A5052375579- Congenital heart defects research
- Advanced Fluorescence Microscopy Techniques
- Angiogenesis and VEGF in Cancer
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- bioluminescence and chemiluminescence research
- Photoacoustic and Ultrasonic Imaging
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Cardiomyopathy and Myosin Studies
- Coronary Interventions and Diagnostics
- Genomics and Rare Diseases
- Adipose Tissue and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Apelin-related biomedical research
- RNA modifications and cancer
Baylor College of Medicine
2016-2025
Core Laboratories (United States)
2022
Cardiovascular Institute Hospital
2022
Baylor Genetics
2021
University of Connecticut
2013
Institute of Molecular Biology and Biophysics
2012
Dickinson College
2011
Rice University
2010
Vanderbilt University
1990
Intratumoral collagen cross-links heighten stromal stiffness and stimulate tumor cell invasion, but it is unclear how cross-linking regulated in epithelial tumors. To address this question, we used Kras(LA1) mice, which develop lung adenocarcinomas from somatic activation of a Kras(G12D) allele. The tumors mice were highly fibrotic contained cancer-associated fibroblasts (CAF) that produced generated gels. In xenograft by injection wild-type with adenocarcinoma cells alone or combination...
ABSTRACT To date, three closely-related TGFβ genes have been found in the mouse; TGFβ1, TGFβ2 and TGFβ3. Previous experiments indicated that TGFβ1 may play important roles during mouse embryo-genesis. The present study now reports distribution of transcripts TGFβ3 comparison to other two reveals overlapping but distinct patterns RNA expression. is expressed a diverse array tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, amnion, as well...
Sunitinib malate is a multitargeted receptor tyrosine kinase inhibitor used in the treatment of human malignancies. A substantial number sunitinib-treated patients develop cardiac dysfunction, but mechanism sunitinib-induced cardiotoxicity poorly understood. We show that mice treated with sunitinib and coronary microvascular dysfunction exhibit an impaired response to stress. The physiological changes caused by are accompanied depletion pericytes. Pericytes cell type dependent on intact...
The subependymal zone (SEZ) of the lateral ventricles is one areas adult brain where new neurons are continuously generated from neural stem cells (NSCs), via rapidly dividing precursors. This neurogenic niche a complex cellular and extracellular microenvironment, highly vascularized compared to non-neurogenic periventricular areas, within which NSCs precursors exhibit distinct behavior. Here, we investigate possible mechanisms by matrix molecules their receptors might regulate this...
Despite extensive work showing the importance of blood flow in angiogenesis and vessel remodeling, very little is known about how changes diameter are orchestrated at cellular level response to mechanical forces. To define necessary for we performed live confocal imaging cultured mouse embryos during remodeling. Our data revealed that increase occurs via two distinct processes dependent on normal flow: fusions directed endothelial cell migrations. Vessel resulted a rapid change were...
Classical measures of vessel morphology, including diameter and density, are employed to study microvasculature in endothelial membrane labeled mice. These measurements prove sufficient for some studies; however, they less well suited quantifying changes microcirculatory networks lacking hierarchical structure. We demonstrate that automated multifractal analysis lacunarity may be used with classical methods quantify microvascular morphology.
Attachment, spreading, and organization of endothelial cells into tubule networks are mediated by interactions between in the extracellular microenvironment. Laminins key matrix components regulators cell adhesion, migration, proliferation. In this study, laminin-derived peptides were conjugated to poly(ethylene glycol) (PEG) monoacrylate covalently incorporated degradable PEG diacrylate (PEGDA) hydrogels investigate influence these on cellular adhesion function organizing networks....
The identification of causal variants in sequencing studies remains a considerable challenge that can be partially addressed by new gene-specific knowledge. Here, we integrate measures how essential gene is to supporting life, as inferred from viability and phenotyping screens performed on knockout mice the International Mouse Phenotyping Consortium essentiality carried out human cell lines. We propose cross-species classification across Full Spectrum Intolerance Loss-of-function (FUSIL)...
Tissue clearing for whole organ cell profiling has revolutionized biology and imaging exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as well the use hazardous, organic solvents prevent widespread adoption these methods. Here, we report a simple rapid method, EZ Clear, that can clear adult mouse 48 hr just three steps. Samples stay at room temperature remain hydrated throughout process,...
Single/selective-plane illumination, or light-sheet, systems offer several advantages over other fluorescence microscopy methods for live, 3D microscopy. These are valuable studying embryonic development in animal systems, such as Drosophila, C. elegans and zebrafish. The geometry of the light path this form requires sample to be accessible from multiple sides fixed place so that it can rotated around a single axis. Popular mounting include hanging specimen pin embedding 1-2% agarose....
