A5025674343- Advances in Oncology and Radiotherapy
- Zebrafish Biomedical Research Applications
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Economic and Financial Impacts of Cancer
- Radiation Therapy and Dosimetry
- S100 Proteins and Annexins
- Immune cells in cancer
- Corneal Surgery and Treatments
- Colorectal Cancer Treatments and Studies
- Retinal Diseases and Treatments
- Advanced Radiotherapy Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Histone Deacetylase Inhibitors Research
- Chemokine receptors and signaling
- Estrogen and related hormone effects
- Lymphatic System and Diseases
- Circadian rhythm and melatonin
- Corneal surgery and disorders
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Cancer Genomics and Diagnostics
- Retinal Development and Disorders
- Ocular Oncology and Treatments
- Cancer-related Molecular Pathways
Linköping University
2016-2025
Novo Nordisk (Denmark)
2007-2025
Ministry of Health of the Russian Federation
2018
Karolinska Institutet
2008-2017
Block Center
2014-2015
Institute of Molecular and Cell Biology
2009-2013
Svenska Örtmedicinska Institute
2013
Novel therapeutic targets of estrogen receptor (ER)-positive breast cancers are urgently needed because current antiestrogen therapy causes severe adverse effects, nearly 50% patients intrinsically resistant, and the majority recurrences have maintained ER expression. We investigated role estrogen-dependent chemokine expression subsequent cancer growth in human tissues experimental models.For vivo sampling chemokines, microdialysis was used women or normal tissue before after tamoxifen...
Mechanisms underlying pathological angiogenesis in relation to hypoxia tumor invasion and metastasis remain elusive. Here, we have developed a zebrafish model that allows us study the role of under normoxia arbitrating early events metastatic cascade at single cell level. Under normoxia, implantation murine T241 fibrosarcoma into perivitelline cavity developing embryos transgenic fli1:EGFP did not result significant dissemination, invasion, metastasis. In marked contrast, substantial cells...
Mechanistic understanding and defining novel therapeutic targets of diabetic retinopathy age-related macular degeneration (AMD) have been hampered by a lack appropriate adult animal models. Here we describe simple highly reproducible fli-EGFP transgenic zebrafish model to study retinal angiogenesis. The vasculature in the is organized hypoxia-induced neovascularization occurs predictable area capillary plexuses. New vessels vascular sprouts can be accurately measured quantified. Orally...
Although VEGF-B was discovered as a VEGF-A homolog long time ago, the angiogenic effect of remains poorly understood with limited and diverse findings from different groups. Notwithstanding, drugs that inhibit together other VEGF family members are being used to treat patients various neovascular diseases. It is therefore critical have better understanding underlying mechanisms. Using comprehensive in vitro vivo methods models, we reveal here for first an unexpected surprising function...
Abstract Purpose To examine the role of RhoB expression in relation to chemotherapy response, clinical outcomes and associated signaling pathways colorectal cancer patients. Materials methods The study included 5 colon cell lines, zebrafish embryos 260 patients treated with 5-fluorouracil (5-FU) oxaliplatin (OXL). consisted CRISPR/Cas9, reactive oxygen species (ROS), caspase-3 activity, autophagy flux, in-silico RNA sequencing immunohistochemistry. Gene analysis pathway were conducted using...
Molecular mechanisms underlying circadian-regulated physiological processes remain largely unknown. Here, we show that disruption of the circadian clock by both constant exposure to light and genetic manipulation key genes in zebrafish led impaired developmental angiogenesis. A bmal1-specific morpholino inhibited angiogenesis embryos without causing obvious nonvascular phenotypes. Conversely, a period2 accelerated angiogenic vessel growth, suggesting Bmal1 Period2 display opposing effects....
Embryonic development depends on complex and precisely orchestrated signaling pathways including specific reduction/oxidation cascades. Oxidoreductases of the thioredoxin family are key players conveying redox signals through reversible posttranslational modifications protein thiols. The importance this during embryogenesis has recently been exemplified for glutaredoxin 2, a vertebrate-specific glutathione-disulfide oxidoreductase with critical role embryonic brain development. Here, we...
