A5102708013- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Acute Lymphoblastic Leukemia research
- Advanced Biosensing Techniques and Applications
- Molecular Biology Techniques and Applications
- BRCA gene mutations in cancer
- Acute Myeloid Leukemia Research
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Glioma Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Epigenetics and DNA Methylation
- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Cutaneous Melanoma Detection and Management
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Melanoma and MAPK Pathways
- bioluminescence and chemiluminescence research
- Chronic Myeloid Leukemia Treatments
- Biosensors and Analytical Detection
Engelhardt Institute of Molecular Biology
2016-2025
Wockhardt (United States)
2024
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology
2014-2022
Ministry of Health of the Russian Federation
2017-2022
Russian Academy of Sciences
1997-2022
PTC Therapeutics (United States)
2019
Institute of Molecular Genetics
2018
Russian Cancer Research Center NN Blokhin
2016-2017
Pirogov Russian National Research Medical University
2016
Moscow City Oncology Hospital №62
2014
Abstract Background Germline TP53 gene variants are intricately linked to Li-Fraumeni syndrome, a rare and aggressive hereditary cancer syndrome. This study investigated the frequency spectrum of pathogenic associated with syndrome in large cohort mainly breast patients from Russia. Methods The analyzed 3,455 genomic DNA samples using next-generation sequencing panels whole-genome sequencing. Clinically significant were identified validated Sanger clinical family history characteristics...
Background . Recent studies emphasize the heterogeneity of cryptogenic ischemic stroke (IS), highlighting importance identifying clinical and genetic risk factors. Objective This study explores associations between markers affecting spontaneous induced platelet aggregation (PA) parameters in patients with unspecified IS according to TOAST criteria, aiming uncover potential factors understand disease’s pathogenetic mechanisms. Materials methods The included 196 diagnosed stroke. We examined...
Patients with metastatic melanoma are difficult to treat and have a very poor prognosis because of high resistance therapy. Recent evidence indicates that tumors could overcome death through autophagy, survival mechanism, which cancer cells use under lack energy nutrient deprivation. Melanoma different sensitivity temozolomide (TMZ) treatment. In this study, we showed the combination autophagy inhibitors chloroquine or LY294002 TMZ induced enhanced cytotoxicity alkylating agents on human...
The switch from oxidative phosphorylation to glycolysis in proliferating cancer cells, even under aerobic conditions, has been shown first 1926 by Otto Warburg. Today this phenomenon is known as the “Warburg effect” and recognized a hallmark of cancer. metabolic shift associated with alterations signaling pathways involved energy metabolism, including glucose uptake fermentation, regulation mitochondrial functions. Hexokinases (HKs), which catalyze step glycolysis, have identified play role...
The contribution of different mechanisms to the regulation gene expression varies for tissues and tumors. Complementation predicted mRNA–miRNA gene–transcription factor (TF) relationships with results correlation analyses derived specific tumor types outlines interactions functional impact in current biomaterial. We developed CrossHub software, which enables two-way identification most possible TF–gene interactions: on basis ENCODE ChIP-Seq binding evidence or Jaspar prediction co-expression...
Aspirin resistance (AR) is a pressing problem in current ischemic stroke care. Although the role of genetic variations widely considered, data still remain controversial. Our aim was to investigate contribution features laboratory AR measured through platelet aggregation with arachidonic acid (AA) and adenosine diphosphate (ADP) patients. A total 461 patients were enrolled. Platelet via light transmission aggregometry. Eighteen single-nucleotide polymorphisms (SNPs) ITGB3, GPIBA, TBXA2R,...
Glioblastoma cell lines derived from different patients are widely used in tumor biology research and drug screening. A key feature of glioblastoma is the high level inter- intratumor heterogeneity that accounts for treatment resistance. Our aim was to investigate whether maintained models. Single-cell RNA sequencing cellular composition a sample six patient-derived lines. Three preserved mutational profile original tumor, whereas three others differed their precursors. Copy-number variation...
Immobilization of molecular probes in 3D hydrogel elements provides some essential advantages compared with conventional flat surfaces. In this article, an integrated technology based on the use low-density microarrays comprised hemispherical gel elements, developed at Engelhardt Institute Molecular Biology (Moscow, Russia) for various applications will be reviewed. The structure can adapted immobilization virtually any biological molecules a natural hydrophilic environment. discrimination...
