To study the isolates with acquired resistance to bedaquiline and linezolid that were obtained from patients enrolled in a clinical of novel therapy regimen for drug-resistant TB Moscow, Russia.Linezolid was detected using MGIT 960 critical concentration 1 mg/L. The MIC determined proportion method. identify genetic determinants resistance, sequencing mmpR ( Rv0678 ), atpE , atpC pepQ Rv1979c rrl rplC rplD loci performed.A total 85 27 reduced susceptibility (MIC ≥0.06 mg/L) tested. Most...

ABSTRACT Three new molecular approaches were developed to identify drug-resistant strains of Mycobacterium tuberculosis using biochips with oligonucleotides immobilized in polyacrylamide gel pads. These are significantly faster than traditional bacteriological methods. All three approaches—hybridization, PCR, and ligase detection reaction—were designed analyze an 81-bp fragment the gene rpoB encoding β-subunit RNA polymerase, where most known mutations rifampin resistance located. The call...

The goal of this work was to study the phenotypic susceptibility and resistance determinants N. gonorrhoeae isolates beta-lactam antimicrobials (benzylpenicillin ceftriaxone). A total 522 clinical collected in Russia 2015–2017 were analysed for using agar dilution method. DNA loci involved antimicrobial identified microarray analysis sequencing. Resistance benzylpenicillin remained high, with 7.7% resistant (MICpen > 1 mg/L) 47.5% showing intermediate = 0.12–1 mg/L). most frequent...

Early recognition of the risk Alzheimer's disease (AD) onset is a global challenge that requires development reliable and affordable screening methods for wide-scale application. Proteomic studies blood plasma are particular relevance; however, currently proposed differentiating markers poorly consistent. The targeted quantitative multiple reaction monitoring (MRM) assay reported candidate biomarkers (CBs) can contribute to creation consistent marker panel. An MRM-MS analysis 149 nondepleted...

Background: The gonococcal pathogen is capable of developing resistance to antimicrobials at a rapid rate. Aims: Updating data on the distribution genetic determinants isolates modern Russian population N. gonorrhoeae number antimicrobial drugs using hydrogel microarray technology. Materials and methods: study included 360 collected in Federal State Research Center Dermatovenereology Cosmetology 2019-2023. Results: In gonococcus, active processes are occurring that result redistribution...

Background/Objectives: The aim of this study was to evaluate the avidity thyroid autoantibodies (Abs) in sera patients with autoimmune disease (AITD) and autoantibody carriers without diagnosed AITD. Methods: A hydrogel microarray-based multiplex assay chaotrope destruction stage developed measure disease-associated autoantibodies, including those targeting thyroperoxidase (TPO), thyroglobulin (Tg), other minor antigens. Results: Evaluation three independent cohorts patients, totaling 266...

The search for concomitant autoimmune disorders (ADs) in patients with celiac disease is a pressing issue. study aimed to determine the rate of carrier state antibodies (Abs) being markers AD development using various immunological approaches. Enzyme-linked immunoassay and hydrogel microarray-based multiplex (MI) were used Abs against thyroid peroxidase (TPO), thyroglobulin (TG), glutamate decarboxylase (GAD), pancreatic islet cells (ICA), tyrosine phosphatase (IA2), 21-hydroxylase...

We describe a novel microarray-based approach for simultaneous identification and quantification of human immunodeficiency virus type 1 (HIV-1) hepatitis B C viruses (HBV HCV) in donor plasma specimens. The method is based on multiplex real-time RT-PCR performed within the microarray hydrogel pads. Double-stranded amplification products are simultaneously detected using nonspecific SYBR Green I dye due to reaction run separate pads bearing 5'-immobilized specific primers. Both sensitivity...

The steady rise in the spread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant (XDR-TB) requires rapid reliable methods to identify resistant strains. current molecular detect MTB resistance second-line drugs either do not cover an extended spectrum mutations be identified or are easily implemented clinical laboratories. A technique for detection M. has been developed using hybridisation analysis on microarrays. method allows identification within gyrA gyrB genes...

