A5053164555- Hearing Loss and Rehabilitation
- Biosensors and Analytical Detection
- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Tuberculosis Research and Epidemiology
- Advanced biosensing and bioanalysis techniques
- Advanced Memory and Neural Computing
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Biology Techniques and Applications
- Antibiotic Resistance in Bacteria
- Noise Effects and Management
- Listeria monocytogenes in Food Safety
- Mycobacterium research and diagnosis
- Barrier Structure and Function Studies
- Neuroscience and Music Perception
- Bone Metabolism and Diseases
- Alzheimer's disease research and treatments
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Advanced Biosensing Techniques and Applications
- S100 Proteins and Annexins
- Multisensory perception and integration
- Escherichia coli research studies
- Bacterial Infections and Vaccines
China Medical University
2011-2025
Shenzhen Third People’s Hospital
2022-2025
Southern University of Science and Technology
2022-2025
Chinese Center For Disease Control and Prevention
2020-2024
Center for Disease Control
2024
Beijing Tongren Hospital
2015-2024
Capital Medical University
2015-2024
Changchun Institute of Technology
2024
Beijing Institute of Neurosurgery
2018-2023
Hangzhou First People's Hospital
2023
Nineteen patients with high-risk resected stage III and IV melanoma were immunized three tumor antigen epitope peptides from gp100, MART-1, tyrosinase emulsified adjuvant Montanide ISA 51 received a fully human anti-cytotoxic T-lymphocyte antigen-4 (anti-CTLA-4) monoclonal antibody MDX-010. Each of cohorts escalating doses vaccine primarily to evaluate the toxicities maximum-tolerated dose (MTD) MDX-010 vaccine. pharmacokinetics immune responses secondary end points.Peptide immunizations...
The Comprehensive Resistance Prediction for Tuberculosis: an International Consortium (CRyPTIC) presents here a data compendium of 12,289 Mycobacterium tuberculosis global clinical isolates, all which have undergone whole-genome sequencing and had their minimum inhibitory concentrations to 13 antitubercular drugs measured in single assay. It is the largest matched phenotypic genotypic dataset M. date. Here, we provide summary detailing breadth collected, along with description how isolates...
During the course of diagnostic surgical pathology, pathologists have established a large collection formalin-fixed, paraffin-embedded tissues that form invaluable resources for translational studies cancer and variety other diseases. Accessibility macromolecules in fixed tissue specimens is critical issue as exemplified by heat-induced antigen retrieval (AR) immunohistochemical (IHC) staining. On basis observations heating may also enhance situ hybridization (ISH) similarity...
Abstract We have devised a novel amplification strategy based on isothermal strand-displacement polymerization reaction, which was termed multiple cross displacement (MCDA). The approach employed set of ten specially designed primers spanning distinct regions target sequence and preceded at constant temperature (61–65 °C). At the assay temperature, double-stranded DNAs were dynamic reaction environment primer-template hybrid, thus high concentration annealed to template strands without...
Drug-resistant tuberculosis (TB) is a serious public health problem and threat to global TB prevention control. Streptomycin (STR) the earliest classical anti-TB drug, it drug that generated resistance treatment, which limits its use in treating impedes control efforts. The rapid, economical, highly sensitive detection of STR-resistant may help reduce disease transmission morbimortality. CRISPR/CRISPR-associated protein (Cas) new-generation pathogen method can detect single-nucleotide...
Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but pathogenesis E. meningitis remains elusive. penetration blood-brain barrier (BBB) critical step for development meningitis. Here, we identify Caspr1, a single-pass transmembrane protein, as host receptor virulence factor IbeA to facilitate BBB penetration. Genetic ablation endothelial Caspr1 and blocking IbeA-Caspr1 interaction effectively prevent into brain during in rodents. interacts with...
The paper enhances deep-neural-network-based inference in sensing applications by introducing a lightweight attention mechanism called the global module for multi-sensor information fusion. This is capable of utilizing collected from higher layers neural network to selectively amplify influence informative features and suppress unrelated noise at fusion layer. We successfully integrate this into new end-to-end learning framework, GIobalFusion, where two modules are deployed spatial modality...
Aims: Quercetin, a flavonoid present in vegetables, has anti-inflammatory properties and potential inhibitory effects on bone resorption. Up to date, the effect of quercetin lipopolysaccharide (LPS)-induced osteoclastogenesis not yet been reported. In current study, we evaluated LPS-induced osteoclast apoptosis Methods: RAW264.7 cells were non-treated, treated with LPS alone, or both quercetin. After treatment, number osteoclasts, cell viability, resorption measured. The expressions...
