A5083196451- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Cancer therapeutics and mechanisms
- Diagnosis and treatment of tuberculosis
- Bacterial Genetics and Biotechnology
- Infectious Diseases and Mycology
- CRISPR and Genetic Engineering
- Cancer Research and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Infectious Diseases and Tuberculosis
- Pneumonia and Respiratory Infections
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolic Engineering and Bioproduction
- Chronic Myeloid Leukemia Treatments
- Microbial infections and disease research
- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Biosensors and Analytical Detection
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Animal Genetics and Reproduction
- interferon and immune responses
- Bacterial biofilms and quorum sensing
Engelhardt Institute of Molecular Biology
2015-2025
Genetic Technologies (Australia)
2025
Russian Academy of Sciences
2011-2015
Ajinomoto (United States)
2009
Ajinomoto (Russia)
2005-2008
Genetika
2005-2008
Molecular diagnostics are considered the most promising route to achieving rapid, universal drug susceptibility testing for
To study the isolates with acquired resistance to bedaquiline and linezolid that were obtained from patients enrolled in a clinical of novel therapy regimen for drug-resistant TB Moscow, Russia.Linezolid was detected using MGIT 960 critical concentration 1 mg/L. The MIC determined proportion method. identify genetic determinants resistance, sequencing mmpR ( Rv0678 ), atpE , atpC pepQ Rv1979c rrl rplC rplD loci performed.A total 85 27 reduced susceptibility (MIC ≥0.06 mg/L) tested. Most...
Ethionamide and prothionamide are now included in group C of the WHO recommended drugs for treatment tuberculosis resistant to rifampicin multidrug-resistant tuberculosis. The clinical relevance ethionamide has increased with wide spread tuberculosis.We retrospectively analyzed 349 isolates obtained between 2016 2020. susceptibility was tested using both BactecTM MGITTM 960 system SensititreTM MYCOTB plate.The MIC increases total resistance a row from susceptible XDR strains. A significant...
Bedaquiline is a cornerstone drug for treating drug-resistant tuberculosis. We analyzed 11 isolates from 9 patients who were treated with bedaquiline-based regimen and remained culture-positive long after treatment start. In 4 of 8 resistant isolates, we found substitutions in AtpE, which encodes subunit c the Mycobacterium tuberculosis ATP synthase rarely identified clinical isolates. Ile66Met Glu61Asp 2 cases each. Additional mutations mmpL5, mmpL4, atpB genes could affect susceptibility...
Aim. To study the susceptibility of clinical strains M. tuberculosis complex (MTB) to delamanid using automated Bactec MGIT system. Material and methods. We studied 79 isolates from 78 TB patients treated in 2017-2023. 39 MTB were susceptibile 40 had different drug resistance profiles anti-tubercular drugs. Minimum inhibitory concentrations (MIC) Middlebrook 7H9 liquid medium determined system, results assessed critical concentration 0.06 μg/ml recommended by WHO. Whole genome sequencing was...
The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under separate regulatory controls. construction plasmid-less marker-less has advantages for the further practical exploitation these bacteria in industry. Such are usually constructed by sequential chromosome modifications including deletions integration genetic material. For purposes...
The steady rise in the spread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant (XDR-TB) requires rapid reliable methods to identify resistant strains. current molecular detect MTB resistance second-line drugs either do not cover an extended spectrum mutations be identified or are easily implemented clinical laboratories. A technique for detection M. has been developed using hybridisation analysis on microarrays. method allows identification within gyrA gyrB genes...
Immobilization of molecular probes in 3D hydrogel elements provides some essential advantages compared with conventional flat surfaces. In this article, an integrated technology based on the use low-density microarrays comprised hemispherical gel elements, developed at Engelhardt Institute Molecular Biology (Moscow, Russia) for various applications will be reviewed. The structure can adapted immobilization virtually any biological molecules a natural hydrophilic environment. discrimination...
Nucleic acid amplification tests are widely used in TB diagnostics. Priority tasks their development consist of increasing the specificity and sensitivity detection resistance to a wide spectrum anti-TB drugs. We developed multiplexed assay allowing 116 drug resistance-determining mutations rpoB, katG, inhA, ahpC, gyrA, gyrB, rrs, eis embB genes Mycobacterium tuberculosis complex genome six SNPs identify main lineages circulating Russia. The is based on 17 fragments using universal primer...
In addition to the obligatory pathogenic species of Mycobacterium tuberculosis complex and leprae, genus also includes conditionally that in rare cases can lead development nontuberculous mycobacterial diseases. Because mycobacteriosis have similar clinical signs, accurate identification causative agent a microbiology laboratory is important for diagnostic verification appropriate treatment. This report describes low-density hydrogel-based microarray containing oligonucleotide probes based...
The pentose-phosphate pathway (PPP) is an important part of central metabolism in many organisms. A pgl(-) mutation that decreases the efficiency second stage PPP has been described and mapped at approx. 17.2 min Escherichia coli chromosome more than 30 years ago. Although it recently shown deletion ORF ybhE leads to earlier detected Pgl(-) phenotype for E. mutant strain, 6-phosphogluconolactonase from this organism not characterized. In present, independent investigation we show DeltaybhE...
Background The goal of this study was to compare the consistency three assays for determination drug resistance Mycobacterium tuberculosis (MTB) strains with various profiles isolated from Moscow region. Methods A total 144 MTB clinical isolates a strong bias toward were examined using Bactec MGIT 960, Sensititre MycoTB, and microarray-based molecular assay TB-TEST detect substitutions in rpoB, katG, inhA, ahpC, gyrA, gyrB, rrs, eis, embB genes that are associated rifampin, isoniazid,...
We investigated the rise of nontuberculous mycobacteria (NTM) infections in Bulgaria, focusing on species identification and distribution from 2018 to 2022. Utilizing advanced diagnostic tools, including Hain Mycobacterium CM/AS method, Myco-biochip assay, whole-genome sequencing, study identifies characterizes a diverse range clinical samples. While M. avium, gordonae, fortuitum, chelonae were dominating, number rare also found. They include such as marseillense celatum. Moreover,...
Background: Linezolid, bedaquiline, and newer fluoroquinolones are currently placed as priority Group A drugs for the treatment of drug-resistant tuberculosis. The number reported linezolid-resistant clinical strains is still low, correlation molecular determinants with phenotype not perfect. Methods: We determined linezolid MICs isolates from Moscow region identified mutations in rplC rrl genes. Results: All 16 had previously or loci, 13 them bore a RplC C154R substitution. Detection this...