A5022582566- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Herpesvirus Infections and Treatments
- Graphene and Nanomaterials Applications
- Tissue Engineering and Regenerative Medicine
- Advanced Sensor and Energy Harvesting Materials
- Pancreatic function and diabetes
- Marine Biology and Environmental Chemistry
- Seaweed-derived Bioactive Compounds
- Silk-based biomaterials and applications
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Collagen: Extraction and Characterization
- Additive Manufacturing and 3D Printing Technologies
- Nanoparticles: synthesis and applications
- Antimicrobial Peptides and Activities
- RNA Research and Splicing
- RNA modifications and cancer
- Conducting polymers and applications
- Intraocular Surgery and Lenses
- RNA and protein synthesis mechanisms
- Anesthesia and Neurotoxicity Research
Université de Montréal
2020-2024
Linköping University
2014-2023
Hôpital Maisonneuve-Rosemont
2020-2023
Centre Hospitalier de l’Université de Montréal
2022
Uppsala University
2022
Karolinska Institutet
2016
LiQD Cornea is a liquid in situ gelling, pro-regeneration hydrogel for treating corneal blindness and repairing perforations.
The implant-host interface is a critical element in guiding tissue or organ regeneration. We previously developed hydrogels comprising interpenetrating networks of recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine (RHCIII-MPC) as substitutes for the corneal extracellular matrix that promote endogenous regeneration tissue. To render them functional clinical application, we have now optimized their composition thereby enhanced mechanical properties. demonstrated...
Abstract Pathophysiologic inflammation, e.g., from HSV-1 viral infection, can cause tissue destruction resulting in ulceration, perforation, and ultimately blindness. We developed an injectable Cornea-in-a-Syringe (CIS) sealant-filler to treat damaged corneas. CIS comprises linear carboxylated polymers of inflammation-suppressing 2-methacryloyloxyethyl phosphorylcholine, regeneration-promoting collagen-like peptide, adhesive collagen-citrate glue. also incorporated GF19, a modified...
Abstract Purpose To determine feasibility of plant-derived recombinant human collagen type I (RHCI) for use in corneal regenerative implants Methods RHCI was crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form hydrogels. Application shear force liquid crystalline aligned the fibrils. Both random hydrogels were evaluated mechanical optical properties, as well vitro biocompatibility. Further evaluation performed vivo by subcutaneous...
Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential limited by rapid loss introduced therapeutic cells before their effects can be exploited, due in part to anoikis, and adverse environments often found pathologic tissues that have been grafted into. Encapsulation individual has proposed as a means increasing cell viability. In this study, we developed facile, high throughput method for creating temperature responsive microcapsules...
Our aim was to determine the effect of a surgical technique on biomaterial implant performance, specifically graft retention.Twelve mini pigs were implanted with cell-free, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) cross-linked recombinant human collagen type III (RHCIII) hydrogels as substitutes for donor corneal allografts using overlying sutures or without amniotic membrane (HAM) versus interrupted HAM. The effects retention method compared well...
Severe HSV-1 infection can cause blindness due to tissue damage from severe inflammation. Due the high risk of graft failure in HSV-1-infected individuals, cornea transplantation restore vision is often contraindicated. We tested capacity for cell-free biosynthetic implants made recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine (RHCIII-MPC) suppress inflammation promote regeneration damaged corneas. To block viral reactivation, we incorporated silica dioxide...
Sterilization of biodegradable, collagen-based implants is challenging as irradiation sterilization methods can alter their mechanical properties. Electron beam (EB) a terminal method that has been used for biologically-derived implants. Here, recombinant human collagen type III-phosphorylcholine (RHCIII-MPC) hydrogels were irradiated with EB doses 17, 19, or 21 kGy and subsequent biocompatibility ability to promote regeneration in rabbit corneas was evaluated. Unirradiated stored 1%...
Background: Regeneration of nerves or nerve bundles is problematic mainly due to issues finding their target tissues. A very clear example this the lack treatments for traumatic injury optic nerve, something that associated with surgery trauma skull. Nerve guides have been used support better part last century, unfortunately clinical improvements in patients receiving sort treatment poor. Large type are needed make a viable solution repair. It has shown electrostimulation cells on conductive...
Background: The sterilization of corneal implants composed carbodiimide crosslinked recombinant human collagen type III (RHCIII) and phosphorylcholine polymers (RHCIII-MPC) is constrained by the biochemical properties RHCIII. Early trials used 1% chloroform in 0.1 M phosphate buffered saline (C-PBS), but require a stringent wash procedure with antibiotics to remove chloroform. Irradiation gamma or electron-beam (e-beam) allows chemical-free method, may result crosslinking denaturation. Here,...