Microvascular basement membrane (BM) plays a pivotal role in the interactions of astrocyte with endothelium to maintain blood-brain barrier (BBB) homeostasis; however, significance and precise regulation endothelial cell-derived BM component BBB remain incompletely understood. Here, we report that conditional knockout Atg7 cells (Atg7-ECKO) leads astrocyte-microvascular disassociation brain. Our results reveal astrocytic endfeet detachment from microvessels leakage Atg7-ECKO mice....
Abstract Objective Dysfunction of calcium/calmodulin (CaM)–dependent kinase II (CaMKII) has been involved in hyperexcitability‐related disorders including epilepsy. However, the relationship between CaMKII and neuronal excitability remains unclear. Methods Neuronal was detected vivo vitro by electroencephalography (EEG), patch clamp multi‐electrode array (MEA), respectively. Next, we assessed currents voltage‐gated sodium channels (VGSCs) clamp, mRNA protein expressions VGSCs were determined...
The increasing prevalence of multidrug-resistant tuberculosis (MDR-TB) highlights the urgent need for an efficient approach to identify Mycobacterium complex (MTBC) strains resistant rifampicin (RIF) and isoniazid (INH). In response, we developed a CRISPR-Cas14a MTB RIF/INH platform that can detect most common mutations associated with RIF INH resistance. To evaluate sensitivity specificity our platform, carried out comprehensive assessment using clinical isolates M. sputum samples from TB...
Biochemotherapy can achieve high response rates in advanced melanoma, but the factors that influence regression and survival remain unknown. The present exploratory study tested hypothesis cytokine gene polymorphisms predict clinical outcome stage IV melanoma patients treated with biochemotherapy.Ninety were biochemotherapy, including cisplatin, vinblastine, dacarbazine combined interleukin (IL)-2 IFN-alpha either or without tamoxifen. Cytokine for IFN-gamma (+874A-->T) IL-10 (-1082G-->A)...
Shigella and Salmonella are frequently isolated from various food samples can cause human gastroenteritis. Here, a novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (MERT-LAMP) were successfully established validated for simultaneous detection of strains in only single reaction. Two sets MERT-LAMP primers 2 kinds pathogens designed ipaH gene spp. invA spp., respectively. Under the constant condition at 63°C, positive results yielded as short...
ABSTRACT Neurological disorders are associated with an increase in the permeability of human brain microvascular endothelial cells (HBMEC). Our previous findings have indicated that EphA2 could HBMEC. Recent evidence has linked and vascular growth factor (VEGF) to abnormalities response. However, it is unclear whether involved VEGF‐induced changes Here, were determined by measuring transendothelial electrical resistance (TEER) flux FITC‐dextran. We found knockdown HBMEC abolished reduction...
We have devised a novel isothermal amplification technology, termed endonuclease restriction-mediated real-time multiple cross displacement (ET-MCDA), which facilitated multiplex, rapid, specific and sensitive detection of nucleic-acid sequences at constant temperature. The ET-MCDA integrated strategy, restriction cleavage fluorescence technique. In the system, functional primer E-CP1 or E-CP2 was constructed by adding short sequence 5' end CP1 CP2, respectively, new labeled with fluorophore...
The Chinese medicinal herb, Panax notoginseng, has long been used to treat bone fractures and notoginseng saponins (PNS) could promote formation. Here, we investigated whether PNS osteogenesis of marrow stromal cells (BMSCs) through modulating the MAPK signaling pathways, which are implicated in BMSC osteogenesis. We found that markedly increased mineralization BMSCs by alizarin red S assays stimulate alkaline phosphatase activity these cells. Additionally, significantly mRNA levels...
Alzheimer's disease (AD) is a progressive neurodegenerative dementia characterized by pathological senile plaques composed of amyloid-β (Aβ) in the cerebral cortex and hippocampus. Bone marrow-derived monocytes patients with AD migrate across blood-brain barrier into brain, but are defective at clearing Aβ neuritic plaques. However, underlying mechanisms remain unclear. Here, AD, we found that cathepsin D, major lysosomal aspartic protease, was underexpressed monocytes, resulting degradation...
The level of granulocyte-macrophage colony-stimulating factor (GM-CSF) increases in some disorders such as vascular dementia, Alzheimer’s disease, and multiple sclerosis. We previously reported that disease patients, a high GM-CSF the brain parenchyma downregulated expression ZO-1, blood–brain barrier tight junction protein, facilitated infiltration peripheral monocytes across barrier. However, molecular mechanism underlying regulation ZO-1 by is unclear. Herein, we found erythroblast...