A5017451231- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Neuropeptides and Animal Physiology
- Neurotransmitter Receptor Influence on Behavior
- Pain Mechanisms and Treatments
- Regulation of Appetite and Obesity
- melanin and skin pigmentation
- Sleep and Wakefulness Research
- Nitric Oxide and Endothelin Effects
- Nicotinic Acetylcholine Receptors Study
- Olfactory and Sensory Function Studies
- Pulmonary Hypertension Research and Treatments
- Sleep and related disorders
- Neuroscience of respiration and sleep
- Pain Management and Placebo Effect
- Pharmacological Receptor Mechanisms and Effects
- Axon Guidance and Neuronal Signaling
- Nuclear Receptors and Signaling
- Biotin and Related Studies
- Photoreceptor and optogenetics research
- Circadian rhythm and melatonin
- Parkinson's Disease Mechanisms and Treatments
- Ion Channels and Receptors
- Pancreatic and Hepatic Oncology Research
Yokohama City University
2015-2024
Kōchi University
2022
Hoshi University
2013-2020
Ebara (Japan)
2014-2016
Tokyo University of Science
2013
Josai International University
2013
Reducing the side effects of pain treatment is one most important strategies for improving quality life cancer patients. However, little known about mechanisms that underlie these effects, especially constipation induced by opioid receptor agonists; i.e., do they involve naloxonazine-sensitive versus -insensitive sites or central-versus-peripheral μ-opioid receptors? The present study was designed to investigate agonist-induced (i.e., inhibition gastrointestinal transit and colonic...
Dopamine neurons play crucial roles in pleasure, reward, memory, learning, and fine motor skills their dysfunction is associated with various neuropsychiatric diseases. receptors are the main target of treatment for neurologic psychiatric disorders. Antipsychotics that antagonize dopamine D2 receptor (DRD2) used to alleviate symptoms these disorders but may also sometimes cause disabling side effects such as parkinsonism (catalepsy rodents). Here we show GPR143, a G-protein-coupled...
L-DOPA is generally considered to alleviate the symptoms of Parkinson's disease by its conversion dopamine. We have proposed that DOPA itself a neurotransmitter in CNS. However, specific receptors for not been identified. Recently, gene product ocular albinism 1 (OA1) was found exhibit DOPA-binding activity. Here, we investigated whether OA1 functional receptor nucleus tractus solitarii (NTS).We examined immunohistochemical expression NTS, and effects microinjected into depressor sites NTS...
Abstract Dopamine (DA) is involved in neurological and physiological functions such as motor control. L‐3,4‐dihydroxyphenylalanine (L‐DOPA), a precursor of DA, conventionally believed to be an inert amino acid its major therapeutic effects Parkinson's disease (PD) are mediated through conversion DA. On the contrary, accumulating evidence suggests that L‐DOPA itself neurotransmitter. We here show potentiates DA D2 receptor (DRD2) signaling GPR143, gene product X‐linked ocular albinism 1,...
Blood pressure is regulated by extrinsic factors including noradrenaline, the sympathetic neurotransmitter that controls cardiovascular functions through adrenergic receptors. However, fine-tuning system of noradrenaline signaling relatively unknown. We here show l-3,4-dihydroxyphenylalanine (L-DOPA), a precursor catecholamines, sensitizes vascular receptor alpha1 (ADRA1) activation L-DOPA GPR143. In WT mice, intravenous infusion ADRA1 agonist phenylephrine induced transient elevation blood...
Abstract The roles of serotonergic and noradrenergic signaling in nociceptive processing the central nervous system are well known. However, dopaminergic is also relevant to various physical functions, including nociception. zona incerta a subthalamic nucleus which A13 cell group resides, but how this affects remains unknown. Recently, we showed that acute stimuli rapidly induce activity A10 (ventral tegmental area) dopamine neurons via fiber photometry. In study, measured response using...
Alzheimer's disease (AD) is an incurable neurodegenerative characterized by memory loss and neurotoxic amyloid beta (Aβ) plaques accumulation. Numerous pharmacological interventions targeting Aβ accumulation have failed to alleviate AD. Also, the pathological alterations in AD start years before onset of clinical symptoms. To identify proteins at play during early stage AD, we conducted proteomic analysis hippocampus young AppNL-F mice model preclinical phase disease. This was followed...
