A5063259418- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Complement system in diseases
- Cytokine Signaling Pathways and Interactions
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Endoplasmic Reticulum Stress and Disease
- Spaceflight effects on biology
- Peptidase Inhibition and Analysis
- Marine Sponges and Natural Products
- Axon Guidance and Neuronal Signaling
- Muscle metabolism and nutrition
- interferon and immune responses
- Immune Response and Inflammation
- Marine Invertebrate Physiology and Ecology
- Developmental Biology and Gene Regulation
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Mechanisms of cancer metastasis
- Neurogenesis and neuroplasticity mechanisms
- Machine Learning in Bioinformatics
- Coral and Marine Ecosystems Studies
- Sphingolipid Metabolism and Signaling
- Mosquito-borne diseases and control
- TGF-β signaling in diseases
Yokohama City University
2012-2021
Yokohama City University Medical Center
2015-2021
Gumma Paz College
2020-2021
The University of Tokyo
2006-2009
Nagoya University
2009
Cephalochordates, urochordates, and vertebrates evolved from a common ancestor over 520 million years ago. To improve our understanding of chordate evolution the origin vertebrates, we intensively searched for particular genes, gene families, conserved noncoding elements in sequenced genome cephalochordate Branchiostoma floridae, commonly called amphioxus or lancelets. Special attention was given to homeobox opsin genes involved neural crest development, nuclear receptor encoding components...
Interferon regulatory factor-5 (IRF5), a transcription factor critical for the induction of innate immune responses, contributes to pathogenesis autoimmune disease systemic lupus erythematosus (SLE) in humans and mice. Lyn, Src family kinase, is also implicated human SLE, Lyn-deficient mice develop an SLE-like disease. Here, we found that Lyn physically interacted with IRF5 inhibit ubiquitination phosphorylation TLR-MyD88 pathway, thereby suppressing transcriptional activity manner...
The 26S proteasome is a large, complex multisubunit protease involved in protein quality control and other critical processes eukaryotes. More than 110 post-translational modification (PTM) sites have been identified by mass spectrometry of the Saccharomyces cerevisiae are predicted to be implicated dynamic regulation proteasomal functions. Here, we report that N-myristoylation Rpt2 subunit controls intracellular localization proteasome. While proteasomes were mainly localized nucleus normal...
Collapsin response mediator protein 2 (CRMP2) plays a key role in axon guidance, dendritic morphogenesis and cell polarization. CRMP2 is implicated various neurological psychiatric disorders. However, vivo functions of remain unknown. We generated gene-deficient (crmp2-/- ) mice examined their behavioral phenotypes. During 24-h home cage monitoring, the activity level during dark phase crmp2-/- was significantly higher than that wild-type (WT) mice. Moreover, time open arm an elevated plus...
Although there are numerous reports describing the in vivo bone forming capability of recombinant human morphogenetic proteins-2 (rhBMP-2), studies have reported limited effects on mesenchymal stem cells (hMSCs). However, reasons for these discrepancies not well understood. The aim this study was to investigate responsiveness hMSCs osteoinductive signals, focusing rhBMP-2 and effect serum that responsiveness. Human MSCs from six donors were analysed. When those treated with osteoinduction...
Protein phosphorylation is one of the major factors involved in tumor progression and malignancy. We performed exploratory studies aimed at identifying phosphoproteins characteristic to cell lines derived from ovarian clear adenocarcinoma (CCA), a highly malignant type cancer. Comparative phosphoproteome analysis revealed that phosphopeptides five SWI/SNF chromatin remodeling/tumor suppressor components, including ARID1A BRG1, were significantly down-regulated CCA cells. then quantitatively...
Alzheimer's disease (AD) is an incurable neurodegenerative characterized by memory loss and neurotoxic amyloid beta (Aβ) plaques accumulation. Numerous pharmacological interventions targeting Aβ accumulation have failed to alleviate AD. Also, the pathological alterations in AD start years before onset of clinical symptoms. To identify proteins at play during early stage AD, we conducted proteomic analysis hippocampus young AppNL-F mice model preclinical phase disease. This was followed...
Abstract Short-chain fatty acids produced by the gut bacterial fermentation of non-digestible carbohydrates, e.g., fructo-oligosaccharide (FOS), contribute to maintenance skeletal muscle mass and oxidative metabolic capacity. We evaluated effect FOS ingestion on protein expression soleus (Sol) extensor digitorum longus muscles in mice exposed microgravity (μ- g ). Twelve 9-week-old male C57BL/6J were raised individually International Space Station under μ- or artificial 1- fed a diet with...
To identify circulating factors as candidates involved in type 2 diabetes mellitus (T2DM), we conducted two different quantitative proteomic analyses: (1) db/db mouse sera were compared with db/+ obtained at 4, 8, 12, and 24 weeks of age, (2) from animals treated liraglutide without treatment. Twenty proteins differentially expressed the first experiment eight after treatment second experiment. Soluble epidermal growth factor receptor (sEGFR) was identified a common factor, its protein level...
Immunoglobulin A (IgA)-albumin complexes may be associated with pathophysiology of multiple myeloma, although the etiology is not clear. Detailed structural analyses these protein–protein contribute to our understanding this disease. We analyzed structure IgA-albumin complex using various electrophoresis, mass spectrometry, and in silico techniques. The data based on electrophoresis spectrometry showed that IgA sera patients was dimeric, linked via J chain. Only dimeric can bind albumin...
Abstract The transcription factor interferon regulatory 5 (IRF5) is critical for innate immune responses downstream of Toll-like receptors (TLRs). IRF5 also linked to the pathogenesis systemic lupus erythematosus (SLE) in humans and mice. However, it remains elusive whether how activity can be negatively regulated. Src family kinase Lyn implicated human SLE, Lyn−/− mice develop an SLE-like disease. Here we show that selectively inhibits activity, while does not affect NF-κB pathway....