A5034763598- Neuroinflammation and Neurodegeneration Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Polyamine Metabolism and Applications
- Neuroscience and Neuropharmacology Research
- Blood properties and coagulation
- Amino Acid Enzymes and Metabolism
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Neurogenesis and neuroplasticity mechanisms
- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Metabolism and Genetic Disorders
- Adenosine and Purinergic Signaling
- Eicosanoids and Hypertension Pharmacology
- Cannabis and Cannabinoid Research
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Drug Transport and Resistance Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Sphingolipid Metabolism and Signaling
- Prion Diseases and Protein Misfolding
- Pancreatitis Pathology and Treatment
- Neurological Disease Mechanisms and Treatments
- Diet and metabolism studies
- Sirtuins and Resveratrol in Medicine
Osaka Prefecture University
2012-2021
Kanazawa University
2003-2007
Kanazawa Medical University
2005-2007
To determine the role of endoplasmic reticulum (ER) in diabetes, Akita mice, a mouse model type 2 were mated with either heterozygous knockout mice or two types transgenic 150-kDa oxygen-regulated protein (ORP150), molecular chaperone located ER. Systemic expression ORP150 improves insulin intolerance, whereas exclusive overexpression pancreatic beta-cells did not change their glucose tolerance. Both an tolerance test and hyperinsulinemic-euglycemic clamp revealed that enhanced uptake,...
Enhanced endoplasmic reticulum (ER) stress has been implicated in various pathological situations including inflammation. During a search for compounds that regulate ER stress, we identified vaticanol B, tetramer of resveratrol, as an agent protects against stress-induced cell death. Vaticanol B suppressed the induction unfolded protein response-targeted genes such glucose-regulated 78 (GRP78) and C/EBP-homologous (CHOP) after cells were treated with stressors. Analysis mouse macrophage line...
AbstractStress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome-associated membrane 4 (RAMP4), is a Sec61-associated polypeptide that induced by ER stress. SERP1−/− mice, made targeted gene disruption, demonstrated growth retardation, increased mortality, and impaired glucose tolerance. Consistent with high levels of SERP1 expression in pancreas, pancreatic islets from mice failed to rapidly synthesize proinsulin response load. In addition, reduced size...
Enhanced endoplasmic reticulum (ER) stress leads to cell death in various pathophysiological situations. During a search for compounds that regulate ER stress, we identified methoxyflavones, group of flavonoids, as strong protective agents against stress. Analysis mouse insulinoma MIN6 cells revealed methoxyflavones mildly activated the eukaryotic initiation factor 2α and nuclear erythroid 2-related pathways, but not XBP1 pathway, induced downstream genes, including glucose-regulated protein...
The enhancement of intracellular stresses such as oxidative stress and endoplasmic reticulum (ER) has been implicated in several neurodegenerative disorders including Parkinson's disease (PD). During a search for compounds that regulate ER stress, dibenzoylmethane (DBM) derivative 14-26 (2,2′-dimethoxydibenzoylmethane) was identified novel neuroprotective agent. Analysis SH-SY5Y cells PC12 revealed the regulation by associated with its anti-oxidative property. prevented production reactive...
Abstract In cortical neurons cultured for 3 or 9 days in vitro (DIV), exposure to hydrogen peroxide (H 2 O ) led a marked decrease cell viability concentration‐dependent manner at concentration range of 10 µ m 1 irrespective the duration between 6 and 24 h. However, H was more potent decreasing cellular DIV than those DIV. Pyruvate effective preventing neuronal death even when added 1–3 h after addition . Semi‐quantitative RT–PCR western blotting analyses revealed significantly higher...
<b><i>Objectives:</i></b> In peripheral macrophages, tissue-type transglutaminase (TG2) is reported to be involved in phagocytosis of apoptotic cells. However, the contribution TG2 microglial has not been investigated. this study, using a cell line, BV-2, we examined changes expression, and pinocytosis cells stimulated by lipopolysaccharide (LPS). <b><i>Methods:</i></b> Cells mouse line BV-2 were LPS with or without cystamine, an inhibitor TG...
Cumulative evidence indicates that estrogen receptor (ER) agonists attenuate neuroinflammation. Equol, a major isoflavone from soybean, exhibits estrogen-like biological activity, but their effect on inflammatory response has not been well established. Here, we investigated the of S -equol nitric oxide (NO) production, well-known change in astrocytes stimulated by LPS. -Equol attenuated LPS-induced NO production with concomitant decrease expression inducible synthase (iNOS). did affect...
Mesenchymal stem/stromal cells (MSCs) have been used as cell sources for treating dogs with naturally-occurring diseases. Extracellular vesicles (EVs) derived from MSCs are now recognized pivotal to modulating the immune response and supporting tissue repair. Manufacture of MSC-EVs clinical application mandates removal xeno-proteins, including fetal bovine serum. The objective this study was examine whether canine survived secreted EVs in serum-free medium (SFM) conditions assess...
Abstract The prevailing view is that the glutamine (Gln) transporter (GlnT/ATA1/SAT1/SNAT1) a member of system A superfamily with ability to fuel glutamate/Gln cycle at nerve terminals in glutamatergic neurons. Semiquantitative reverse transcription‐polymerase chain reaction revealed similarly high expression mRNA for GlnT by rat brain neocortical astrocytes as well neurons, progressively lower cerebellar astrocytes, hippocampal and whole‐brain microglia culture. [ 3 H]Gln was accumulated...
Previous studies using citrin/mitochondrial glycerol-3-phosphate (G3P) dehydrogenase (mGPD) double-knockout mice have demonstrated that increased dietary protein reduces the extent of carbohydrate-induced hyperammonemia observed in these mice. This study aimed to further elucidate mechanisms this effect. Specific amino acids were initially found decrease hepatic G3P, or increase aspartate citrulline levels, mGPD-knockout administered ethanol. Unexpectedly, oral glycine ammonia addition...
MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and its metabolite 1-methyl-4-phenylpyridinium (MPP+) are drugs that widely used in experimental Parkinson disease (PD) models. What is the significance of ORP150/HSP12A, a molecular chaperone endoplasmic reticulum (ER), nigrostriatal system? Dopaminergic neuroblastoma SH-SY5Y cells dopaminergic neurons substantia nigra pars compacta (SNpc) were examined. Our observations led to hypothesis ORP150 protects against MPTP/MPP+-induced...