A5078352756- Histone Deacetylase Inhibitors Research
- Peroxisome Proliferator-Activated Receptors
- Protein Degradation and Inhibitors
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- NF-κB Signaling Pathways
- Eicosanoids and Hypertension Pharmacology
- Retinoids in leukemia and cellular processes
- Pharmacogenetics and Drug Metabolism
- Toxic Organic Pollutants Impact
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- HIV/AIDS drug development and treatment
- Inflammatory mediators and NSAID effects
- Acute Myeloid Leukemia Research
- Effects and risks of endocrine disrupting chemicals
- Pharmacological Effects and Toxicity Studies
- Drug Transport and Resistance Mechanisms
- Peptidase Inhibition and Analysis
- Cytokine Signaling Pathways and Interactions
- Carcinogens and Genotoxicity Assessment
- Growth Hormone and Insulin-like Growth Factors
- Protein Tyrosine Phosphatases
- interferon and immune responses
Technical University of Munich
2007-2021
Helmholtz Zentrum München
2008-2021
Inserm
2008
Ludwig-Maximilians-Universität München
2008
Institute of Bioinformatics and Systems Biology
2008
FZI Research Center for Information Technology
1996-2005
Krankenhaus Nordwest
2004
Georg Speyer Haus
2004
Institute of Groundwater Ecology
2004
Heidelberg University
2002
Peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643 have been shown to activate PPAR (peroxisome proliferator-activated receptor), a member of the steroid nuclear receptor superfamily. We cloned cDNA from rat that is homologous mouse [Issemann, I. & Green, S. (1990) Nature (London) 347, 645-650], which encodes 97% similar protein with particularly well-conserved putative ligand-binding domain. To search for physiologically occurring activators, we established...
The rat peroxisome-proliferator-activated receptor (PPAR) was expressed in insect cells and shown to bind a cognate PPAR response element (PPRE) from the acyl-CoA oxidase gene. Upon purification, no longer able DNA, although binding could be restored by addition of cell extracts. We investigated whether retinoid X (RXR) supplement for this accessory activity. RXR alpha cDNA cloned it found that vitro-translated purified facilitated PPRE. Furthermore, an additional activity, which appeared...
Siva Kumar Kolluri, Carsten Weiss, Andrew Koff, and Martin Göttlicher Forschungszentrum Karlsruhe, Institute of Genetics, 76021 Germany; Laboratory Cell Cycle Regulation, Memorial Sloan-Kettering Cancer Center, New York, York 10021 USA
Altered histone deacetylase (HDAC) activity has been identified in several types of cancer. This study was designed to determine the safety and maximum tolerated dose (MTD) valproic acid (VPA) as an HDAC inhibitor cancer patients. Twenty-six pre-treated patients with progressing solid tumours were enrolled dose-escalating three-patient cohorts, starting at a VPA 30 mg kg−1 day−1. administered 1-h infusion daily for 5 consecutive days 21-day cycle. Neurocognitive impairment dominated toxicity...
<h3>Background:</h3> Although histone deacetylase inhibitors (HDACi) are promising cancer therapeutics regulating proliferation, differentiation and apoptosis, molecular pathways engaged by specific HDAC isoenzymes in ill defined. <h3>Results:</h3> In this study we demonstrate that HDAC2 is highly expressed pancreatic ductal adenocarcinoma (PDAC), especially undifferentiated tumours. We show HDAC2, but not HDAC1, confers resistance towards the topoisomerase II inhibitor etoposide PDAC cells....
Hypolipidaemic fatty acid derivatives and polyunsaturated acids decrease concentrations of plasma triacylglycerol by mechanisms that are not fully understood. Because poor susceptibility to beta- and/or omega-oxidation is apparently a determinant the peroxisome proliferating hypolipidaemic capacity derivatives, relative importance activation peroxisome-proliferator-activated receptor alpha (PPARalpha), oxidation synthesis were examined. We have compared effects differentially beta-oxidizable...
Abstract Histone deacetylases (HDAC) reverse the acetylation of histone and nonhistone proteins thereby modulate chromatin structure function proteins. Many tumor cell lines experimental tumors respond to HDAC inhibition. To assess role an individual isoenzyme in physiology development, HDAC2-mutant mice were generated from a gene trap embryonic stem clone. These express catalytically inactive fusion protein NH2-terminal part HDAC2 β-galactosidase, which fails integrate into corepressor...
Gene expression changes during cell differentiation are thought to be coordinated by histone modifications, but still little is known about the role of specific deacetylases (HDACs) in fate decisions vivo . Here we demonstrate that catalytic function HDAC2 required adult, not embryonic neurogenesis. While brain development and adult stem were normal upon conditional deletion or mice lacking activity HDAC2, neurons derived from both zones neurogenesis die at a maturation stage. This phenotype...
Glucocorticoid receptor (GR)-mediated transrepression of the transcription factors AP-1 and NF-κB, responsible for most anti-inflammatory effects glucocorticoids, is initiated by tethering GR to promoters target genes. We report that this mediated a nuclear isoform focal adhesion LIM domain protein Trip6. Trip6 functions as coactivator both NF-κB. As shown chromatin immunoprecipitation, recruited genes together with or In presence joins complex. Reducing level RNA interference abolishing its...
ABSTRACT The extracellular matrix component hyaluronan is believed to play important roles in various processes of organogenesis, cell migration and cancer. Recognition binding mediated by surface receptors. Three them, CD44, ICAM-1 RHAMM (receptor for hyaluronic acid motility), have been identified. A cDNA clone designated turned out possess transforming capacity. Based on this published sequence, we isolated the complete murine gene. comprises an open reading frame 2.3 kb encodes a 95 kDa...