A5051689665- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Intraocular Surgery and Lenses
- Glaucoma and retinal disorders
- Connexins and lens biology
- Ocular Infections and Treatments
- Proteoglycans and glycosaminoglycans research
- Periodontal Regeneration and Treatments
- Ophthalmology and Visual Impairment Studies
- Ocular Diseases and Behçet’s Syndrome
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Lymphatic System and Diseases
- Collagen: Extraction and Characterization
- Dermatological and Skeletal Disorders
- RNA Interference and Gene Delivery
- Skin Protection and Aging
- Angiogenesis and VEGF in Cancer
- Silk-based biomaterials and applications
- Dermatologic Treatments and Research
- Bone Tissue Engineering Materials
- Wound Healing and Treatments
- Aldose Reductase and Taurine
- Protease and Inhibitor Mechanisms
Linköping University Hospital
2006-2024
Linköping University
2014-2023
Massachusetts Eye and Ear Infirmary
1983-2017
Harvard University
1983-2017
Smith-Kettlewell Eye Research Institute
2017
Marie Curie
2010
European Union
2010
University of Ottawa
2006-2007
Smart Eye (Sweden)
2007
Karolinska Institutet
1993-2002
A biosynthetic cornea is stably integrated with host tissues for 2 years after implantation and produces nerve regeneration vision improvement.
We developed cell-free implants, comprising carbodiimide crosslinked recombinant human collagen (RHC), to enable corneal regeneration by endogenous cell recruitment, address the worldwide shortage of donor corneas. Patients were grafted with RHC implants. Over four years, regenerated neo-corneas stably integrated without rejection, long immunosuppression regime needed cornea patients. There was no recruitment inflammatory dendritic cells into implant area, whereas, even immunosuppression,...
To develop a simple corneal substitute from cross-linked collagen.Porcine type I collagen (10%; pH 5), was mixed with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The final homogenous solution molded to dimensions, cured, then implanted into rabbits minipigs by lamellar keratoplasty. implants were followed for up 6 months after surgery. Clinical examinations of the cornea included detailed slit lamp biomicroscopy, in vivo confocal microscopy, topography...
LiQD Cornea is a liquid in situ gelling, pro-regeneration hydrogel for treating corneal blindness and repairing perforations.
The severe worldwide shortage of donor organs, and pathologies placing patients at high risk for rejecting conventional cornea transplantation, have left many corneal blind untreated. Following successful pre-clinical evaluation in mini-pigs, we tested a biomaterials-enabled pro-regeneration strategy to restore integrity an open-label observational study six patients. Cell-free implants comprising recombinant human collagen phosphorylcholine were grafted by anterior lamellar keratoplasty...
Abstract. Purpose: To study the change in expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR‐2 rabbit cornea limbus following a penetrating, central corneal alkali burn. The influence different cells on VEGF was studied by excluding granulocytes from wound area. Methods: Fourteen New Zealand white rabbits were subjected to 5‐mm diameter, burn one eye under general anaesthesia. Seven given injections fucoidin for 36 hours. killed after hours corneas excised with...
Apoptosis was studied in rabbit corneas as a possible mechanism of cell death after photokeratitis induced by different UV wavelengths.Fourteen albino were exposed to 280- and 310-nm radiation (UVR) 10-nm full wavebands at doses that cause biomicroscopically significant keratitis (0.12 J/cm2 for 280 nm 0.47 310 nm). Animals killed 24 76 h exposure. Corneas processed light transmission electron microscopy situ end labeling fragmented DNA using modification the TUNEL technique.Corneas 280-nm...
To compare the efficacies of recombinant human collagens types I and III as corneal substitutes for implantation.Recombinant collagen (13.7%) type or was thoroughly mixed with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide N-hydroxysuccinimide. The final homogenous solution either molded into sheets in vitro studies implants appropriate dimensions transplantation minipigs. Animals were observed up to 12 months after surgery. Clinical examinations cornea included detailed slit lamp...
Loss of vision due to corneal disease or trauma affects over 10 million individuals worldwide. For many, trans-plantation is the only treatment, but supply good quality donor tissue cannot meet demand, especially in developing world. To address this need, several substitutes have been proposed;1, 2 however, date, clinically tested humans fully synthetic prostheses (keratoprostheses).3, 4 Although improving, complications with prostheses, including retroprosthetic membrane formation,...
To investigate morphologic alterations in the limbal palisades of Vogt a progressive form stem cell deficiency.Twenty Norwegian subjects (40 eyes) with congenital aniridia and 9 healthy family members (18 without were examined. Clinical grade aniridia-related keratopathy (ARK) was assessed by slit-lamp biomicroscopy, tear production quality, corneal thickness, sensitivity additionally measured. The superior inferior central cornea examined laser scanning vivo confocal microscopy (IVCM).In an...
Abstract Corneas with severe pathologies have a high risk of rejection when conventionally grafted human donor tissues. In this early observational study, we bioengineered corneal implants made from recombinant collagen and synthetic phosphorylcholine polymer into three patients for whom cornea transplantation carried transplant failure. These suffered ulcers recurrent erosions preoperatively. The provided relief pain discomfort, restored integrity by promoting endogenous regeneration...
PEG-conjugated collagen-like peptides promote corneal regeneration in a pig cornea.
Short collagen-like peptides (CLPs) are being proposed as alternatives to full-length collagen for use in tissue engineering, on their own soft hydrogels, or conjugated synthetic polymer mechanical strength. However, despite intended clinical use, little is known about safety and efficacy, mechanism of action degree similarity the counterparts they mimic. Here, we show functional equivalence a CLP polyethylene glycol (CLP-PEG) recombinant human vitro promoting stable regeneration corneal...
Biomaterials designed to replace the diseased cornea could be used treat corneal blindness where human donor tissue is in short supply, but challenges are integration of biomaterials with host and cells, avoiding a rapid material degradation maintaining transparency. Additionally, implantation surgery often triggers an aggressive wound healing response that can lead thinning opacity. Here, we report collagen-based hydrogel transparency mechanical properties suitable for replacing substantial...
AIM--To determine the relation between epithelial hyperplasia and regression of effect after photorefractive keratectomy (PRK). METHODS--Seventy unilaterally treated patients with PRK were examined. All eyes had been Summit excimer laser 27 (SD 7) months previously zone diameters 4.1 to 5.0 mm. The untreated fellow served as controls. Epithelial thickness was measured centrally a thin slit optical pachometer manifest subjective refraction performed. RESULTS--The epithelium 21% thicker in eye...