoids. Using metabolomics-guided transcriptomic approach to gene candidate identification, we have discovered short-chain dehydrogenase involved production lathyrane jolkinol E. We developed virus-induced gene-silencing method that has allowed us demonstrate direct relationship between casbene polycyclic diterpenoids C acts as key branch point intermediate ingenanes jatrophanes. This work...
In a number of Drosophila models genetic Parkinson's disease (PD) flies climb more slowly than wild-type controls. However, this assay does not distinguish effects PD-related genes on gravity sensation, "arousal", central pattern generation leg movements, or muscle. To address problem, we have developed an for the fly proboscis extension response (PER). This is attractive because PER has simple, well-identified reflex neural circuit, in which sucrose sensing neurons activate pair "command...
This study describes how the application of evolutionary algorithms (EAs) can be used to motor function in humans with Parkinson's disease (PD) and animal models PD. Human data is obtained using commercially available sensors via a range non-invasive procedures that follow conventional clinical practice. EAs then classify human for uses, including diagnosis monitoring. New results are presented demonstrate also fruit flies without genetic mutations cause by measurements proboscis extension...
Non-coding RNAs are crucial regulators for a vast array of cellular processes and have been implicated in human disease. These biological represent hitherto untapped resource our fight against In this work we identify small molecule inhibitors non-coding RNA uridylylation pathway. The TUTase family enzymes is important modulating pathways both cancer pathogen systems. We demonstrate that new class drug target can be accessed with traditional discovery techniques. Using the Trypanosoma brucei...
Plants respond to heat stress by producing heat-shock proteins. These are regulated promoters containing regulatory elements, which can be harnessed control protein expression both temporally and spatially. In this study, we designed heat-inducible produce the diterpene casbene in Nicotiana benthamiana, through a multi-step metabolic pathway. To potentially increase gene transcription, coupled elements from Arabidopsis thaliana Hsp101 or Glycine max GmHsp17.3-B promoters, CAAT TATA boxes...