A5058155846- Receptor Mechanisms and Signaling
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- nanoparticles nucleation surface interactions
- Icing and De-icing Technologies
- Arctic and Antarctic ice dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- bioluminescence and chemiluminescence research
- 3D Printing in Biomedical Research
- Photochromic and Fluorescence Chemistry
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Machine Learning in Materials Science
- Gene Regulatory Network Analysis
- Antimicrobial Peptides and Activities
The University of Tokyo
2021-2024
University of Alberta
2018
Abstract l -Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles shuttling -lactate, we now report intensiometric green fluorescent genetically encoded biosensor for extracellular -lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging -lactate cultured mammalian cells and brain tissue.
L-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- intra-cellular dynamics L-lactate are currently hampered by limited selection performance L-lactate-specific genetically encoded biosensors. Here we now report spectrally functionally orthogonal pair high-performance biosensors: green fluorescent extracellular biosensor, designated eLACCO2.1, red intracellular R-iLACCO1. eLACCO2.1 exhibits excellent membrane...
L-Lactate, once considered a metabolic waste product of glycolysis, is now recognized as vitally important metabolite and signaling molecule in multiple biological pathways. However, exploring L-lactate's emerging intra- extra-cellular roles hindered by lack tools to perturb L-lactate concentration intracellularly extracellularly. Photocaged compounds are powerful way introduce bioactive molecules with spatiotemporal precision using illumination. Here, we report the development photocaged...
Abstract L-Lactate, traditionally recognized as a waste product of metabolism, is now appreciated key intercellular energy currency in mammals. To enable investigations shuttling L-lactate, we have previously reported eLACCO1.1, green fluorescent genetically encoded biosensor for extracellular L-lactate. eLACCO1.1 enables cellular resolution imaging L-lactate cultured mammalian cells and brain tissue. However, spectrally overlaps with commonly used optical biosensors actuators, limiting its...
Abstract l -Lactate, once considered a metabolic waste product of glycolysis, is now recognized as vitally important metabolite and signaling molecule in multiple biological pathways. However, exploring -lactate’s emerging intra- extra-cellular roles hindered by lack tools to locally perturb -lactate concentration intracellularly extracellularly. Photocaged compounds are powerful way introduce bioactive molecules with spatial temporal precision using illumination. Here, we report the...
In this manuscript, we developed a screening system that employs the difference in density between liquid water and ice (0.9998 g/cm3 vs 0.9168 at 0 °C) to identify ice-nucleating agents (INAs) are encapsulated into droplets of suspended silicone oil intermediate (0.939 g/cm3). Droplets stably reside interface perfluoro (1.6658 g/cm3); freezing causes aqueous float top layer. We demonstrated feasibility by using contained well-defined ice-nucleator Snomax. The with without Snomax froze...
<title>Abstract</title> The transient build-up of L-lactate in tissue is associated with acidosis, as occurs during hypoxia, and traditionally viewed deleterious1–3. However, recent studies contradict this view by demonstrating L-lactate's vital role an intercellular interorgan exchangeable fuel4. As one example, the astrocyte-to-neuron lactate shuttle (ANLS) hypothesis proposes that neurons import from astrocytes via extracellular space to produce adenosine triphosphate (ATP) sustain...
Abstract To enable investigations of the emerging roles cell-to-cell shuttling L-lactate, we have developed an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. We demonstrate that this biosensor, designated eLACCO1.1, enables minimally invasive cellular resolution imaging L-lactate in cultured mammalian cells and brain tissue.
Abstract l -Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. To enable investigations of both the inter- intra-cellular dynamics -Lactate, we develop second-generation green fluorescent extracellular biosensor, designated eLACCO2.1, red intracellular R-iLACCO1. Compared to first-generation eLACCO1.1 (Δ F / = 1.5 cultured neurons), eLACCO2.1 exhibits better membrane localization fluorescence response 8.1 neurons) with faster kinetics on surface live...
In this publication, we developed the high throughput screening implementation of freeze-float selection platform system established in previous publication. The goal publication is to expand higher and accuracy. following sections, describe steps for automated droplets generations, adaptation using controlled uniform temperature cooling system, semi-automated detection program. We aimed improve previously published add functional advantages, such as; a) increased efficiency with fewer...
In this publication, we developed the high throughput screening implementation of freeze-float selection platform system established in previous publication. The goal publication is to expand higher and accuracy. following sections, describe steps for automated droplets generations, adaptation using controlled uniform temperature cooling system, semi-automated detection program. We aimed improve previously published add functional advantages, such as; a) increased efficiency with fewer...