A5049543124- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Retinal and Optic Conditions
- Antimicrobial Peptides and Activities
- Retinal and Macular Surgery
- Ocular Infections and Treatments
- Ocular Oncology and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Connective tissue disorders research
- Biochemical and Structural Characterization
- Intraocular Surgery and Lenses
- Immune Response and Inflammation
- Nanoparticles: synthesis and applications
- Cell Adhesion Molecules Research
- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Retinal Imaging and Analysis
- Veterinary Oncology Research
- Proteoglycans and glycosaminoglycans research
- Nasolacrimal Duct Obstruction Treatments
- Cutaneous Melanoma Detection and Management
University of California, Davis
2016-2025
University of Ottawa
2006-2023
Ottawa Hospital
2005-2023
Ottawa Hospital Research Institute
2010-2023
Veterinary Medical Teaching Hospital
2015-2021
University of Wisconsin–Madison
1993-2021
Roche (Switzerland)
2016-2018
Roche (China)
2018
California Institute for Regenerative Medicine
2011
Stanford University
2011
Early prediction of human clearance is often challenging, in particular for the growing number low-clearance compounds. Long-term vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved predictions. Here, cell line HepG2, iPSC-derived hepatocytes (iCell®), stem HepaRG™, hepatocyte co-cultures (HμREL™ HepatoPac®) were compared to primary suspension cultures with respect their key metabolic activities. Similar activities found long-term HμREL™,...
The cornea, as a dynamic and responsive tissue, constantly interacts with mechanical forces in order to maintain its structural integrity, barrier function, transparency refractive power. Cells within the cornea sense respond various that fundamentally regulate their morphology fate development, homeostasis pathophysiology. Corneal cells also dynamically extracellular matrix (ECM) ensuing cell-ECM crosstalk serves signaling reservoir providing biophysical biochemical cues corneal cells. Here...
The t(1;19) translocation yields a fusion between E2A and PBX1 genes occurs in 5% of acute lymphoblastic leukemia children adults. We used chromosomal translocations Ig heavy chain ( IGH )/T cell antigen receptor TCR ) rearrangements to develop an understanding the etiology natural history this subtype leukemia. sequenced genomic 22 preB leukemias two lines. prenatal origin was assessed 15 pediatric patients by screening for clonotypic - neonatal blood spots, or Guthrie cards, obtained from...
BackgroundRetinitis pigmentosa (RP) is a blinding genetic disorder that caused by the death of photoreceptors in outer nuclear layer retina. To date, 39 different loci have been associated with disease, and 28 mutated genes identified. Despite complexity underlying basis for RP, final common pathway photoreceptor cell via apoptosis.Methodology/Principal FindingsIn this study, P23H S334ter rhodopsin transgenic rat models RP were used to test neuroprotective effects anti-apoptotic gene...
Marfan syndrome (MFS) is a heritable connective tissue disorder caused by mutations in the gene coding for FIBRILLIN-1 (FBN1), an extracellular matrix protein. MFS inherited as autosomal dominant trait and displays major manifestations ocular, skeletal, cardiovascular systems. Here we report molecular phenotypic profiles of skeletogenesis tissues differentiated from human embryonic stem cells induced pluripotent that carry mutation FBN1. We demonstrate that, biological consequence activation...
Abstract Purpose Corneal dysmorphologies (CDs) are typically classified as either regressive degenerative corneal dystrophies (CDtrs) or defective growth and differentiation-driven dysplasias (CDyps). Both eye disorders have multifactorial etiologies. While previous work has elucidated many aspects of CDs, such presenting symptoms, epidemiology, pathophysiology, the genetic mechanisms remain incompletely understood. The purpose this study was to analyze phenotype data from 8,707 knockout...
The skin functions as more than a physical barrier to infection. Epithelial cells of the can synthesize antimicrobial peptides, including defensins, which exhibit direct activity. Here we characterize expression pattern, genetic variation and activity major β-defensin expressed in canine skin, 103 (CBD103). gene encoding CBD103 exhibits two forms polymorphism: common 3-basepair deletion allele copy-number variation. Golden retrievers Labrador were only breeds that encoded variant...
Abstract Despite advances in next generation sequencing technologies, determining the genetic basis of ocular disease remains a major challenge due to limited access and prohibitive cost human forward genetics. Thus, less than 4,000 genes currently have available phenotype information for any organ system. Here we report ophthalmic findings from International Mouse Phenotyping Consortium, large-scale functional screen with goal generating phenotyping null mutant every mouse gene. Of 4364...
Purpose: The purpose of this study was to determine the influence mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on surface properties cells, such as wettability, contact angle, and heterogeneity. Methods: hTCEpi cells were cultured confluence serum-free medium. medium then replaced by stratification induce biosynthesis. profile analyzed using quantitative PCR Western blotting. Contact angles measured a two-immiscible liquid method, angle hysteresis evaluated...
To evaluate the neuroprotective effects of adenoassociated virus delivery XIAP in N-methyl-N-nitrosourea (MNU)-induced retinal degeneration Sprague-Dawley rats.Sprague-Dawley rats were injected subretinally with recombinant (rAAV) encoding either or green fluorescent protein (GFP; injection control). Six weeks after injection, animals received an intraperitoneal MNU, a DNA methylating agent, at dose 60 mg/kg. Electroretinograms (ERGs) recorded 0, 24, 48 and 72 hours 1 week MNU. The killed...
Mucosal and disseminated candidiasis in gnotobiotic SCID mice Get access E. Balish, Balish Search for other works by this author on: Oxford Academic PubMed Google Scholar J. Jensen, Jensen T. Warner, Warner Brekke, Brekke B. Leonard Journal of Medical Veterinary Mycology, Volume 31, Issue 2, March 1993, Pages 143–154, https://doi.org/10.1080/02681219380000161 Published: 01 1993 Article history Accepted: 28 October 1992
The purpose of this study was to quantify the frequency and severity ocular abnormalities affecting wild-type C57BL/6N mice, most common strain used worldwide for creation single-gene knockouts.A total 2773 animals (5546 eyes) were examined at one colony UC Davis in three more colonies Institut Clinique de la Souris Strasbourg, France. Mice 15 16 weeks postnatal age by performing anterior segment biomicroscopy, posterior examination indirect ophthalmoscopy, intraocular pressure measurement,...
Purpose: We sought to define the role of Wwtr1 in murine ocular structure and function determine mechanotransduction Fuchs' endothelial corneal dystrophy (FECD), with emphasis on interactions between cells (CEnCs) Descemet's membrane (DM). Methods: A deficient mouse colony was established, advanced imaging, atomic force microscope (AFM), histology/immunofluorescence were performed. Corneal wound healing assessed using cryoinjury phototherapeutic keratectomy mice. Expression WWTR1/TAZ...