Esther Robinson
A5038183263- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Actinomycetales infections and treatment
- Neonatal and Maternal Infections
- Pneumonia and Respiratory Infections
- Orthopedic Infections and Treatments
- Bacterial Infections and Vaccines
- SARS-CoV-2 and COVID-19 Research
- Image Processing Techniques and Applications
- Infectious Diseases and Mycology
- Osteomyelitis and Bone Disorders Research
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Genomics and Rare Diseases
- Infective Endocarditis Diagnosis and Management
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA and protein synthesis mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Clinical Research Studies
Public Health England
2018-2024
UK Health Security Agency
2022-2024
National Health Service
2023
University of California, Irvine
2022
Kovai Medical Center and Hospital
2022
Government of the United Kingdom
2019-2020
Heartlands Hospital
2007-2020
Public Health Laboratory
2007-2020
National Public Health Laboratory
2020
Heart of England NHS Foundation Trust
2013-2018
Foodborne outbreaks of Salmonella remain a pressing public health concern. We recently detected large outbreak enterica serovar Enteritidis phage type 14b affecting more than 30 patients in our hospital. This was linked to community, national and European-wide cases. Hospital with are at high risk, require rapid response. initially investigated this by whole-genome sequencing using novel protocol on the Illumina MiSeq; we then integrated these data from surveillance sequencing, thereby...
Molecular diagnostics are considered the most promising route to achieving rapid, universal drug susceptibility testing for
The Comprehensive Resistance Prediction for Tuberculosis: an International Consortium (CRyPTIC) presents here a data compendium of 12,289 Mycobacterium tuberculosis global clinical isolates, all which have undergone whole-genome sequencing and had their minimum inhibitory concentrations to 13 antitubercular drugs measured in single assay. It is the largest matched phenotypic genotypic dataset M. date. Here, we provide summary detailing breadth collected, along with description how isolates...
The World Health Organization has a goal of universal drug susceptibility testing for patients with tuberculosis. However, molecular diagnostics to date have focused largely on first-line drugs and predicting susceptibilities in binary manner (classifying strains as either susceptible or resistant). Here, we used multivariable linear mixed model alongside whole genome sequencing quantitative microtiter plate assay relate genomic mutations minimum inhibitory concentration (MIC) 15,211...
BackgroundMycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing is widely used in high-income countries to determine Mycobacterium tuberculosis relatedness. Whole-genome sequencing (WGS) known deliver greater specificity, but no quantitative prospective comparison has yet been undertaken.MethodsWe studied isolates from the English Midlands, sampled consecutively between 1 January 2012 and 31 December 2015. In addition routinely performed MIRU-VNTR...
Mycobacterium abscessus is emerging as an important pathogen in chronic lung diseases, with concern regarding patient-to-patient transmission. The recent introduction of routine whole-genome sequencing (WGS) a replacement for existing reference techniques England provides opportunity to characterize the genetic determinants resistance.
Abstract Background Electronic decision support systems could reduce the use of inappropriate or ineffective empirical antibiotics. We assessed accuracy an open-source machine-learning algorithm trained in predicting antibiotic resistance for three Gram-negative bacterial species isolated from patients’ blood and urine within 48 h hospital admission. Methods This retrospective, observational study used routine clinical information collected between January 2010 October 2016 Birmingham, UK....
Six lineages of Mycobacterium tuberculosis sensu stricto (which excludes M . africanum) are described. Single-country or small observational data suggest differences in clinical phenotype between lineages. We present strain lineage and from 12,246 patients 3 low-incidence 5 high-incidence countries. used multivariable logistic regression to explore the effect on site disease cavities chest radiography, given pulmonary TB; multinomial investigate types extra-pulmonary TB, lineage; accelerated...
Mycobacterium abscessus has emerged as a significant clinical concern following reports that it is readily transmissible in health-care settings between patients with cystic fibrosis. We linked routinely collected whole-genome sequencing and usage data the aim of investigating extent to which such transmission explains acquisition without fibrosis England.In this retrospective observational study, we analysed consecutive M from England (beginning February, 2015, Nov 14, 2019) identify...