Abstract Neurogenesis occurs in the hippocampus throughout life and is implicated various physiological brain functions such as memory encoding mood regulation. L-3,4-dihydroxyphenylalanine (L-DOPA) has long been believed to be an inert precursor of dopamine. Here, we show that L-DOPA its receptor, GPR143, gene product ocular albinism 1, regulate neurogenesis dentate gyrus (DG) a dopamine-independent manner. at concentrations far lower than dopamine promoted proliferation neural stem...
Parkinson's disease (PD) is a common neurodegenerative disorder characterized by slow and progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc). Levodopa (l-Dopa), current main treatment for PD, supplies dopamine, but it does not prevent neurodegeneration. There thus no promising remedy PD. Recent vitro study showed increase phosphorylation levels Collapsin Response Mediator Protein 2 (CRMP2) involved axon degeneration. In present study, we report...
Abstract Benzodiazepines are commonly used as sedatives, sleeping aids, and anti‐anxiety drugs. However, chronic treatment with benzodiazepines is known to induce dependence, which considered related neuroplastic changes in the mesolimbic system. This study investigated involvement of K + ‐Cl − co‐transporter 2 (KCC2) sensitization morphine‐induced hyperlocomotion after zolpidem [a selective agonist γ‐aminobutyric acid A‐type receptor (GABA A R) α1 subunit]. In this study, enhanced...
Extremely preterm infants frequently have patent ductus arteriosus (PDA). Recent recommendations include immediately beginning amino acid supplementation in extremely infants. However, the effect of acids on closure (DA) remains unknown.Methods and Results:Aminogram results human neonates at day 2 revealed that plasma glutamate concentration was significantly lower (<28 weeks' gestation) with PDA than those without relatively mature (28-29 weeks gestation). To investigate DA closure,...
l-3,4-dihydroxyphenylalanine (l-DOPA) is a candidate neurotransmitter. l-DOPA released by nicotine through nicotinic receptors. Recently, G-protein coupled receptor GPR143, was identified as for l-DOPA. In this study, genetic association studies between GPR143 polymorphisms and smoking behaviors revealed that the single-nucleotide polymorphism rs6640499, in gene, associated with traits of Japanese individuals. Gpr143 gene-deficient mice, nicotine-induced hypolocomotion rewarding effect were...
Pulmonary hypertension (PH) is a severe and progressive disease that causes elevated right ventricular systolic pressure, hypertrophy ultimately heart failure. However, the underlying pathophysiologic mechanisms are poorly understood. We previously showed 3,4-l-dihydroxylphenyalanine (DOPA) sensitizes vasomotor response to sympathetic tone via coupling between adrenergic receptor alpha1 (ADRA1) G protein-coupled 143 (GPR143), DOPA receptor. investigated whether similarly enhances...
Abstract Nociception is important perception that has harmful influence on daily life of humans. As to main pain management system, some descending pathways are called antinociceptive systems (DAS). DAS, it well known dorsal raphe (B6/B7) - rostral ventromedial medulla (B3) spinal horn includes serotonergic system. However, possible role supralemniscal (B9) serotonin (5-HT) cell group in still open question. In this study, we measured activities B9 5-HT neuronal bodies and neuron-derived...
In the central nervous system, A6 noradrenaline (NA) and B3 serotonin (5-HT) cell groups are well-recognized players in descending antinociceptive while other NA/5-HT not well characterized. A5/A7 NA B2 5-HT cells project to spinal horn form pathways. We recorded G-CaMP6 green fluorescence signal intensities of awake mice response acute tail pinch stimuli, heat context a non-noxious control test, using fiber photometry with calcium imaging system. first introduced or neuronal soma,...
Abstract Most opioid receptor agonists have abuse potential, and the rewarding effects of opioids can be reduced in presence pain. While each enantiomers pentazocine has a differential pharmacologic profile, (±)‐pentazocine been used clinically for treatment However, little information is available regarding which components are associated with its effects, whether (±)‐pentazocine‐induced suppressed under Therefore, present study was performed to investigate pain on acquisition...