Recurrent microdeletions of 8p23.1 that include GATA4 and SOX7 confer a high risk both congenital diaphragmatic hernia (CDH) cardiac defects. Although GATA4-deficient mice have CDH defects, no humans with defects attributed to mutations been reported CDH. We were also unable identify deleterious sequence changes in cohort. This suggested haploinsufficiency another gene may contribute, along GATA4, the development To determine if SOX7—another transcription factor encoding gene—contributes...
The role of the Hippo signaling pathway in cranial neural crest (CNC) development is poorly understood. We used Wnt1Cre and Wnt1Cre2SOR drivers to conditionally ablate both Yap Taz CNC mice. When using either Cre driver, deficiency resulted enlarged, hemorrhaging branchial arch blood vessels hydrocephalus. However, embryos had an open tube phenotype that was not evident embryos. In O9-1 cells, loss impaired smooth muscle cell differentiation. RNA-sequencing data indicated regulate genes...
Identifying genes that are important for embryo development is a crucial first step towards understanding their many functions in driving the ordered growth, differentiation and organogenesis of embryos. It can also shed light on origins developmental disease congenital abnormalities. Current international efforts to examine gene function mouse provide unique opportunity pinpoint involved embryogenesis, owing emergence embryonic lethal knockout mutants. Through internationally coordinated...
The International Mouse Phenotyping Consortium is generating null allele mice for every protein-coding gene in the genome and characterizing these to identify gene–phenotype associations. While CRISPR/Cas9-mediated production highly efficient, generation of conditional alleles has proven be more difficult. To test feasibility using CRISPR/Cas9 editing generate knockout this large-scale resource, we employed Cas9-initiated homology-driven repair (HDR) with short long single stranded...
Highlights•Apela (a.k.a. Elabela, Ende, and Toddler) peptide is a critical ligand for APLNR in mouse development•Embryonic lethal cardiovascular defects Apela mutants may rely on circulation•Penetrance of affected phenotypes not rescued by Apelin•Apela mutation impacts early endothelial, erythroid, myeloid mesoderm derivativesSummaryApela (also known as small signaling that activates the G-protein-coupled receptor Aplnr to stimulate cell migration during zebrafish gastrulation. Here, using...
Optical coherence tomography (OCT) holds great promise as a routine research tool for 3-D analysis of mammalian embryos. However, despite the depth penetration afforded by this imaging modality, light attenuation in tissues imposes limitations. Here we studied optical clearing effect different concentrations glycerol mouse Depth- and time-resolved profiles OCT signal enhancement are presented. We found that application 50% resulted 51.5±12.5% improvement signal, while 25% enhanced 25.2±7.3%...
The most common and lethal birth defects affect the cardiovascular (CV) system. mouse is a superior model for identifying understanding mammalian CV defects, but there great need tools that can detect early subtle deficiencies in cardiac function embryos. We combined swept source optical coherence tomography (SS-OCT) with live embryo culture protocols to generate structural two-dimensional three-dimensional imaging hemodynamic measurements 8.5 day just few hours after beginning of heartbeat....
Abstract Sox9‐EGFP knockin mice were generated to label Sertoli cells and visualize testis cord formation during development. Confocal microscopy morphometric analysis of developing cords performed. Serial histological sections used for three‐dimensional reconstruction. Initially, gonad length decreased from embryonic day (E) 11.5 E13.5, but increased thereafter, while width doubled every 12 hours E11.5 through E14.5. At E12.5, the average number was 12.5, whereas this 10.4 at E13.5 Cord a...
Abstract The development of the cardiovascular system is a highly dynamic process dependent on multiple signaling pathways regulating proliferation, differentiation, migration, cell–cell and cell‐matrix interactions. To characterize cell tissue dynamics during formation in mice, we generated novel transgenic mouse line, Tg(Flk1::myr‐mCherry), which endothelial membranes are brightly labeled with mCherry, red fluorescent protein. Tg(Flk1::myr‐mCherry) mice viable, fertile, do not exhibit any...
Live imaging of normal and abnormal vascular development in mammalian embryos is important tool embryonic research, which can potentially contribute to understanding, prevention treatment cardiovascular birth defects. Here, we used speckle variance analysis swept source optical coherence tomography (OCT) data sets acquired from live mouse reconstruct the 3-D structure vasculature. Both Doppler OCT algorithms were structure. The results demonstrates that provides more accurate representation...
Although the mouse is a superior model to study mammalian embryonic development, high-resolution live dynamic visualization of embryos remain technical challenge. We present optical coherence tomography as novel methodology for imaging through uterine wall thereby allowing time lapse analysis developmental processes and direct phenotypic developing embryos. assessed capability proposed visualize structures living embryo from stages 12.5 18.5 days postcoitus. Repetitive in utero demonstrated....