A5024876310- Biochemical and Molecular Research
- Membrane-based Ion Separation Techniques
- Enzyme Structure and Function
- Ion channel regulation and function
- Anesthesia and Pain Management
- Bacterial Genetics and Biotechnology
- Plant Stress Responses and Tolerance
- Electrochemical Analysis and Applications
- Cancer-related molecular mechanisms research
- Ion Transport and Channel Regulation
- Acupuncture Treatment Research Studies
- Nausea and vomiting management
- Microbial Metabolic Engineering and Bioproduction
- Ferroptosis and cancer prognosis
- Neurological Disease Mechanisms and Treatments
- Protein Structure and Dynamics
- Electrochemical sensors and biosensors
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Hemoglobin structure and function
- Fungal and yeast genetics research
- Medical Imaging and Analysis
- Connexins and lens biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Tuberculosis Research and Epidemiology
Hunan University of Traditional Chinese Medicine
2020-2024
ShanghaiTech University
2015-2023
University of Chinese Academy of Sciences
2023
Center for Excellence in Molecular Cell Science
2018-2023
Chinese Academy of Sciences
2002-2023
Fujian Medical University
2021
The University of Texas Southwestern Medical Center
2012-2016
Fujian University of Traditional Chinese Medicine
2012-2014
Beijing Jishuitan Hospital
2010-2011
Peking University
2010-2011
Maintaining Equilibrium Na + /Ca 2+ exchangers (NCX) are membrane transporters that maintain the homeostasis of cytosolic Ca and play an essential role in signaling. Despite a long history physiological work large body functional data, structural basis underlying ion exchange mechanism NCX is poorly understood. Liao et al. (p. 686 ; see Perspective by Abramson ) present high-resolution crystal structure from Methanococcus jannaschii demonstrate this archaeal catalyzes -exchange reactions...
ABSTRACT Bacteria engage in chemical signaling, termed quorum sensing (QS), to mediate intercellular communication, mimicking multicellular organisms. The LuxR family of QS transcription factors regulates gene expression, coordinating population behavior by endogenous acyl homoserine lactones (AHLs). However, some bacteria (such as Escherichia coli ) do not produce AHLs. These orphans sense exogenous AHLs but also regulate the absence Importantly, this AHL-independent regulatory mechanism is...
A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR POLLUX, are required for the establishment legume-microbe symbioses by generating perinuclear Ca2+ spiking. Here we show that from Lotus japonicus is a highly selective channel whose activation requires cytosolic/nucleosolic Ca2+, contrary to previous suggestion it being K+ channel. Structurally, ligand-binding soluble region contains two tandem RCK (Regulator Conductance K+) domains,...
Volume-regulated anion channels (VRACs) are hexamers of LRRC8 proteins that crucial for cell volume regulation. N termini (NTs) the obligatory LRRC8A subunit modulate VRACs activation and ion selectivity, but underlying mechanisms remain poorly understood. Here, we report a 2.8-Å cryo-electron microscopy structure human displays well-resolved NTs. Amino-terminal halves NTs fold back into pore constrict permeation path, thereby determining selectivity together with an extracellular filter...
Background: Mutations leading to changes in properties, regulation or expression of connexin–made channels have been implicated twenty-eight distinct human hereditary diseases. Eight these result from variants connexin 26 (Cx26), a protein critically involved cell-cell signaling the inner ear and skin. Lack non-toxic drugs with defined mechanisms action poses serious obstacle therapeutic interventions for diseases caused by mutant connexins. In particular, molecules that specifically...
Background: Cerebral ischemia-reperfusion injury (CIRI) is a crucial factor leading to poor prognosis for ischemic stroke patients. As novel Chinese medicine formula, Naotaifang (NTF) was proven exhibit neuroprotective effect against stroke, clinically, and alleviate CIRI in animals. However, the mechanisms underlying beneficial have not been fully elucidated. Methods: In this study, we combined network pharmacology approach an vivo experiment explore specific effects of NTF treatment...
Abstract MLKL phosphorylation by RIP3 is the commitment step of necroptosis execution, which could induce activation featured as monomer-oligomer transition. Here, we reported that dimerization kinase-like domain was direct consequence triggered MLKL-phosphorylation. Two inter-dimer interfaces were found in crystal structure human MLKL. Mutations destroying both prevent RIP3-induced oligomerization and efficiently. Moreover, confirmed self-assembly internal coiled-coil region necessary for...
Dendrite morphogenesis is essential for a neuron to establish its receptive field and is, thus, the anatomical basis proper functioning of nervous system. The molecular mechanisms governing dendrite branching are not fully understood. Using multi-dendritic PVD in nematode Caenorhabditis elegans, we identify CATP-8/P5A ATPase as key regulator that controls translocation DMA-1 receptor endoplasmic reticulum (ER). specific signal peptide activity CATP-8 this process. Our results reveal P5A may...
The development of hepatic cancer is tightly regulated by multiple intracellular signaling pathways. Therefore, most currently-used anti-tumor agents, which typically target single pathway, might not always be therapeutically effective. Additionally, long-term use these agents probably generates drug resistance and unacceptable adverse effects. These problems increase the necessity for new chemotherapeutic approaches. Nitidine chloride (NC), a natural benzophenanthridine alkaloid, has been...
The Na + -Ca 2+ exchanger (NCX) is a ubiquitously expressed plasma membrane protein. It plays fundamental role in Ca homeostasis by moving out of the cell using electrochemical gradient as driving force. Recent structural studies homologous archaebacterial exchanger, NCX_Mj, revealed its outward configuration with two potential ion permeation pathways exposed to extracellular environment. Based on symmetry NCX_Mj structure, an atomic model inward-facing conformation was generated showing...
Sodium–calcium exchangers (NCXs) are membrane transporters that play an important role in Ca2+ homeostasis and signaling. The recent crystal structure of NCX_Mj, a member the NCX family from archaebacterium Methanococcus jannaschii, provided insight into atomistic details sodium–calcium exchange. Here, we extend these findings by providing detailed functional data on purified NCX_Mj using solid supported (SSM)–based electrophysiology, powerful but unexploited tool for studies electrogenic...
This study aims to investigate the effect of Naotaifang(NTF) on proteins associated with microglial polarization and glial scar in rat model cerebral ischemia reperfusion injury(CIRI). The CIRI was established by middle artery occlusion/reperfusion. 48 successfully modeled rats were randomized into 7 d, 14 NTF d groups(n=12). In addition, 12 SD selected as sham group. group administrated suspension at 27 g·kg~(-1)·d~(-1) gavage, sham, groups same volume normal saline every day gavage for...
Accurate partitioning of chromosomes during mitosis is essential for genetic stability and requires the assembly dynamic mitotic spindle proper kinetochore–microtubule attachment. The checkpoint (SAC) monitors incompleteness errors in attachment delays anaphase. SAC kinase Mps1 regulates recruitment downstream effectors to unattached kinetochores. also actively promotes chromosome alignment metaphase, but underlying mechanism not completely understood. Here, we show that through MCRS1, a...
The purpose of this study was to investigate whether treatment with electroacupuncture (EA) inhibited mitochondria-dependent apoptosis in annulus fibrosis (AF) cells a rat model cervical intervertebral disc degradation induced by unbalanced dynamic and static forces. Forty Sprague-Dawley rats were used study, which 30 underwent surgery induce degradation, 10 received EA at acupoints Dazhui (DU 14) Shousanli (LI 10). TUNEL staining measured assess AF cells, immunohistochemistry examine Bcl-2...