A5078408394- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Connective tissue disorders research
- Proteoglycans and glycosaminoglycans research
- Protease and Inhibitor Mechanisms
- Glaucoma and retinal disorders
- Periodontal Regeneration and Treatments
- Ocular Infections and Treatments
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Paleontology and Stratigraphy of Fossils
- Collagen: Extraction and Characterization
- Aortic aneurysm repair treatments
- Bone Tissue Engineering Materials
- Cephalopods and Marine Biology
- Legionella and Acanthamoeba research
- melanin and skin pigmentation
- Photoreceptor and optogenetics research
- 3D Printing in Biomedical Research
- Aortic Disease and Treatment Approaches
- Optical Polarization and Ellipsometry
- Effects of Vibration on Health
- Electrohydrodynamics and Fluid Dynamics
- Skin Protection and Aging
Cardiff University
2016-2025
University of Oxford
2009
LiQD Cornea is a liquid in situ gelling, pro-regeneration hydrogel for treating corneal blindness and repairing perforations.
The severe worldwide shortage of donor organs, and pathologies placing patients at high risk for rejecting conventional cornea transplantation, have left many corneal blind untreated. Following successful pre-clinical evaluation in mini-pigs, we tested a biomaterials-enabled pro-regeneration strategy to restore integrity an open-label observational study six patients. Cell-free implants comprising recombinant human collagen phosphorylcholine were grafted by anterior lamellar keratoplasty...
The cornea is the main refracting lens in eye. As part of outer tunic it has to be resilient, a property conferred by organisation constituent collagen. It also sufficiently elastic regain its exact shape when deformed, order not distort retinal image. basis this elasticity fully understood. purpose study was characterise three dimensions arrangement and distribution fibers human corneal stroma, using serial block face scanning electron microscopy. We have demonstrated that there exists...
Short collagen-like peptides (CLPs) are being proposed as alternatives to full-length collagen for use in tissue engineering, on their own soft hydrogels, or conjugated synthetic polymer mechanical strength. However, despite intended clinical use, little is known about safety and efficacy, mechanism of action degree similarity the counterparts they mimic. Here, we show functional equivalence a CLP polyethylene glycol (CLP-PEG) recombinant human vitro promoting stable regeneration corneal...
The optical and biomechanical properties of the cornea are largely governed by collagen-rich stroma, a layer that represents approximately 90% total thickness. Within specific arrangement superimposed lamellae provides tissue with tensile strength, whilst spatial individual collagen fibrils within confers transparency. In keratoconus, this precise stromal is lost, resulting in ectasia visual impairment. normal cornea, we previously characterised three-dimensional an elastic fiber network...
Matrix assembly and homeostasis in collagen-rich tissues are mediated by interactions with proteoglycans (PGs) substituted sulfated glycosaminoglycans (GAGs). The major GAG cornea is keratan sulfate (KS), which N-linked to one of three PG core proteins. To ascertain the importance carbohydrate chain sulfation step KS functionality, we generated a strain mice targeted gene deletion Chst5, encodes an N-acetylglucosamine-6-O-sulfotransferase that integral chains. Corneas homozygous mutants were...
Glaucoma is characterized by chronic progressive optic nerve damage and retinal ganglion cell death. Although extensive research has been conducted on neuroprotection for cells, there still no treatment clinical use. Recent evidence shows that extracellular vesicles isolated from a variety of stem cells are efficacious in neuroprotection. In this study, we tested the novel vesicle source progenitor R-28 line vitro vivo. We their therapeutic efficacy terms survival an vivo glaucoma model,...
Purpose: The presence of fibrillin-rich elastic fibers in the cornea has been overlooked recent years. aim current study was to elucidate their functional role using a mouse model for Marfan syndrome, defective fibrillin-1, major structural component microfibril bundles that constitute most fibers. Methods: Mouse corneas were obtained from animals with heterozygous fibrillin-1 mutation (Fbn1+/−) and compared wild type controls. Corneal thickness radius curvature calculated optical coherence...
Microbial keratitis is a severe, sight-threatening condition caused by various pathogens. Eyedrops are the standard delivery modality for treating these disorders; however, blinking reflex, elevated tear production, and nasolacrimal drainage eliminate much of instilled dose within few seconds. Therefore, eyedrops must be applied repeatedly prolonged periods. The present study aimed to probe more effective ocular chlorhexidine based upon drug-loaded hydrogel contact lenses β-cyclodextrin...
