The combination of stem cell therapy with a supportive scaffold is promising approach to improving cardiac tissue engineering. Stem can be used repair nonfunctioning heart and achieve myocardial regeneration, materials utilized in order successfully deliver support cells vivo. Current research describes passive materials; here an electroactive that provides electrical, mechanical, topographical cues induced human pluripotent (iPS) presented. poly(lactic-co-glycolic acid) fiber coated...

The implant-host interface is a critical element in guiding tissue or organ regeneration. We previously developed hydrogels comprising interpenetrating networks of recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine (RHCIII-MPC) as substitutes for the corneal extracellular matrix that promote endogenous regeneration tissue. To render them functional clinical application, we have now optimized their composition thereby enhanced mechanical properties. demonstrated...

Biomaterials designed to replace the diseased cornea could be used treat corneal blindness where human donor tissue is in short supply, but challenges are integration of biomaterials with host and cells, avoiding a rapid material degradation maintaining transparency. Additionally, implantation surgery often triggers an aggressive wound healing response that can lead thinning opacity. Here, we report collagen-based hydrogel transparency mechanical properties suitable for replacing substantial...

In cases of blinding disease or trauma, hydrogels have been proposed as scaffolds for corneal regeneration and vehicles ocular drug delivery. Restoration transparency, augmenting a thin cornea postoperative delivery are particularly challenging in resource-limited regions where availability patient compliance may be suboptimal. Here, we report bioengineered hydrogel based on porcine skin collagen an alternative to human donor tissue applications long-term stability the is required. The...

Cardiac tissue engineering via the use of stem cells is future for repairing impaired heart function that results from a myocardial infarction. Developing an optimised platform to support vital realising this, and through utilising new 'smart' materials such as conductive polymers we can provide multi-pronged approach supporting stimulating engineered surface properties, electrical, electromechanical stimulation. Here present fundamental study on viability cardiac progenitor polymer...

Severe shortage of donor corneas for transplantation, particularly in developing countries, has prompted the advancement bioengineered tissue alternatives. Bioengineered that can withstand transplantation while maintaining transparency and compatibility with host cells, are additionally amenable to standardized low-cost mass production sought. In this study, a porcine construct (BPC) was developed function as biodegradable scaffold promote corneal stromal regeneration by cells. Using...

// Artur Cieślar-Pobuda 1, 2 , Mehrdad Rafat 3 Viktoria Knoflach 4 Magdalena Skonieczna 2, 5 Andrzej Hudecki 6 Małecki 7 Elżbieta Urasińska 8 Seaid Ghavami 9 Marek J. Łos 3, 1 Stem Cell Group, Nordic EMBL Partnership, Centre for Molecular Medicine Norway (NCMM), University of Oslo, Institute Automatic Control, Silesian Technology, Gliwice, Poland LinkoCare Life Sciences AB, Mjärdevi Science Park, Linköping, Sweden Unit Neurobiology, Department Medical Biochemistry & Biophysics, Karolinska...

The human gyrovirus derived protein Apoptin (HGV-Apoptin) a homologue of the chicken anemia virus (CAV-Apoptin), with high cancer cells selective toxicity, triggers apoptosis selectively in cells. In this paper, we show that HGV-Apoptin acts independently from death receptor pathway as it induces similar rates Jurkat deficient either FADD (fas-associated domain) function or caspase-8 (key players extrinsic pathway) and their parental clones. via activation mitochondrial intrinsic pathway. It...

Cardiovascular diseases, including myocardial infarction, are the cause of significant morbidity and mortality globally. Tissue engineering is a key emerging treatment method for supporting repairing cardiac scar tissue caused by infarction. Creating cell supportive scaffolds that can be directly implanted on infarct an attractive solution. Hydrogels made collagen highly biocompatible materials molded into range shapes suitable patch applications. The addition mechanically reinforcing...

