A5008847830- Microbial Inactivation Methods
- Connexins and lens biology
- Advanced biosensing and bioanalysis techniques
- Hearing, Cochlea, Tinnitus, Genetics
- Magnetic and Electromagnetic Effects
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Heme Oxygenase-1 and Carbon Monoxide
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Toxin Mechanisms and Immunotoxins
- Ion channel regulation and function
- Nanoparticle-Based Drug Delivery
- Nicotinic Acetylcholine Receptors Study
- Plant Genetic and Mutation Studies
- Effects of Radiation Exposure
- Ion Channels and Receptors
- Viral Infectious Diseases and Gene Expression in Insects
- Biosensors and Analytical Detection
Université de Montpellier
2024
Institut de Génétique Humaine
2024
Centre National de la Recherche Scientifique
2024
Old Dominion University
2021-2023
Institute of Cell Biology and Neurobiology
2017-2021
Sapienza University of Rome
2016-2021
Roma Tre University
2017-2020
Università degli Studi Internazionali di Roma
2020
Recent progress in nanotechnology and its application to biomedical settings have generated great advantages dealing with early cancer diagnosis. The identification of the specific properties cells, such as expression particular plasma membrane molecular receptors, has become crucial revealing presence assessing stage development disease. Here we report a single cell screening approach based on Surface Enhanced Raman Scattering (SERS) microimaging. We fabricated SERS-labelled nanovector...
Background: Mutations leading to changes in properties, regulation or expression of connexin–made channels have been implicated twenty-eight distinct human hereditary diseases. Eight these result from variants connexin 26 (Cx26), a protein critically involved cell-cell signaling the inner ear and skin. Lack non-toxic drugs with defined mechanisms action poses serious obstacle therapeutic interventions for diseases caused by mutant connexins. In particular, molecules that specifically...
Numerous currently incurable human diseases have been causally linked to mutations in connexin (Cx) genes. In several instances, pathological generate abnormally active Cx hemichannels, referred also as "leaky" hemichannels. The goal of this study was assay the vivo efficacy a potent antagonist antibody targeting
Live imaging of translation based on tag recognition by a single-chain antibody is powerful technique to assess regulation in living cells. However, this approach challenging and requires optimization terms expression level detection sensitivity the system, especially multicellular organism. Here, we improved existing fluorescent tools developed new ones image quantify nascent Drosophila embryo mammalian We tested characterized five different green protein variants fused fragment variable...
Using microfluidics, ATP biosensors, multiphoton microscopy and genetically targeted mice, we show that release through connexin hemichannels, not pannexin 1 channels, underlies spontaneous Ca<sup>2+</sup> wave propagation in the greater epithelial ridge of developing cochlea.
Panx1 forms plasma membrane channels in brain and several other organs, including the inner ear. Biophysical properties, activation mechanisms modulators of have been characterized detail, however impact on auditory function is unclear due to conflicts published results. To address this issue, hearing performance cochlear Panx1−/− mouse strain, first with a reported global ablation Panx1, were scrutinized. Male female homozygous (Panx1−/−), hemizygous (Panx1+/−) their wild type (WT) siblings...
Antifolate plasmonic nanovectors (NVs) are proposed. SERS and cytotoxicity studies on non-cancer cancer cells reveal the efficient targeting killing of cells. The selectivity is optimized by coloading folate antifolate NV.
DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates the prevalent oxidized nucleosites, which can be misincorporated during replication, leading mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with ab initio calculations, for identification, detection, quantification of low concentrations nucleotides. We also show that Raman...
In this study, we used B16-F10 cells grown in the dorsal skinfold chamber (DSC) preparation that allowed us to gain optical access processes triggered by photodynamic therapy (PDT). Partial irradiation of a photosensitized melanoma cell death non-irradiated tumor cells. Multiphoton intravital microscopy with genetically encoded fluorescence indicators revealed bystander was mediated paracrine signaling due adenosine triphosphate (ATP) release from connexin (Cx) hemichannels (HCs)....
Abstract Although electric field–induced cell membrane permeabilization (electroporation) is used in a wide range of clinical applications from cancer therapy to cardiac ablation, the cellular- and molecular-level details processes that determine success or failure these treatments are poorly understood. Nanosecond pulsed field (nsPEF)–based tumor therapies known have an immune component, but whether how cells sense electroporative damage respond it not been demonstrated. Damage-...
In cells, photosensitizer (PS) activation by visible light irradiation triggers reactive oxygen species (ROS) formation, followed a cascade of cellular responses involving calcium (Ca2+) and other second messengers, resulting in cell demise. Cytotoxic effects spread to nearby cells not exposed poorly characterized so-called “bystander effects”. To elucidate the mechanisms involved bystander death, we used both genetically encoded biosensors fluorescent dyes. particular, monitored kinetics...
Abstract Intense pulsed electric fields (PEF) are increasingly employed for tumor ablation, including inoperable tumors, bleeding metastases, and tumors unresponsive to alternative treatments. PEF disrupt the membrane barrier of cells leading irreversible changes in homeostasis cell death. Using different models, we found that treatment with nanosecond duration (nsPEF) enhance host antitumor immune response. The strongest effect was measured mice bearing CT26 colon carcinoma where local...