A5039220176- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Neurotransmitter Receptor Influence on Behavior
- Regulation of Appetite and Obesity
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Hypothalamic control of reproductive hormones
- EEG and Brain-Computer Interfaces
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Stress Responses and Cortisol
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Drug Transport and Resistance Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Genetic Neurodegenerative Diseases
- Ion channel regulation and function
- Metabolism and Genetic Disorders
- Advanced Fluorescence Microscopy Techniques
- Adipose Tissue and Metabolism
Kagoshima University
2014-2024
ORCID
2021
Tokyo Denki University
2015
Kanazawa University
2004-2012
Kobe University
2012
Hirosaki University
2006
Gunma Children's Medical Center
1986
L-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- intra-cellular dynamics L-lactate are currently hampered by limited selection performance L-lactate-specific genetically encoded biosensors. Here we now report spectrally functionally orthogonal pair high-performance biosensors: green fluorescent extracellular biosensor, designated eLACCO2.1, red intracellular R-iLACCO1. eLACCO2.1 exhibits excellent membrane...
Midbrain dopamine neurons impact neural processing in the prefrontal cortex (PFC) through mesocortical projections. However, signals conveyed by projections to PFC remain unclear, particularly at single-axon level. Here, we investigated dopaminergic axonal activity medial (mPFC) during reward and aversive processing. By optimizing microprism-mediated two-photon calcium imaging of axon terminals, found diverse axons responsive both stimuli. Some exhibited a preference for reward, while others...
Abstract Notoginsenoside R1 (NTR1) is the main active ingredient in Panax notoginseng , a herbal medicine widely used Asia for years. The purpose of this study was to investigate pharmacological properties NTR1 on neurotoxicity glutamate (Glu) primary cultured mouse cortical neurons along with its possible mechanism action. Wefound that significantly protected from loss cellular viability caused by brief exposure 10 μM Glu 1 hr dose‐dependent manner at concentrations 0.1 μM, without...
C-type natriuretic peptide (CNP) is abundant in brain and reported to exert autocrine function vascular cells, but its effect on blood–brain barrier (BBB) permeability has not been clarified yet. Here, we examined this effect. Transendothelial electrical resistance (TEER) of vitro BBB model, composed bovine microvascular endothelial cells astrocytes, was significantly dose dependently decreased by CNP (1, 10, 100 nmol/L). treatment reduced both the messenger RNA (mRNA) protein expressions...
Abstract Three types of bi‐functionalized copolymers ( P1FAz , P2FAz and P3FAz ) with different numbers fluorene units an azobenzene unit were synthesized characterized using UV–vis polarized absorption spectroanalysis. The trans‐cis photoisomerization was conformed under 400 nm light irradiation for all in chloroform. However, the film state, only trans ‐ cis occurred by mono‐fluorene attached copolymer poly[(9,9‐di‐ n ‐octylfluorenyl‐2,7‐diyl)‐ alt ‐4,4′‐azobenzene)] ). Photo‐induced...
Dopamine neurons play crucial roles in pleasure, reward, memory, learning, and fine motor skills their dysfunction is associated with various neuropsychiatric diseases. receptors are the main target of treatment for neurologic psychiatric disorders. Antipsychotics that antagonize dopamine D2 receptor (DRD2) used to alleviate symptoms these disorders but may also sometimes cause disabling side effects such as parkinsonism (catalepsy rodents). Here we show GPR143, a G-protein-coupled...
Abstract Neuronal nicotinic acetylcholine receptors (nAChRs) are composed of an assembly between at least seven alpha (α2–α7, α9) and three beta (β2–β4) subunits in mammals. The addition 50 mM KCl or 1 nicotine immediately increased the number cells with high fluorescence intensity rat cortical astrocytes on fluo‐3 measurement. Nicotine was effective increasing cultured for 2 days after replating, but not those used 5 without markedly affecting cellular viability irrespective exposure...
Abstract In cortical neurons cultured for 3 or 9 days in vitro (DIV), exposure to hydrogen peroxide (H 2 O ) led a marked decrease cell viability concentration‐dependent manner at concentration range of 10 µ m 1 irrespective the duration between 6 and 24 h. However, H was more potent decreasing cellular DIV than those DIV. Pyruvate effective preventing neuronal death even when added 1–3 h after addition . Semi‐quantitative RT–PCR western blotting analyses revealed significantly higher...
Intrathecal (i.t.) administration of pituitary adenylate cyclase-activating polypeptide (PACAP) induces long-lasting nociceptive behaviors for more than 60 min in mice, while the involvement PACAP type1 receptor (PAC1-R) has not been clarified yet. The present study investigated signaling mechanisms PACAP-induced prolonged behaviors. Single i.t. injection a selective PAC1-R agonist, maxadilan (Max), mimicked dose-dependent manner similar to PACAP. Pre- or post-treatment antagonist, max.d.4,...
Abstract We have attempted to elucidate mechanisms underlying differential vulnerability glutamate (Glu) using cultured neurons prepared from discrete structures of embryonic rat brains. Brief exposure Glu led a significant decrease in the mitochondrial activity hippocampal for 9 or 12 days at 10 μM 1 mM with an apoptosis‐like profile, without markedly affecting that cortical neurons. also increased lactate dehydrogenase release along marked number cells immunoreactive neuronal marker...
Abstract In rat hippocampal neurons cultured with the antagonist for N‐methyl‐D‐aspartate (NMDA) receptors dizocilpine (MK‐801) 8 days in vitro (DIV), a significant decrease was seen expression of microtubule‐associated protein‐2 (MAP‐2) as well mRNA both brain‐derived neurotrophic factor (BDNF) and growth‐associated protein‐43 (GAP‐43), addition to decreased viability. MK‐801 not only NR1 subunit NMDA but also increased NR2A expression, without affecting NR2B expression. Repetitive daily...
Abstract The prevailing view is that the glutamine (Gln) transporter (GlnT/ATA1/SAT1/SNAT1) a member of system A superfamily with ability to fuel glutamate/Gln cycle at nerve terminals in glutamatergic neurons. Semiquantitative reverse transcription‐polymerase chain reaction revealed similarly high expression mRNA for GlnT by rat brain neocortical astrocytes as well neurons, progressively lower cerebellar astrocytes, hippocampal and whole‐brain microglia culture. [ 3 H]Gln was accumulated...
The initiation of goal-directed actions is a complex process involving the medial prefrontal cortex and dopaminergic inputs through mesocortical pathway. However, it unclear what information pathway conveys how impacts action initiation. In this study, we unveiled indispensable role axon terminals in encoding execution movements self-initiated actions.
We have previously shown differential vulnerabilities to glutamate (Glu) excitotoxicity mediated by the N-methyl-D-aspartate (NMDA) receptor (NMDAR) between rat cortical and hippocampal neurons in culture. In this study, we evaluated possible induced tolerance NMDA neurotoxicity cultured striatal with prior sustained activation of NMDAR. Brief exposure Glu or for 1 hr led a significant decrease cellular vitality determined 24 later neurons, whereas no marked loss was seen survival after...