Abstract Hollow fibers were spun from a solution of surface‐modifying macromolecule blended polyethersulfone in dimethyl acetamide by using dry‐wet spinning method at different air gaps and room temperature. The gap was varied 10 to 90 cm. ultrafiltration performance hollow studied aqueous solutions polyethylene glycols oxides molecular weights. Significant difference surface morphology between the inner outer observed atomic force microscopy (AFM). Similar results obtained contact angle...

Abstract Fluorescent compounds capable of staining cells selectively without affecting their viability are gaining importance in biology and medicine. Recently, a new family optical dyes, denoted luminescent conjugated oligothiophenes (LCOs), has emerged as an interesting class highly emissive molecules for studying various biological phenomena. Properly functionalized LCOs have been utilized selective identification disease‐associated protein aggregates detection distinct cells. Herein, we...

Purpose/Aim: Evaluating the suitability of bioengineered collagen sheets and human anterior lens capsules (HALCs) as carriers for cultivated porcine corneal endothelial cells (pCECs) in vitro assessment cell-carrier tissue-engineered grafts Descemet membrane keratoplasty (DMEK).pCECs were isolated, cultured up to P2 seeded onto LinkCell™ matrices 20 µm (LK20) or 100 (LK100) thickness, on HALC. During expansion, pCEC viability morphology assessed by light microscopy. ZO-1 Na+/K+-ATPase...

Abstract The internal and external curved surfaces of polysulfone hollow fiber membranes were characterized by atomic force microscopy (AFM), contact angle measurement (CAM), scanning electron (SEM) with the aim improving membrane surface properties for blood compatibility. Novel approaches applied to evaluate a number properties, including roughness, pore size, nodule wettability fibers. CAM studies carried out directly observing liquid meniscus at Observation became possible using an...

Abstract Our objective was to develop collagen‐based hydrogels as tissue substitutes for corneal transplantation. The design of the full‐thickness grafts includes prevention cell migration onto posterior surface implants, using a plasma‐assisted modification technique. Briefly, hydrogel materials were subjected ammonia plasma functionalization followed by grafting alginate macromolecules target surface. treated surfaces showed observable decreases in endothelial attachment. decrease...

Purpose: Our objective was to evaluate promotion of tissue regeneration by extracellular matrix (ECM) mimics, using corneal implantation as a model system. Methods: Carbodiimide cross-linked porcine type I collagen molded into appropriate dimensions serve substitutes for natural ECM. These were implanted corneas mini-pigs after removal the host tissue, and tracked over 12 months, clinical examination, slit-lamp biomicroscopy, in vivo confocal microscopy, topography, esthesiometry....

Therapeutic induction of blood vessel growth (angiogenesis) in ischemic tissues holds great potential for treatment myocardial infarction and stroke. Achieving sustained angiogenesis vascular maturation has, however, been highly challenging. Here, we demonstrate that alginate:collagen hydrogels containing therapeutic, pro-angiogenic FGF-2, formulated as microspheres, is a promising clinically relevant vehicle therapeutic angiogenesis. By titrating the amount readily dissolvable degradable...

By-pass surgery and heart transplantation are traditionally used to restore the heart's functionality after a myocardial Infarction (MI or attack) that results in scar tissue formation impaired cardiac function. However, both procedures associated with serious post-surgical complications. Therefore, new strategies help re-establish necessary. Tissue engineering stem cell therapy promising approaches being explored for treatment of MI. The niche is extremely important proliferation...

Abstract Argon plasma treatment enhanced the attachment of epithelial cells to a collagen‐based artificial cornea crosslinked using glutaraldehyde (GA) and glutaraldehyde‐polyethylene oxide dialdehyde (GA‐PEODA) systems. The epithelialization untreated treated surfaces was evaluated by seeding growth human corneal cells. Characterization polymer surface properties such as hydrophilicity roughness also made contact angle measurement atomic force microscopy, respectively. Contact analysis...