A5000127669- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Effects and risks of endocrine disrupting chemicals
- Advanced Memory and Neural Computing
- Laser-Matter Interactions and Applications
- Remote Sensing and Land Use
- Spectroscopy and Quantum Chemical Studies
- Berry genetics and cultivation research
- Receptor Mechanisms and Signaling
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Estrogen and related hormone effects
- Safety and Risk Management
- Cardiac Fibrosis and Remodeling
- Mass Spectrometry Techniques and Applications
- Granular flow and fluidized beds
- Nausea and vomiting management
- Reproductive Physiology in Livestock
- Landslides and related hazards
- Mitochondrial Function and Pathology
- Cardiac Ischemia and Reperfusion
- Computational Drug Discovery Methods
- Advanced Chemical Physics Studies
- Liver physiology and pathology
China Medical University
2014-2025
Hangzhou Normal University
2023-2024
Zhejiang Normal University
2023-2024
Kunming University of Science and Technology
2024
Dalian Institute of Chemical Physics
2023
Chinese Academy of Sciences
2023
Jilin University
2019-2021
Kagoshima University
2017-2020
Institute of High Energy Physics
2020
Harbin Institute of Technology
2019-2020
Abstract Introduction Lower respiratory tract infections(LRTIs) in adults are complicated by diverse pathogens that challenge traditional detection methods, which often slow and insensitive. Metagenomic next-generation sequencing (mNGS) offers a comprehensive, high-throughput, unbiased approach to pathogen identification. This retrospective study evaluates the diagnostic efficacy of mNGS compared conventional microbiological testing (CMT) LRTIs, aiming enhance accuracy enable early clinical...
Abstract Objective Dysfunction of calcium/calmodulin (CaM)–dependent kinase II (CaMKII) has been involved in hyperexcitability‐related disorders including epilepsy. However, the relationship between CaMKII and neuronal excitability remains unclear. Methods Neuronal was detected vivo vitro by electroencephalography (EEG), patch clamp multi‐electrode array (MEA), respectively. Next, we assessed currents voltage‐gated sodium channels (VGSCs) clamp, mRNA protein expressions VGSCs were determined...
Photodissociation of CO2 via the 1Πg state is investigated using a time-sliced velocity-mapped ion imaging apparatus combined with tunable vacuum ultraviolet photolysis source. The main O(1D2) + CO(X1Σ+) channel directly observed from measured images photoproducts at 129.08–134.76 nm. total kinetic energy release spectra determined based on these show that energetic thresholds for correspond to thermochemical photodissociation CO2(v2 = 0) and 1). One significant difference among CO(X1Σ+, v)...
Objectives . Inhibition of calcium‐/calmodulin‐ (CaM‐) dependent kinase II (CaMKII) is correlated with epilepsy. However, the specific mechanism that underlies learning and memory impairment neuronal death by CaMKII inhibition remains unclear. Materials Methods In this study, KN93, a inhibitor, was used to investigate role during epileptogenesis. We first identified differentially expressed genes (DEGs) in primary cultured hippocampal neurons or without KN93 treatment using RNA‐sequencing....
Activity of cardiac Cav1.2 channels is enhanced by cyclic AMP-PKA signaling. In this study, we studied the effects PKA phosphorylation on binding calmodulin to fragment peptide proximal C-terminal tail α1C subunit (CT1, a.a. 1509–1789 guinea-pig). pull-down assay, in vitro significantly decreased CT1 (61%) at high [Ca2+]. The phosphoresistant (CT1SA) and phosphomimetic (CT1SD) mutants, which three sites (Ser1574, 1626, 1699) were mutated Ala Asp, respectively, bound with 99% 65% amount,...
The present study examined the binding of individual N‐ and C‐lobes calmodulin (CaM) to Cav1.2 at different Ca 2+ concentration ([Ca ]) from ≈ free 2 mM, found that they may bind ‐dependently. In particular, using patch‐clamp technique, we confirmed or can rescue basal activity run‐down, demonstrating functional relevance lobes. data imply resting [Ca ], CaM tether channel with its single lobe, leading multiple molecule increase grade ‐dependent regulation Cav1.2.