Mycobacterium tuberculosis is a priority pathogen for global control. A growing number of countries have begun to incorporate routine whole genome sequencing (WGS) support key aspects (TB) public health and clinical practice, including monitoring laboratory practices, detection surveillance drug resistance, guidance targeted interventions such as expanded contact investigation active case finding. However, enhanced structures, workforce capacity systems are required effective translation...
Based on current evidence, routine screening for group B streptococcus colonisation in late pregnancy should not be introduced the UK, as potential harms of unnecessary treatment with antibiotics may outweigh benefits, argue <b>Farah Seedat and colleagues</b>
Over 10-years of whole-genome sequencing (WGS) Mycobacterium tuberculosis in Birmingham presents an opportunity to explore epidemiological trends and risk factors for transmission new detail. Between 1st January 2009 15th June 2019, we obtained the first WGS isolate from every patient resident a postcode district covered by Birmingham's centralised service. Data on patients' sex, country birth, social risk-factors, anatomical locus disease, strain lineage were collected. Poisson harmonic...
M. tuberculosis grows slowly and is challenging to work with experimentally compared many other bacteria. Although microtitre plates have the potential enable high-throughput phenotypic testing of tuberculosis, they can be difficult read interpret. Here we present a software package, Automated Mycobacterial Growth Detection Algorithm (AMyGDA), that measures how much growing in each well 96-well plate. The plate used here has serial dilutions 14 anti-tuberculosis drugs, thereby permitting...
Mycobacterium tuberculosis is the leading cause of death from bacterial infection. Improved rapid diagnosis and antimicrobial resistance determination, such as by whole-genome sequencing, are required. Our aim was to develop a simple, low-cost method preparing DNA for sequencing direct M. tuberculosis-positive clinical samples (without culture). Simultaneous sputum liquefaction, bacteria heat inactivation (99°C/30 min), enrichment mycobacteria were achieved using an equal volume...
The detection of laboratory cross-contamination and mixed tuberculosis infections is an important goal clinical mycobacteriology laboratories. objective this study was to develop a method detect mixtures different Mycobacterium lineages in laboratories performing mycobacterial next-generation sequencing (NGS). setting the Public Health England National Mycobacteriology Laboratory Birmingham, which performs Illumina on DNA extracted from positive growth indicator tubes. We analyzed 4,156...
Abstract Mycobacterium abscessus is emerging as an important pathogen in chronic lung diseases with concern regarding patient to transmission. The recent introduction of routine whole genome sequencing (WGS) a replacement for existing reference techniques England provides opportunity characterise the genetic determinants resistance. We conducted systematic review catalogue all known resistance determining mutations. This knowledge was used construct predictive algorithm based on mutations...
A significant part of horizontal gene transfer is facilitated by genomic islands. Haemophilus influenzae island ICEHin1056 an archetype a that accounts for pandemic spread antibiotics resistance. has modular structure and harbors modules involved in type IV secretion integration. Previous studies have shown encodes functional system; however, other not been characterized yet. Here we show the module on 5' extremity consists 15 genes are well conserved number related Furthermore disrupting...
Contact tracing requires reliable identification of closely related bacterial isolates. When we noticed the reporting artifactual variation between Mycobacterium tuberculosis isolates during routine next-generation sequencing spp., investigated its basis in 2,018 consecutive M. In process used, clinical samples were decontaminated and inoculated into broth cultures; from positive cultures DNA was extracted sequenced, reads mapped, consensus sequences determined. We sequence determination,...
Abstract Eight lineages of Mycobacterium tuberculosis sensu stricto are described. Single-country or small observational data suggest differences in clinical phenotype between lineages. We present strain lineage and from 12,246 patients 3 low-incidence 5 high-incidence countries. used multivariable logistic regression to explore the effect on site disease cavities chest radiography, given pulmonary TB; multinomial investigate types extra-pulmonary TB, lineage; accelerated failure time Cox...
Abstract Drug susceptibility testing of M. tuberculosis is rooted in a binary susceptible/resistant paradigm. Whilst there are considerable advantages measuring the minimum inhibitory concentrations (MICs) panel drugs for an isolate it n ecessary to measure epidemiological cutoff values (ECOFF/ECVs) permit comparison with qualitative data. Here we present ECOFF/ECVs 13 anti-TB compounds, including bedaquiline and delamanid, derived from 20,637 clinical isolates collected by 14 laboratories...