A5018219936- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Spaceflight effects on biology
- Metabolomics and Mass Spectrometry Studies
- High Altitude and Hypoxia
- Analytical Chemistry and Chromatography
- Blood properties and coagulation
- Isotope Analysis in Ecology
- Advanced Chemical Sensor Technologies
- Muscle metabolism and nutrition
- Alzheimer's disease research and treatments
- Renal Diseases and Glomerulopathies
- Computational Drug Discovery Methods
- S100 Proteins and Annexins
- Pregnancy and preeclampsia studies
- Erythrocyte Function and Pathophysiology
- Analytical chemistry methods development
- Chronic Kidney Disease and Diabetes
- Crystallization and Solubility Studies
- Heart Rate Variability and Autonomic Control
- Biochemical effects in animals
- Microfluidic and Capillary Electrophoresis Applications
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Birth, Development, and Health
- Thermoregulation and physiological responses
Skolkovo Institute of Science and Technology
2016-2025
Semenov Institute of Chemical Physics
2019-2025
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation
2015-2024
Institute of Biochemical Physics NM Emanuel
2014-2024
Institute of Biomedical Problems
2016-2024
Independent University of Moscow
2024
Moscow Institute of Physics and Technology
2013-2022
Russian Academy of Sciences
2007-2022
Engelhardt Institute of Molecular Biology
2021-2022
Institute of Physics and Technology
2020
Mesenchymal stem cells (MSCs) have emerged as a potent therapeutic tool for the treatment of number pathologies, including immune pathologies. However, unwelcome effects MSCs on blood coagulation been reported, motivating us to explore thrombotic properties human from umbilical cord. We revealed strong procoagulant and platelet-free plasma using rotational thromboelastometry thrombodynamic tests. A similar potentiation clotting was demonstrated MSC-derived extracellular vesicles (EVs). To...
The objectives of this study were to identify molecular features characteristic arctic DOM from the Kolyma River basin and elucidate structural imprints induced by a choice sorption technique. To achieve goal, was isolated with use three nonionic sorbents: Amberlite XAD-8 resin, PPL- C18 - SPE cartridges, one anion exchanging resin-diethylaminoethyl (DEAE) -cellulose. studies conducted electrospray ionization Fourier Transform Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry liquid...
The detection of viral RNA by polymerase chain reaction (PCR) is currently the main diagnostic tool for COVID-19 ( Eurosurveillance 2019, 25 (3), 1). PCR-based test, however, shows limited sensitivity, especially in early and late stages disease development Nature 2020, 581, 465−469; J. Formosan Med. Assoc. 119 (6) 1123), relatively time-consuming. Fast reliable complementary methods detecting infection would be help current pandemic conditions. Mass spectrometry one such possibilities. We...
A simple method for hydrogen/deuterium exchange in a standard electrospray (ESI) ionization source is presented. In this method, D2O droplet placed between the ESI needle and entrance of mass spectrometer thus saturation atmosphere with deuterated vapor region achieved. It was shown that full up to 23 labile acidic hydrogens minimal back surrounding atmospheric water can be performed by method.
The effects of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in women on the gestation course and health fetus, particularly first second trimesters, remain very poorly explored. This report describes a case which normal development pregnancy was complicated immediately after patient had experienced Coronavirus disease 2019 (COVID-19) at 21st week gestation. Specific conditions included critical blood flow fetal umbilical artery, growth restriction (1st percentile),...
Mass spectrometry (MS)-based quantitative proteomic methods have become some of the major tools for protein biomarker discovery and validation. The recently developed parallel reaction monitoring-parallel accumulation–serial fragmentation (prm-PASEF) approach on a Bruker timsTOF Pro mass spectrometer allows addition ion mobility as new dimension to LC-MS-based proteomics increases proteome coverage at reduced analysis time. In this study, prm-PASEF was used multiplexed absolute quantitation...
The recent surge of coronavirus disease 2019 (COVID-19) hospitalizations severely challenges healthcare systems around the globe and has increased demand for reliable tests predictive severity mortality. Using multiplexed targeted mass spectrometry assays on a robust triple quadrupole MS setup which is available in many clinical laboratories, we determined precise concentrations hundreds proteins metabolites plasma from hospitalized COVID-19 patients. We observed clear distinction between...
The ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrum of natural organic matter (NOM) contains several thousand peaks with dozens molecules matching the same nominal mass. Such a complexity poses significant challenge for automatic data interpretation, in which most difficult task is molecular formula assignment, especially case heavy and/or multielement ions. In this study, new universal algorithm treatment FTICR spectra NOM and humic substances based on...