To demonstrate the interaction of calpastatin (CS) domain L (CS ) with Cav1.2 channel, we investigated binding CS various C‐terminus‐derived peptides at ≈ free, 100 nM, 10 μM, and 1 mM Ca 2+ by using GST pull‐down assay method. Besides IQ motif, was also found to bind PreIQ motif. With increasing [Ca ], affinity –IQ gradually decreased, –PreIQ increased. The results suggest that may both motifs channel in different ‐dependent manners.
To investigate the efficacy of ropivacaine and bupivacaine in caesarean section vital signs hemodynamics lying-in women.A total 480 women who were admitted to this hospital for treatment between December 2017 June 2018 enrolled into study as subjects, which divided experiment group control group, with 240 subjects each group. In received local anesthesia by infusion 1.5 mL (0.75%), while those also took (0.75%). Thereafter, we observed differences anesthetic efficiency, two groups.The...
Dilated cardiomyopathy (DCM) is a primary cause of heart failure (HF), with the incidence HF increasing consistently in recent years. DCM pathogenesis involves combination inherited predisposition and environmental factors. Endocrine-disrupting chemicals (EDCs) are exogenous that interfere endogenous hormone action capable targeting various organs, including heart. However, impact these disruptors on disease through their effects genes remains underexplored. In this study, we aimed to...
Previous studies using citrin/mitochondrial glycerol-3-phosphate (G3P) dehydrogenase (mGPD) double-knockout mice have demonstrated that increased dietary protein reduces the extent of carbohydrate-induced hyperammonemia observed in these mice. This study aimed to further elucidate mechanisms this effect. Specific amino acids were initially found decrease hepatic G3P, or increase aspartate citrulline levels, mGPD-knockout administered ethanol. Unexpectedly, oral glycine ammonia addition...
Cardiac Cav1.2 channels, coupling membrane stimulation to intracellular Ca2+ signaling, are regulated by multiple cytoplasmic factors, such as calmodulin (CaM), phosphorylation, Ca2+, ATP and intramolecular fragments of the channel. The interaction between distal proximal C-terminal regulatory domains (DCRD PCRD) channel is suggested inhibit activity, while PKA-mediated phosphorylation facilitates releasing an interaction. Here, we report that C-terminus (CT3) (CT1) inhibited CaM in a...
Culture of hippocampal neurons in low-Mg 2+ medium (low-Mg neurons) results induction continuous seizure activity. However, the underlying mechanism contribution low Mg to hyperexcitability has not been clarified. Our data, obtained using patch-clamp technique, show that voltage-gated Na + channel (VGSC) activity, which is associated with a persistent, noninactivating current ( I Na,P ), was modulated by calmodulin (CaM) concentration-dependent manner normal and neurons, but activity more...
To understand the mechanism underlying regression of cardiac hypertrophy, we investigated pathological changes after isoproterenol (ISO) withdrawal in ISO-induced cardiomyopathy models rats and neonatal cardiomyocytes. Cardiac hypertrophy was induced by two weeks ISO administration; however, did not regress three natural maintenance administration withdrawn (ISO-wdr group). The remaining ISO-wdr group accompanied a sustained increase level phosphorylated Ca2+/calmodulin-dependent protein...
The decay dynamics of 2-aminopyridine and 3-aminopyridine excited to the S1 state is investigated using femtosecond time-resolved photoelectron imaging. lifetime for both molecules shows a rapid decrease with increase vibrational energy. It shown that, besides intersystem crossing lower-lying triplet T1, ground (S0) via internal conversion through conical intersection plays an increasingly important role becomes dominant states well above origin. comparison between suggests that...
Nonylphenol (NP), a widely distributed, toxic, endocrine-disrupting chemical, has estrogenic properties. However, its cardiac effects remain unclear. In this study, the of NP on isolated guinea pig hearts were studied in three separate experiments. First, perfused with 10-7 M or 10-5 to determine whether was toxic healthy hearts. Next, subjected 50 min ischemia and 60 reperfusion (I50R60) could aggravate ischemia/reperfusion (I/R) injury. Finally, interaction cardio-protective agent...