The widespread distribution of Neisseria gonorrhoeae strains that are resistant to previously used and clinically implemented antibiotics is a significant global public health problem. In line with WHO standards, the national Gonococcal Antimicrobial Surveillance Programme (RU-GASP) has been in existence Russia since 2004; herein, current status (2015) described, including associations between N. antimicrobial susceptibility, primary genetic resistance determinants specific strain sequence...

A microarray-based assay to detect IgG and IgM antibodies against betacoronaviruses (SARS-CoV-2, SARS, MERS, OC43, HKU1), other respiratory viruses type I interferons (IFN-Is) was developed. This multiplex applied track antibody cross-reactivity due previous contact with similar identify IFN-Is as the markers for severe COVID-19. In total, 278 serum samples from convalescent plasma donors, COVID-19 patients in intensive care unit (ICU) who recovered mild/moderate COVID-19, vaccine...

The diagnosis of autoimmune polyglandular syndrome (APS) types 1/2 is difficult due to their rarity and nonspecific clinical manifestations. APS-1 development can be identified with assays for autoantibodies against cytokines, APS-2 organ-specific antibodies. In this study, a microarray-based multiplex assay was proposed simultaneous detection both (anti-21-OH, anti-GAD-65, anti-IA2, anti-ICA, anti-TG, anti-TPO) APS-1-specific (anti-IFN-ω, anti-IFN-α-2a, anti-IL-22) autoantibodies. Herein,...

The emergence of multidrug resistance in N. gonorrhoeae is a serious global problem, and gonorrhea may soon become an incurable disease. aim the study was to characterize population Russia from 2015 2023 predict potential spread most concerning clones. A total 996 isolates were examined during analyzed period. Ceftriaxone azithromycin susceptibility testing performed using agar dilution method. Microarray-based assays sequencing employed identify genotypes genetic markers antimicrobial...

Immobilization of molecular probes in 3D hydrogel elements provides some essential advantages compared with conventional flat surfaces. In this article, an integrated technology based on the use low-density microarrays comprised hemispherical gel elements, developed at Engelhardt Institute Molecular Biology (Moscow, Russia) for various applications will be reviewed. The structure can adapted immobilization virtually any biological molecules a natural hydrophilic environment. discrimination...

Nucleic acid amplification tests are widely used in TB diagnostics. Priority tasks their development consist of increasing the specificity and sensitivity detection resistance to a wide spectrum anti-TB drugs. We developed multiplexed assay allowing 116 drug resistance-determining mutations rpoB, katG, inhA, ahpC, gyrA, gyrB, rrs, eis embB genes Mycobacterium tuberculosis complex genome six SNPs identify main lineages circulating Russia. The is based on 17 fragments using universal primer...

The Russian Gonococcal Antimicrobial Surveillance Programme (RU-GASP) was established in 2004 and operated continuously during the years from 2005 to 2016. aims of this study were summarize RU-GASP results over 12-year period evaluate trends

The aim of this work was to study the resistance macrolides (azithromycin) in modern Russian population N. gonorrhoeae with analysis genetic determinants. Azithromycin is not used treat gonococcal infection Russia. However, among 162 isolates collected 2020-2021, 22 (13.6%) were phenotypically resistant azithromycin. Mutations 23S rRNA genes found only two isolates; erm and mefA absent. shown be predominantly associated mutations mtrR mtrD MtrCDE efflux pump their mosaic alleles which may...

In addition to the obligatory pathogenic species of Mycobacterium tuberculosis complex and leprae, genus also includes conditionally that in rare cases can lead development nontuberculous mycobacterial diseases. Because mycobacteriosis have similar clinical signs, accurate identification causative agent a microbiology laboratory is important for diagnostic verification appropriate treatment. This report describes low-density hydrogel-based microarray containing oligonucleotide probes based...

The goal of this work was to determine the factors affecting emergence azithromycin-resistant Neisseria gonorrhoeae isolates in Russia, where azithromycin never recommended for treatment gonococcal infections. Clinical N. collected 2018-2021 (428 isolates) were analyzed. No found 2018-2019, but 2020-2021, a significant increase ratio observed: 16.8% and 9.3%, respectively. A hydrogel DNA microarray developed analysis resistance determinants: mutations genes encoding mtrCDE efflux system all...