Abstract The long-term survival of biomaterial implants is often hampered by surgery-induced inflammation that can lead to graft failure. Considering most corneas receiving grafts are either pathological or inflamed before implantation, the risk rejection heightened. Here, we show bioengineered, fully synthetic, and robust corneal be manufactured from a collagen analog (collagen-like peptide-polyethylene glycol hybrid, CLP-PEG) inflammation-suppressing polymeric 2-methacryloyloxyethyl...
The cornea relies on its organised extracellular matrix for maintaining transparency and biomechanical strength. Studies have identified an elastic fibre system within the human posterior cornea, thought to allow slight deformations in response internal pressure fluctuations eye. However, type of fibres that exist their roles remain elusive. aim this study was compare distribution organisation peripheral mouse elucidate how these integrate with trabecular meshwork, whilst characterising main...
Elastic fibres provide tissues with elasticity and flexibility. In the healthy human cornea, elastic are limited to posterior region of peripheral stroma, but their specific functional role remains elusive. Here, we examine physical structural characteristics cornea during development in mgΔloxPneo dominant-negative mouse model for Marfan syndrome, which physiological extracellular matrix its elastic-fibre rich is disrupted by presence a dysfunctional fibrillin-1 glycoprotein. Optical...
Purpose: To investigate the initial events in development of human cornea, focusing on cell migration, and extracellular matrix synthesis organization. determine whether elastic fibers are present during early corneal development. Methods: Human corneas were collected from week 7 to 17 An fiber-enhancing stain, tannic acid–uranyl acetate, was applied all tissue. Three-dimensional serial block-face scanning electron microscopy combined with conventional transmission used analyze stroma....
As the outer lens in eye, cornea needs to be strong and transparent. These properties are governed by arrangement of constituent collagen fibrils, but mechanisms how this develops mammals is unknown. Using novel 3-dimensional scanning conventional transmission electron microscopy, we investigated developing mouse cornea, focusing on invading cells, extracellular matrix types deposited at different stages. Unlike well-studied chick, had no acellular primary stroma. Collagen fibrils initially...
Many myodocopid ostracods are unusual in that they have well-developed compound eyes yet must view their environment through a shell. The cypridinid Macrocypridina castanea is relatively large among (about 5-10 mm) and pelagic predator. This species possess highly pigmented shells with transparent region lying just above the eye. Here we examine ultrastructure transparency of this window using electron microscopy, serial-block face scanning microscopy X-ray diffraction analysis optical...
Fibrillin-1 is a pivotal structural component of the kidney’s glomerulus and peritubular tissue. Mutations in fibrillin-1 gene result Marfan syndrome (MFS), an autosomal dominant disease connective Although kidney not considered classically affected organ MFS, several case reports describe glomerular patients. Therefore, this study aimed to characterize mgΔ lpn -mouse model MFS. Affected animals presented significant reduction glomerulus, glomerulus-capillary, urinary space, fibronectin...
Abstract Primary crustacean cell culture was introduced in the 1960s, but to date limited lines have been established. Skogsbergia lerneri is a myodocopid ostracod, which has body enclosed within thin, durable, transparent bivalved carapace, through eye can see. The epidermal layer inner surface of carapace and responsible for synthesis. purpose present study develop an vitro tissue method S. . First, optimal environment viability this ascertained, while maintaining its proliferative...
Elastic fibres constitute an important component of the extracellular matrix and currently are subject intensive study in order to elucidate their assembly, function involvement cell-matrix interactions disease. However, few studies date have investigated 3D architecture elastic fibre system bulk tissue. We describe a protocol for preparation tissue samples, including primary fixation backscatter electron contrast-enhancement steps, through dehydration into stable resin-embedded blocks...
Fuchs' endothelial corneal dystrophy (FECD) is a common sight-threatening condition characterised by pathological changes in the posterior cornea. Here we report observations light, transmission and volume scanning electron microscopy on endothelium matrix associated with characteristic deformations of Descemet's membrane, termed guttae. Specimens were archived full-thickness human tissue, removed during graft surgery, that had been fixed, stained embedded conventional processing methods for...