A5047415599- Virus-based gene therapy research
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Single-cell and spatial transcriptomics
- Atmospheric aerosols and clouds
- interferon and immune responses
- Particle Dynamics in Fluid Flows
- Herpesvirus Infections and Treatments
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Aeolian processes and effects
- Viral gastroenteritis research and epidemiology
Ministry of Health of the Russian Federation
2024
Research Institute of Vaccines and Sera. Mechnikov of the Russian Academy of Medical Sciences
2022-2024
Glioblastoma cell lines derived from different patients are widely used in tumor biology research and drug screening. A key feature of glioblastoma is the high level inter- intratumor heterogeneity that accounts for treatment resistance. Our aim was to investigate whether maintained models. Single-cell RNA sequencing cellular composition a sample six patient-derived lines. Three preserved mutational profile original tumor, whereas three others differed their precursors. Copy-number variation...
Glioblastoma multiforme (GBM) accounts for almost half of all primary malignant brain tumors in adults and has a poor prognosis. Here we demonstrated the oncolytic potential L-16 vaccine strain measles virus (MV) against human GBM cells characterized genetic patterns that determine sensitivity to therapy. MV replicated cells, seven out eight cell lines underwent complete or partial oncolysis. RNA-Seq analysis identified about 1200 differentially expressed genes (FDR < 0.05) with at least...
This study evaluates the oncolytic potential of Moscow strain reovirus against human metastatic melanoma and glioblastoma cells. The effectively infects replicates within cell lines primary cells, while sparing non-malignant Infection leads to selective destruction neoplastic mediated by functional viral replication. A positive correlation was identified between RNA accumulation tumor death, with no replication observed in highlights critical roles cathepsins B, L, S as mediators process....
The sensitivity of human glioblastoma cells to virus-mediated oncolysis was investigated on five patient-derived cell lines. Primary (Gbl13n, Gbl16n, Gbl17n, Gbl25n, and Gbl27n) were infected with 10-fold serial dilutions the Leningrad-3 strain mumps virus, virus reproduction cytotoxicity monitored for 96–120 hours. Immortalized non-tumor NKE used as controls determine specificity. Four out lines examined susceptible infection, whereas no observed in line. Moreover, level proapoptotic...
Introduction. Oncolytic virotherapy is one of the approaches in immunotherapy solid brain tumors. Measles virus vaccine strains are prospective agents for therapy cancers such as neuroblastoma, mesothelioma, and glioblastoma multiforme. The hyperexpression CD46 other receptors on surface malignant cells allows measles to infect lyse tumor, thus inducing an immune response. However, widespread immunization population resistance neoplasms oncolysis present difficulties clinical practice. Text....