A5003276179- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Radiopharmaceutical Chemistry and Applications
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Angiogenesis and VEGF in Cancer
- Protein Kinase Regulation and GTPase Signaling
- T-cell and B-cell Immunology
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Chronic Lymphocytic Leukemia Research
- Reproductive Biology and Fertility
- Nanofabrication and Lithography Techniques
- Cancer Immunotherapy and Biomarkers
- 14-3-3 protein interactions
- Ubiquitin and proteasome pathways
- Toxin Mechanisms and Immunotoxins
- Cancer, Hypoxia, and Metabolism
Hospital Universitario Puerta de Hierro Majadahonda
2015-2025
Hospital Universitari Sant Joan de Reus
2020
Center of Molecular Immunology (Cuba)
2012-2014
Universidad Autónoma de Madrid
1992-2013
Consejo Superior de Investigaciones Científicas
1993-2011
Centro de Investigaciones Biológicas Margarita Salas
2001-2011
Centro Nacional de Biotecnología
2011
Weatherford College
2010
Mayo Clinic in Arizona
2003-2005
Mayo Clinic
2003
Recent evidence demonstrates that the protein kinase C & ( P K ) isoform is required for activation of nuclear factor KB (NF-KB) and mitogenic signaling in Xenopus oocytes mammalian cells.The mechanism whereby regulates NF-KB most probably involves a putative IKB molecular mass -50 kDa, which phosphorylates inactivates IKB.Tumor necrosis (TNFa) interleukin-1, besides activating phospholipase C-mediated breakdown phosphatidylcholine, also generate ceramide, produced by stimulation...
Pericytes and mesenchymal stem cells (MSCs) are ontogenically related, in fact, no significant phenotypic differences could be observed by flow cytometry. Transcriptome analysis of human pericytes MSCs revealed that 43 genes were up-regulated more than 10-fold compared with MSCs. Identification Toll-like receptor 4 (TLR4) as one the most abundant RNA species respect to confirmation TLR4 expression on cell surface led us obtain a comprehensive overview program lipopolysaccharide...
The zeta isoform of protein kinase C (zeta PKC) has been shown to be involved in the maturation Xenopus oocytes and mitogenic signaling fibroblasts. PKC also regulates important transcription factor nuclear kappa B, most probably by phosphorylation inhibitory molecule I B. mechanisms that control activity are still poorly characterized. This is not activated diacylglycerol but potently stimulated vitro products phosphatidylinositol 3-kinase (PI 3-kinase), which suggests at least one critical...
Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of number genes. NF-kappa is heterodimer 50- and 65-kDa subunits sequestered cytoplasm complexed to inhibitory protein I B. Following stimulation cells, dissociates from B, allowing its translocation nucleus, where it carries out transactivation function. The precise mechanism controlling activation involvement members kinase C (PKC) family isotypes have previously been investigated. It was found that phorbol...
The costimulation of immune cells using first-generation anti-4-1BB monoclonal antibodies (mAbs) has demonstrated anti-tumor activity in human trials. Further clinical development, however, is restricted by significant off-tumor toxicities associated with FcγR interactions. Here, we have designed an Fc-free tumor-targeted 4-1BB-agonistic trimerbody, 1D8N/CEGa1, consisting three single-chain variable fragments and anti-EGFR single-domain positioned extended hexagonal conformation around the...
Multiple myeloma is the second most common hematological malignancy in adults and remains an incurable disease. B cell maturation antigen (BCMA)–directed immunotherapy, including T cells bearing chimeric receptors (CARs) systemically injected bispecific engagers (TCEs), has shown remarkable clinical activity, several products have received market approval. However, despite promising results, patients eventually become refractory relapse, highlighting need for alternative strategies....
AbstractRecent studies have documented the involvement of atypical protein kinase C (aPKC) isoforms in important cellular functions such as cell proliferation and survival. Exposure cells to a genotoxic stimulus that induces apoptosis, UV irradiation, leads profound inhibition PKC activity vivo. In this study, we addressed relationship between phenomenon different proteins involved apoptotic response. We show (i) aPKC precedes UV-induced apoptosis; (ii) apoptosis are independent p53; (iii)...
A number of studies have demonstrated the activation phospholipase C-mediated hydrolysis phosphatidylcholine (PC-PLC) both by growth factors and product ras oncogene, p21ras. Evidence has been presented indicating that stimulation this phospholipid degradative pathway is sufficient to activate mitogenesis in fibroblasts as well it necessary for induction maturation Xenopus laevis oocytes. However, mechanism whereby PC-PLC transduces mitogenic signals triggered or oncogenes remains be...
Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of large variety cellular genes. However, mechanism whereby this nuclear is activated remains to be determined. In report, we present evidence that oocytes from Xenopus laevis, (i) ras p21- and phospholipase C (PLC)-mediated phosphatidylcholine (PC) hydrolysis activates NF-kappa (ii) protein kinase zeta subspecies involved activation response insulin/ras p21/PC-PLC. Thus, microinjection either p21 or PC-PLC, exposure...
Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of number genes. NF-kappa is heterodimer 50- and 65-kDa subunits sequestered cytoplasm complexed to inhibitory protein I B. Following stimulation cells, dissociates from B, allowing its translocation nucleus, where it carries out transactivation function. The precise mechanism controlling activation involvement members kinase C (PKC) family isotypes have previously been investigated. It was found that phorbol...
The redirection of T cell activity using bispecific antibodies is one the most promising cancer immunotherapy approaches currently in development, but it limited by cytokine storm-related toxicities, as well pharmacokinetics and tumor-penetrating capabilities current antibody formats. Here, we have engineered ATTACK (Asymmetric Tandem Trimerbody for Activation Cancer Killing), a novel cell-recruiting which combines three EGFR-binding single-domain (VHH; clone EgA1) with single CD3-binding...
Retargeting of T lymphocytes toward cancer cells by bispecific antibodies has demonstrated its therapeutic potential, with one such antibody approved for the treatment acute lymphoblastic leukemia (blinatumomab) and several other in clinical trials. However, improvement their efficacy selectivity solid tumors is still required. Here, we describe a novel tandem T-cell recruiting trispecific colorectal (CRC). This construct, termed engager (TriTE), consists CD3-specific single-chain Fv (scFv)...