A5100409671- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Insect Resistance and Genetics
- RNA regulation and disease
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Enzyme Catalysis and Immobilization
- Mosquito-borne diseases and control
- Viral Infectious Diseases and Gene Expression in Insects
- Facial Rejuvenation and Surgery Techniques
- interferon and immune responses
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Asthma and respiratory diseases
- Electronic and Structural Properties of Oxides
- Dermatologic Treatments and Research
- Plant Virus Research Studies
- Pluripotent Stem Cells Research
- SARS-CoV-2 and COVID-19 Research
ShanghaiTech University
2016-2025
Affiliated Hospital of Hebei University
2024
Yunnan Agricultural University
2023-2024
Shanghai Clinical Research Center
2021-2024
Guangzhou Experimental Station
2022-2024
Affiliated Hospital of Qingdao University
2024
Qingdao University
2024
Jiangnan University
2021-2024
Central South University
2018-2024
Ruijin Hospital
2024
Transcription activator-like (TAL) effector nucleases (TALENs) have enabled the introduction of targeted genetic alterations into a broad range cell lines and organisms. These customizable are comprised programmable sequence-specific DNA-binding modules derived from TAL proteins fused to non-specific FokI cleavage domain. Delivery these cells has proven challenging as large size highly repetitive nature domain precludes their incorporation many types viral vectors. Furthermore, non-viral...
Cas12a-based systems, which detect specific nucleic acids via collateral cleavage of reporter DNA, display huge potentials for rapid diagnosis infectious diseases. Here, the Manganese-enhanced Cas12a (MeCas12a) system is described, where manganese used to increase detection sensitivity up 13-fold, enabling target RNAs as low five copies. MeCas12a also highly specific, and able distinguish between single nucleotide polymorphisms (SNPs) differing by a nucleotide. can severe acute respiratory...
Alpha interferon (IFN-α) induces the transfer of resistance to hepatitis B virus (HBV) from liver nonparenchymal cells (LNPCs) hepatocytes via exosomes. However, little is known about entry machinery and pathway involved in transmission IFN-α-induced antiviral activity. In this study, we found that macrophage exosomes uniquely depend on T cell immunoglobulin mucin receptor 1 (TIM-1), a A (HAV) receptor, enter for delivering anti-HBV Moreover, two primary endocytic routes infection,...
Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) contains cytokines and growth factors that can facilitate the regeneration repair of various tissues organs. In present study, protective activity ADSC-CM treatment was investigated in UVB-irradiated human keratinocyte cell line HaCaTs normal dermal fibroblasts (NHDFs). It found modulate expression signaling molecules early UVB responsive pathways, including mitogen activated protein kinases (MAPKs), activator 1...
Summary CRISPR/Cas‐base editing is an emerging technology that could convert a nucleotide to another type at the target site. In this study, A3A‐PBE system consisting of human A3A cytidine deaminase fused with Cas9 nickase and uracil glycosylase inhibitor was established developed in allotetraploid Brassica napus . We designed three sgRNAs ALS , RGA IAA7 genes, respectively. Base‐editing efficiency demonstrated be more than 20% for all genes. Target sequencing results revealed window ranged...
Designing protein mutants with both high stability and activity is a critical yet challenging task in engineering. Here, we introduce PRIME, deep learning model, which can suggest improved without any prior experimental mutagenesis data for the specified protein. Leveraging temperature-aware language modeling, PRIME demonstrated superior predictive ability compared to current state-of-the-art models on public dataset across 283 assays. Furthermore, validated PRIME’s predictions five...
The development of new methods for delivering proteins into cells is a central challenge advancing both basic research and therapeutic applications. We previously reported that zinc-finger nuclease are intrinsically cell-permeable due to the cell-penetrating activity Cys2–His2 domain. Here, we demonstrate genetically fused motifs can transport enzymes wide range primary transformed mammalian cell types. show domains mediate protein uptake at efficiencies exceed conventional transduction...
Although it is possible to generate neural stem cells (NSC) from somatic by reprogramming technologies with transcription factors, clinical utilization of patient‐specific NSC for the treatment human diseases remains elusive. The risk hurdles are associated viral transduction vectors induced mutagenesis, tumor formation undifferentiated cells, and factors‐induced genomic instability. Here we describe a vector‐free more efficient method induce mouse fibroblasts into using small molecules....
Zika virus (ZIKV) infection can cause fetal developmental abnormalities and Guillain-Barré syndrome in adults. Although progress has been made understanding the link between ZIKV microcephaly, pathology of ZIKV, particularly viral reservoirs human, remains poorly understood. Several studies have shown that compared to serum samples, patients' urine samples often a longer duration persistency higher load. This finding suggests an independent reservoir may exist human urinary system. Despite...
The emergence of CRISPR-Cas9 technology has revolutionized both basic and translational biomedical research. For Cas9 nuclease to exert genome editing activity, nuclear localization signal (NLS) derived from simian virus 40 (SV40) T antigen is commonly installed as genetic fusion direct the intracellular proteins nucleus cells. Notably, previous studies have shown that multiple SV40 NLS can improve targeting activity Cas9-derived genome-editing base-editing tools. In addition, multi-NLS...
Despite the tremendous success of combination antiretroviral therapy (ART) to treat human immunodeficiency virus (HIV) infection, durability and persistence latent reservoirs HIV-infected cells in patients remain obstacles achieving HIV cure. While technically challenging, most direct means eradicate is destroy provirus, thus ensuring that virions are not produced while preserving resident cells. Transcription activator-like effector nucleases (TALEN)─a genome editing method with high DNA...