A method to enumerate labile hydrogens in all constituents of molecular ensemble natural organic matter (NOM) based on our previously developed simple hydrogen/deuterium (H/D) exchange (electrospray ionization (ESI) ion source (Kostyukevich et al. Anal. Chem. 2013, 85, 5330) and ultra-high-resolution Fourier transform cyclotron resonance mass spectrometry is presented. The was applied for analysis Suwannee River fulvic acid (SRFA), which an International Humic Substances Society standard, as...
We present the investigation of hydrogen/deuterium (H/D) exchange carbohydrates ions occurring in electrospray ion source. The shape deuterium distribution was observed to be considerably dependent on temperature transfer tube and solvent used. If deuterated alcohol (EtOD or MeOD) D2O/deuterated is used as an solvent, then for high temperatures (>350 °C), intensive back observed, resulting ∼30% depth exchange. At low (<150 weaker ∼70%. In intermediate region (∼250 unusually wide methanol...
The goal of this study was to establish a relationship between the optical properties soil dissolved organic matter (DOM) and acidic functions carried out by its individual constituents. We obtained 12 fractions DOM samples using sequential solid phase extraction on nonionic sorbent at steadily lowered pH values: 7, 5, 3, 2, which correspond low bounds pKa values phenols, aliphatic, aromatic carboxylic acids, ketoacids. structural studies were conducted with use NMR selective...
Abstract Aim Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are podocytopathies characterized by damage to the glomerular filtration barrier, leading proteinuria nephrotic syndrome. The production of anti‐podocyte antibodies has been proposed as potential circulating factors contributing development these conditions. aim study is evaluate levels anti‐nephrin in patients with healthy subjects. Methods In this study, a total 77 active glomerulopathy 11 subjects were...
The exact structural formulas of model humic substances are identified using controllable H/D exchanged coupled to FTMS.
In the paper, we have demonstrated possibility of performing hydrogen/deuterium (H/D) exchange proteins in region gas‐phase ion formation an electrospray source by saturating ionization with vapors a deuterating agent (D 2 O or MeOD). this region, charged droplets are shrinking and protein ions transfer into gas phase. As model protein, used ubiquitin whose mobility spectrometry H/D vacuum part mass spectrometer presence conformers different cross sections rates. our experiments, observed...
Recent findings indicate that the ubiquitin-proteasome system is involved in pathogenesis of cancer as well autoimmune and several neurodegenerative diseases, thus a target for novel therapeutics. One disease related to aberrant protein degradation multiple sclerosis, an disorder involving processing presentation myelin autoantigens leads destruction axons. Here, we show brain-derived proteasomes from SJL mice with experimental encephalomyelitis (EAE) ubiquitin-independent manner generate...
Early recognition of the risk Alzheimer's disease (AD) onset is a global challenge that requires development reliable and affordable screening methods for wide-scale application. Proteomic studies blood plasma are particular relevance; however, currently proposed differentiating markers poorly consistent. The targeted quantitative multiple reaction monitoring (MRM) assay reported candidate biomarkers (CBs) can contribute to creation consistent marker panel. An MRM-MS analysis 149 nondepleted...
The analysis of exhaled breath condensate (EBC) can be an alternative to traditional endoscopic sampling lower respiratory tract secretions. This is a simple non-invasive method diagnosing diseases, in particular, inflammatory processes.Samples were collected with special device-condenser (ECoScreen, VIASYS Healthcare, Germany), then treated trypsin according the proteomics protocol for standard protein mixtures and analyzed by nanoflow high-performance liquid chromatography tandem mass...
Abstract Oxidative and nitrosative stress leaves footprints in the plant chloroplast form of oxidatively modified proteins. Using a mass spectrometric approach, we identified 126 tyrosine 12 tryptophan nitration sites 164 nitrated proteolytic peptides, mainly from photosystem I (PSI), II (PSII), cytochrome b 6 /f ATP‐synthase complexes 140 oxidation products tyrosine, tryptophan, proline, phenylalanine histidine residues. While high number were found proteins four photosynthetic indicating...
Long-term space travel simulation experiments enabled to discover different aspects of human metabolism such as the complexity NaCl salt balance. Detailed proteomics data were collected during Mars105 isolation experiment enabling a deeper insight into molecular processes involved. We studied abundance about two thousand proteins extracted from urine samples six volunteers weekly 105-day under controlled dietary conditions including progressive reduction consumption. Machine learning using...
Prebiotic peptide formation under aqueous conditions in the presence of metal ions is one plausible triggers emergence life. The salt-induced reaction has been suggested as being prebiotically relevant and was examined for peptides NaCl solutions. In previous work we have argued that first protocell could emerged KCl solution. Using HPLC-MS/MS analysis, found K+ more than an order magnitude effective L-glutamic acid oligomerization with 1,1'-carbonyldiimidazole solutions same concentration...