A giant congenital melanocytic nevus (GCMN) is found in 0.1% of live-born infants. If present, the lesion has a chance about 6% to develop into malignant melanoma. Both children and adults can be affected by melanoma arising nevus. Up 95% GCMNs harbor NRAS mutations, mutations BRAF, MC1R, TP53, GNAQ genes have also been described. The individualization therapy required, but diagnostic prognostic criteria remain controversial.We report two cases: 1) during first month life complicated with...
Biological microarrays (biochips) are analytical tools that can be used to implement complex integrative genomic and proteomic approaches the solution of problems personalized medicine (e.g., patient examination in order reveal disease long before manifestation clinical symptoms, assess severity pathological or infectious processes, choose a rational treatment). The efficiency biochips is predicated on their ability perform multiple parallel specific reactions allow one study interactions...
// Marina Emelyanova 1 , Lilit Ghukasyan Ivan Abramov 1, 2 Oxana Ryabaya Evgenia Stepanova Anna Kudryavtseva 3 Asiya Sadritdinova Cholpon Dzhumakova Tatiana Belysheva Sergey Surzhikov Lyudmila Lyubchenko Alexander Zasedatelev and Nasedkina Engelhardt Institute of Molecular Biology, Russian Academy Sciences, Moscow, Federation Blokhin Cancer Research Center, Ministry Health the Federation, P. Hertsen Moscow Oncology Institute, Correspondence to: Emelyanova, email: emel_marina@mail.ru...
Oncolytic viruses, including live attenuated measles virus (MV) vaccine strains, have recently been shown as promising therapeutic agents against human malignancies. In this study, the oncolytic potential of strain Leningrad-16 (L-16) MV was evaluated in a panel metastatic melanoma cell lines. The L-16 to replicate within cells mediating direct killing tumor cells, although all lines varied regard their ability respond infection, revealed by different pattern Interferon Stimulated Gene...
Glioblastoma multiforme (GBM) accounts for almost half of all primary malignant brain tumors in adults and has a poor prognosis. Here we demonstrated the oncolytic potential L-16 vaccine strain measles virus (MV) against human GBM cells characterized genetic patterns that determine sensitivity to therapy. MV replicated cells, seven out eight cell lines underwent complete or partial oncolysis. RNA-Seq analysis identified about 1200 differentially expressed genes (FDR < 0.05) with at least...
The angiotensin-converting enzyme inhibitor enalapril is hydrolysed to an active metabolite, enalaprilat, in the liver via carboxylesterase 1 (CES1). Previous studies show that variant rs71647871 CES1 gene affects pharmacokinetics of on samples as well healthy volunteers. This study included 286 Caucasian patients with arterial hypertension who received enalapril. concentrations and enalaprilat were determined before subsequent intake drug 4 h after it high-performance liquid chromatography...
Introduction . Epidermal growth factor receptor (EGFR) is a transmembrane protein of the tyrosine kinase family that activated in various cancers (non-small cell lung cancer, colorectal head and neck tumors). In glial brain tumors, increased EGFR expression levels are characteristic most aggressive subtype, glioblastoma. Frequent structural changes glioblastoma amplification chromosome region where gene located, point mutations, as well deletion exons 2–7 leading to formation EGFRvIII...
Gliomas are a heterogeneous group of brain tumors, among which the most aggressive subtype is glioblastoma, accounting for 60% cases in adults. Available systemic treatment options few and ineffective, so new approaches to therapies glioblastoma high demand. In total, 131 patients with diffuse glioma were studied. Paired tumor-normal samples sequenced on Illumina platform; panel included 812 genes associated cancer development. Molecular profiles clinically distinct groups investigated....
Favorable outcomes were achieved for children with acute lymphoblastic leukemia (ALL) the first Russian multicenter trial Moscow–Berlin (ALL-MB) 91. One major component of this regimen included a total 18 doses weekly intramuscular (IM) native Escherichia coli-derived asparaginase (E. coli-ASP) at 10000 U/m2 during three consolidation courses. ASP was initially available from Latvia, but had to be purchased abroad substantial costs after collapse Soviet Union. Therefore, subsequent ALL-MB...