A5014770973- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Molecular Research
- Polyamine Metabolism and Applications
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Biochemical Acid Research Studies
- Quinazolinone synthesis and applications
- Viral gastroenteritis research and epidemiology
- Amino Acid Enzymes and Metabolism
- DNA and Nucleic Acid Chemistry
- 3D Printing in Biomedical Research
- Click Chemistry and Applications
- Synthesis of heterocyclic compounds
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
Engelhardt Institute of Molecular Biology
2015-2024
Russian Academy of Sciences
2011-2022
Lomonosov Moscow State University
2022
Replication of hepatitis C virus (HCV) is associated with the induction oxidative stress, which thought to play a major role in various liver pathologies chronic C. NS5A protein one two key viral proteins that are known trigger production reactive oxygen species (ROS). To date it has been considered induces stress by altering calcium homeostasis. Herein we show NS5A‐induced was only moderately inhibited intracellular chelator BAPTA‐AM and not at all drug blocks Ca 2+ flux from ER...
Abstract In the absence of virus-targeting small-molecule drugs approved for treatment and prevention COVID-19, broadening repertoire potent SARS-CoV-2-neutralizing antibodies represents an important area research in response to ongoing pandemic. Systematic analysis such their combinations can be particularly instrumental identification candidates that may prove resistant emerging viral escape variants. Here, we isolated a panel 23 RBD-specific human monoclonal from B cells convalescent...
A microarray-based assay to detect IgG and IgM antibodies against betacoronaviruses (SARS-CoV-2, SARS, MERS, OC43, HKU1), other respiratory viruses type I interferons (IFN-Is) was developed. This multiplex applied track antibody cross-reactivity due previous contact with similar identify IFN-Is as the markers for severe COVID-19. In total, 278 serum samples from convalescent plasma donors, COVID-19 patients in intensive care unit (ICU) who recovered mild/moderate COVID-19, vaccine...
Severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and rapidly caused a pandemic that led to death >6 million people due hypercoagulation cytokine storm. In addition, SARS-CoV-2 triggers wide array pathologies, including liver dysfunction neurological disorders. It remains unclear if these events are direct infection respective tissues or result from systemic inflammation. Here, we explored possible hepatic CNS cell lines by SARS-CoV-2. We show...
HIV-induced immune suppression results in the high prevalence of HIV/AIDS-associated malignancies including Kaposi sarcoma, non-Hodgkin lymphoma, and cervical cancer. HIV-infected people are also at an increased risk “non-AIDS-defining” not directly linked to but associated with viral infections. Their incidence is increasing despite successful antiretroviral therapy. The mechanism behind this phenomenon remains unclear. Here, we obtained daughter clones murine mammary gland adenocarcinoma...
DNA vaccines require a considerable enhancement of immunogenicity. Here, we optimized prototype vaccine against drug-resistant HIV-1 based on weak Th2-immunogen, reverse transcriptase (RT). We designed expression-optimized genes encoding inactivated wild-type and RTs (RT-DNAs) introduced them into mice by intradermal injections followed electroporation. RT-DNAs were administered as single or double primes with without cyclic-di-GMP, prime boost RT-DNA mixed luciferase-encoding plasmid...
The fast spread of bacteria that are resistant to many classes antibiotics (multidrug resistant) is a global threat human and animal health with worrisome scenario ahead. Novel therapeutical strategies crucial importance combat this phenomenon. For purpose, we investigated the antimicrobial properties naturally occurring tripeptide Bialaphos dipeptide L-leucyl-L-phosphinoithricin, synthesis diastereomers separation which herein described. We demonstrate these compounds effective on clinical...
Hepatitis delta virus (HDV) is a viroid-like satellite that may co-infect individuals together with hepatitis B (HBV), as well cause superinfection by infecting patients chronic (CHB). Being defective virus, HDV requires HBV structural proteins for virion production. Although the encodes just two forms of its single antigen, it enhances progression liver disease to cirrhosis in CHB and increases incidence hepatocellular carcinoma. pathogenesis so far has been attributed virus-induced humoral...
Tumorigenesis is accompanied by the metabolic adaptation of cells to support enhanced proliferation rates and optimize tumor persistence amplification within local microenvironment. In particular, cancer exhibit elevated levels biogenic polyamines. Inhibitors polyamine biosynthesis inducers their catabolism have been evaluated as antitumor drugs, however, efficacy safety remain controversial. Our goal was investigate if drug-induced modulation metabolism plays a role in dedifferentiation...
Hepatitis C virus (HCV) is an oncogenic that causes chronic liver disease in more than 80% of patients. During the last decade, efficient direct acting antivirals were introduced into clinical practice. However, clearance does not reduce risk end-stage diseases to level observed patients have never been infected. So, investigation HCV pathogenesis still warranted. Virus-induced changes cell metabolism contribute development HCV-associated pathologies. Here we studied impact on polyamines and...
Hepatitis C virus (HCV) is an oncogenic that causes chronic liver disease in more than 80% of patients. During the last decade, efficient direct-acting antivirals were introduced into clinical practice. However, clearance does not reduce risk end-stage diseases to level observed patients who have never been infected. So, investigation HCV pathogenesis still warranted. Virus-induced changes cell metabolism contribute development HCV-associated pathologies. Here, we studied impact on...
Abstract Hepatitis C Virus (HCV) is a major public health problem worldwide. While highly efficacious directly-acting antiviral agents have been developed in recent years, their high costs and relative inaccessibility make use limited. Here, we describe new 1-(ω-phenoxyalkyl)uracils bearing acetanilide fragment 3 position of pyrimidine ring as potential drugs against HCV. Using combination various biochemical assays vitro virus infection replication models, show that our compounds are able...
A new series of potential nonnucleoside inhibitors HIV-1 reverse transcriptase (NNRTIs) based on the carbocyclic 5′-nor-uracil scaffold were designed and synthesized. Three different aspects investigated: effects adding a linker between phenyl fragments, introduction substituents benzoyl residue replacing central ring with benzyl group. Analogues 2′,3′-dideoxy-2′,3′-didehydro-5′-nor-uridine, bearing 3,5-dichloro- or 3,5-dimethylbenzoyl groups, showed inhibitory activity against HIV-RT...
A new series of potential nonnucleoside inhibitors HIV-1 reverse transcriptase (NNRTIs) based on the carbocyclic 5′-nor-uracil scaffold were designed and synthesized. Binding modes in active site enzyme studied computationally to provide insight interactions. Several 2′,3′-dideoxy-2′,3′-didehydro-5′-nor-uridine analogues showed inhibitory activity against wild-type mutant (L100I) HIV-RT with Ki 13–18 μM 1–11 μM, respectively.
Reactive oxygen species (ROS) play a major role in the regulation of various processes cell. The increase their production is factor contributing to development numerous pathologies, including inflammation, fibrosis, and cancer. Accordingly, study ROS neutralization, as well redox-dependent post-translational modifications proteins, warranted. Here, we present transcriptomic analysis gene expression redox systems related metabolic processes, such polyamine proline metabolism urea cycle...