// Jhon A. Ochoa-Alvarez 1 , Harini Krishnan John G. Pastorino Evan Nevel David Kephart Joseph J. Lee Edward P. Retzbach Yongquan Shen Mahnaz Fatahzadeh 2 Soly Baredes 3 Evelyne Kalyoussef Masaru Honma 4 Martin E. Adelson 5 Mika K. Kaneko 6 Yukinari Kato Mary Ann Young Lisa Deluca-Rapone Alan Shienbaum Kingsley Yin Lasse D. Jensen 7 and Gary S. Goldberg Departments of Molecular Biology, Cell Pathology, School Osteopathic Medicine, Rowan University, Stratford, NJ, USA Department Diagnostic...
Abstract Estradiol (E2) plays a key role in breast cancer progression. Most recurrences express the estrogen receptor (ER), but nearly 50% of patients are resistant to antiestrogen therapy. Novel therapeutic targets ER-positive cancers needed. Protumoral neutrophils expressing lymphocyte function-associated antigen 1 (LFA-1) integrin may mediate metastasis, and TGFβ1 is major chemoattractant for neutrophils. The E2 neutrophil–ER+ cell interactions unknown. We studied this vivo using murine...
Purpose: Cancer metastasis can occur at the early stage of tumor development when a primary is microscopic size. In particular, interaction malignant cells with other cell types including cancer-associated fibroblasts (CAF) in promoting remains largely unknown. Here, we investigated role CAFs facilitating initial events cancer tumors were sizes.Experimental Design: Multicolor-coded and coimplanted into transparent zebrafish body single-cell level was monitored living animals. Healthy...
Corneal neovascularization is a sight-threatening condition caused by angiogenesis in the normally avascular cornea. Neovascularization of cornea often associated with an inflammatory response, thus targeting VEGF-A alone yields only limited efficacy. The NF-κB signaling pathway plays important roles inflammation and angiogenesis. Here, we study consequences inhibition activation through selective blockade IKK complex IκB kinase β (IKK2) using compound IMD0354, focusing on effects...
Abstract Estrogen receptor α (ERα) is a key regulator of breast growth and cancer development. Here, we report how ERα impacts these processes by reprogramming metabolism in malignant cells. We employed an integrated approach, combining genome-wide mapping chromatin-bound with estrogen-induced transcript metabolic profiling, to demonstrate that reprograms upon estrogen stimulation, including changes aerobic glycolysis, nucleotide amino acid synthesis, choline (Cho) metabolism. Cho...
Accurate predictions of tumor dissemination risks and medical treatment outcomes are critical to personalize therapy. Patient-derived xenograft (PDX) models in mice have demonstrated high accuracy predicting therapeutic outcomes, but methods for invasiveness early stages vascular/lymphatic still lacking. Here we show that a zebrafish (ZTX) platform based on implantation PDX tissue fragments recapitulate both outcome invasiveness/dissemination patients, within an assay time only 3 days.Using...
Abstract The circadian clock in animals and humans plays crucial roles multiple physiological processes. Disruption of homeostasis causes detrimental effects. Here, it is demonstrated that the disruption rhythm by genetic deletion mouse brain muscle ARNT‐like 1 (Bmal1) gene, coding for key transcription factor, augments an exacerbated fibrotic phenotype various tumors. Accretion cancer‐associated fibroblasts (CAFs), especially alpha smooth actin positive myoCAFs, accelerates tumor growth...
Abstract Epithelial ovarian cancer (EOC) is the gynaecological malignancy with highest mortality. Although adjuvant treatment carboplatin and paclitaxel leads to an objective response in ~80% of these patients, a majority will relapse within two years. Better methods for assessing long-term outcomes are needed. To address this, we established safe efficacious doses using IGROV-1 zebrafish-CDX models. Then fluorescently-labelled cell suspensions from 83 tumour biopsies collected at...