A5105825207- Long-Term Effects of COVID-19
- Genetics, Bioinformatics, and Biomedical Research
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- COVID-19 Clinical Research Studies
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Advanced Biosensing Techniques and Applications
- Acute Lymphoblastic Leukemia research
- Animal Genetics and Reproduction
- Protein purification and stability
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Cancer-related gene regulation
- COVID-19 diagnosis using AI
- Liver Disease Diagnosis and Treatment
- NF-κB Signaling Pathways
- Fungal and yeast genetics research
- Molecular Biology Techniques and Applications
- Diet and metabolism studies
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Insect and Arachnid Ecology and Behavior
- CAR-T cell therapy research
- Insect and Pesticide Research
Max Planck Institute for Molecular Genetics
1998-2022
Alacris (Germany)
2015-2022
Max Planck Society
2003-2005
Uppsala University
2001
Humboldt-Universität zu Berlin
2000
Preface. Foreword. Part I: General Introduction. 1. Basic Principles. 2. Biology in a Nutshell. 3. Mathematics 4. Experimental Techniques II: Standard Models and Approaches Systems Biology. 5. Metabolism. 6. Signal Transduction. 7. Selected Biological Processes. 8. Modeling of Gene Expression. 9. Analysis Expression Data. 10. Evolution Self-organization. 11. Data Integration. 12. What's Next? III: Computer-based Information Retrieval Examination. 13. Databases Tools on the Internet. 14....
GOblet is a comprehensive web server application providing the annotation of anonymous sequence data with Gene Ontology (GO) terms. It uses variety different protein databases (human, murines, invertebrates, plants, sp-trembl) and their respective GO mappings. The user selects appropriate database alignment threshold thereafter submits single or multiple nucleotide sequences. Results are shown in ways, e.g. as survey statistics for main categories all sequences detailed results each that has...
We have developed an off-line coupling of capillary electrophoresis (CE) to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) based on CE fraction collection onto prestructured MALDI sample supports. Analyte carryover and detection sensitivity were investigated using a standard peptide mixture. Low femtomole amounts detected, no noticeable was discovered. The performance the method evaluated with mixture tryptic digests proteins from human fetal brain...
A new strategy for identifying proteins by MALDI-TOF-MS peptide mapping is reported. In contrast to current approaches, the does not rely on a good relative or absolute mass accuracy as criterion that discriminates false positive results. The protein sequence database first searched all match minimum five of submitted masses within maximum expected errors when default externally determined calibration constants are used, instance, ±500 ppm. Typically, this search retrieves many thousand...
We have used oligonucleotide-fingerprinting data on 60,000 cDNA clones from two different mouse embryonic stages to establish a normalized clone set. The set of 5,376 represents clusters and therefore, in almost all cases, genes. inserts the were amplified by PCR spotted glass slides. resulting arrays hybridized with mRNA probes prepared six adult tissues. Expression profiles analyzed hierarchical clustering techniques. chosen radioactive detection because it combines robustness sensitivity...
Secondary infections contribute significantly to covid-19 mortality but driving factors remain poorly understood. Autopsies of 20 cases and 14 controls from the first pandemic wave complemented with microbial cultivation RNA-seq lung tissues enabled description major organ pathologies specification secondary infections. Lethal segregated into two main death causes either dominant diffuse alveolar damage (DAD) or pneumonias. The microbiome in showed a reduced biodiversity increased...
The response of cancer cells to drugs is determined by various factors, including the cells’ mutations and gene expression levels. These factors can be assessed using next-generation sequencing. Their integration with vast prior knowledge on signaling pathways is, however, limited availability mathematical models scalable computational methods. Here, we present a framework for parameterization large-scale mechanistic its application prediction drug cell lines from exome transcriptome...
Abstract Secondary infections contribute significantly to covid-19 mortality but host and microbial factors driving this sequel remain poorly understood. We performed an autopsy study of 20 cases 14 controls from the first pandemic wave. Autopsies combined with cultivation deep RNA sequencing (RNAseq) allowed us define major organ pathologies specify secondary infections. Lethal segregated into two main death causes separating either dominant diffuse alveolar damage (DAD) or lungs. Lung...
Abstract Proteomics ‐ die Analyse des Zusammenspiels aller Proteine eines Organismus‐besteht aus einem ganzen Bündel an Forschungsprojekten. Entsprechend vielfältig sind eingesetzten Techniken. Die Datenflut integrierten Ansätzen, zum Beispiel der Kombination Trennverfahren und Strukturaufklärung, lässt sich nur noch rechnerisch mit den Methoden Bioinformatik bewältigen.
9566 Background: There is a lack of active treatment against mUM. Such “hard-to-treat” tumour might benefit from decisions driven by complete genomic and transcriptomic analysis program. Methods: From 1.3.2016 to 1.12.2017, mUM were included in the prospective TREAT20Plus study subjected CMTA including WGS, WES, RNAseq, cell culture systems biological/pharmacodynamic modelling. Treatment recommendations made molecular board. Results: Twenty six patients (12 F, 14M). Age: 61 (32-80). PS: 0...
Secondary infections contribute significantly to covid-19 mortality but host and microbial factors driving this sequel remain poorly understood. We performed an autopsy study of 20 cases 14 controls from the first pandemic wave. Autopsies combined with cultivation deep RNA sequencing (RNAseq) allowed us define major organ pathologies specify secondary infections. Lethal segregated into two main death causes separating either dominant diffuse alveolar damage (DAD) or lungs. Lung microbiome...