A5100626384- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Hepatitis Viruses Studies and Epidemiology
- Total Knee Arthroplasty Outcomes
- Veterinary medicine and infectious diseases
- SARS-CoV-2 and COVID-19 Research
- Knee injuries and reconstruction techniques
- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Stroke Rehabilitation and Recovery
- Gastric Cancer Management and Outcomes
- Viral Infections and Immunology Research
- Biosensors and Analytical Detection
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Osteoarthritis Treatment and Mechanisms
- Phytochemistry and Bioactive Compounds
- Hip and Femur Fractures
- Animal Disease Management and Epidemiology
- PI3K/AKT/mTOR signaling in cancer
Texas A&M University
2022-2025
Mitchell Institute
2024-2025
Chinese Academy of Agricultural Sciences
2021-2024
Ministry of Agriculture and Rural Affairs
2017-2024
Agricultural Genomics Institute at Shenzhen
2021-2024
Jining Medical University
2023-2024
Nanjing Medical University
2024
Peking University Third Hospital
2020-2024
Peking University
2020-2024
Affiliated Hospital of Jining Medical University
2024
Abstract African swine fever virus (ASFV), the aetiological agent of (ASF), causes lethal haemorrhagic in domestic pigs with high mortality and morbidity has devastating consequences on global industry. On-site rapid sensitive detection ASFV is key to timely implementation control. In this study, we developed a rapid, instrument-free method based CRISPR/Cas12a technology lateral flow (named CRISPR/Cas12a-LFD). The limit CRISPR/Cas12a-LFD 20 copies genomic DNA per reaction, process can be...
Cas12a-based systems, which detect specific nucleic acids via collateral cleavage of reporter DNA, display huge potentials for rapid diagnosis infectious diseases. Here, the Manganese-enhanced Cas12a (MeCas12a) system is described, where manganese used to increase detection sensitivity up 13-fold, enabling target RNAs as low five copies. MeCas12a also highly specific, and able distinguish between single nucleotide polymorphisms (SNPs) differing by a nucleotide. can severe acute respiratory...
Background: HOX transcript antisense RNA (HOTAIR) is a long non-coding (lncRNA) widely involved in the progression of numerous malignancies. Whereas, potential molecular mechanism HOTAIR cervical cancer still needed to be elaborated.Methods: The expression and miR-143-3p were detected tissues cells by qRT-PCR. MTT flow cytometry analysis performed measure cell proliferation apoptosis. Bioinformatics, Dual-Luciferase reporter RIP used analyze possible correlation between HOTAIR, BCL2. Bax...
We have investigated the role of human endogenous retroviruses in multiple sclerosis by analyzing DNA patients and controls 4 cohorts for associations between polymorphisms near viral restriction genes or retroviral loci with one more intact almost-intact genes. found that SNPs gene TRIM5 were inversely correlated disease. Conversely, around locus, HERV-Fc1, showed a highly significant association The latter was limited to narrow region contains no other known conclude HERV-Fc1 play etiology...
Knowledge of the host factors required for norovirus replication has been hindered by challenges associated with culturing human noroviruses. We have combined proteomic analysis viral translation and complexes a CRISPR screen, to identify infection. The core stress granule component G3BP1 was identified as factor essential efficient murine infection, demonstrating conserved function across Norovirus genus. Furthermore, we show that functions in novel paradigm VPg-dependent initiation,...
The CRISPR-Cas system was developed into a molecular diagnostic tool with high sensitivity, low cost, and specificity in recent years. Colorimetric assays based on nanozymes offer an attractive point-of-care testing method for their cost of use user-friendly operation. Here, MnO2 nanozyme-mediated CRISPR-Cas12a instituted to detect SARS-CoV-2. nanorods linked magnetic beads via single-stranded DNA (ssDNA) linker used as oxidase-like nanozyme inducing the color change...
Abstract Genetic testing is crucial for precision cancer medicine. However, detecting multiple same-site insertions or deletions (indels) challenging. Here, we introduce CoHIT (Cas12a-based One-for-all High-speed Isothermal Test), a one-pot CRISPR-based assay indel detection. Leveraging an engineered AsCas12a protein variant with high mismatch tolerance and broad PAM scope, can use single crRNA to detect NPM1 gene c.863_864 4-bp in acute myeloid leukemia (AML). After optimizing parameters,...
Abstract Rapid and accurate diagnostic tests are fundamental for improving patient outcomes combating infectious diseases. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas12a‐based detection system has emerged as a promising solution on‐site nucleic acid testing. Nonetheless, the effective design of CRISPR RNA (crRNA) remains challenging time‐consuming. In this study, we propose an enhanced crRNA with deep learning Cas12a‐mediated diagnostics, referred to...
Traditional CRISPR/Cas9-based gene knockouts are generated by introducing DNA double-strand breaks (DSBs), but this may cause excessive damage or cell death. CRISPR-based cytosine base editors (CBEs) and adenine (ABEs) can facilitate C-to-T A-to-G exchanges, respectively, without DSBs. CBEs have been employed in a knockout strategy: CRISPR-STOP i-STOP changes single nucleotides to induce in-frame stop codons. However, strategy is not applicable for some genes, the unwanted mutations CBE...
Abstract Background Accurate preoperative planning is an important step for accurate reconstruction in total hip arthroplasty (THA). Presently, completed using either a two-dimensional (2D) template or three-dimensional (3D) mimics software. With the development of artificial intelligence (AI) technology, AI HIP, software based on can quickly and automatically identify acetabular femur morphology, match optimal prosthesis size. However, accuracy feasibility its clinical application still...
Abstract Detection of pathogens with single‐nucleotide variations is indispensable for the disease tracing, but remains technically challenging. The D614G mutation in SARS‐CoV‐2 spike protein known to markedly enhance viral infectivity difficult detect. Here, we report an effective approach called “synthetic mismatch integrated crRNA guided Cas12a detection” (symRNA‐Cas12a) detect D614 and G614 variants effectively. Using this method, systemically screened a pool crRNAs that contain all...
Drug-resistant tuberculosis (TB) is a serious public health problem and threat to global TB prevention control. Streptomycin (STR) the earliest classical anti-TB drug, it drug that generated resistance treatment, which limits its use in treating impedes control efforts. The rapid, economical, highly sensitive detection of STR-resistant may help reduce disease transmission morbimortality. CRISPR/CRISPR-associated protein (Cas) new-generation pathogen method can detect single-nucleotide...
Meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. injuries are difficult to heal frequently progress into structural breakdown, which then leads osteoarthritis. Regeneration of heterogeneous tissue engineering meniscus (TEM) continues be scientific translational challenge. The morphology, architecture, mechanical strength, functional applications the cultivated TEMs have not been able meet clinical needs, may due...