A5000020735- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Salivary Gland Disorders and Functions
- dental development and anomalies
- Dental Implant Techniques and Outcomes
- Virus-based gene therapy research
- Oral and Maxillofacial Pathology
- Bone and Dental Protein Studies
- Dental Trauma and Treatments
- Proteoglycans and glycosaminoglycans research
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Bone Metabolism and Diseases
- Bone Tumor Diagnosis and Treatments
- Pancreatic function and diabetes
- Corneal Surgery and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Dysphagia Assessment and Management
- Cancer Cells and Metastasis
Aichi Medical University
2022-2025
Matsumoto Dental University
2013-2022
The University of Tokyo
2011-2022
University of Tokyo Hospital
2018-2021
Tokyo University of Science
2019
Institute of Medical Sciences
2013
Nagoya University
2002-2011
Tissue Dynamics (Israel)
2011
Tokyo Medical University
2009
Nihon University
2009
To investigate the role of HOX-like homeoprotein PDX1 in formation and maintenance pancreas, we have genetically engineered mice so that only source is a transgene can be controlled by application tetracycline or its analogue doxycycline. In these coding region for tetracycline-regulated transactivator (tTA off ) has replaced endogenous Pdx1 gene to ensure correct temporal spatial expression regulatable transactivator. absence doxycycline, tTA activates transcription bicistronic encoding an...
Novel technologies to establish three-dimensional, in vivo-like tissue consisting of various types cells are required for engineering. We applied magnetic force construct a heterotypic, layered coculture system rat hepatocytes and human aortic endothelial (HAECs) that was not limited by cell type. Magnetite cationic liposomes carrying positive surface charge improve adsorption accumulated HAECs at concentration 38 pg magnetite per cell. Magnetically labeled specifically onto hepatocyte...
Novel technologies for creating three-dimensional constructs with complex shapes would be highly useful in tissue engineering. In the present study, tubular structures were constructed using magnetic force. Magnetite nanoparticles cationic liposomes taken up by target cells. The magnetically labeled cells seeded onto ultralow-attachment plates, and a magnet was placed under wells. After 24 h of culture, formed cell sheet. Subsequently, when cylindrical rolled sheet, sheet attracted to tube...
Bone augmentation via tissue engineering has generated significant interest. We hypothesized that periosteum-derived cells could be used in place of bone marrow stromal (which are widely used) engineering, but the differences osteogenic potential between these 2 cell types unclear. Here, we compared cells, and investigated optimal osteoinductive conditions for cells. Both were induced, bFGF BMP-2, to differentiate into osteoblasts. Periosteal proliferated faster than markers indicated more...
Human bone marrow mesenchymal stem cells (hBMSCs) are widely used cell source for clinical regeneration. Achieving the greatest therapeutic effect is dependent on osteogenic differentiation potential of to be implanted. However, there still no practical methods characterize such non-invasively or previously. Monitoring cellular morphology a and non-invasive approach evaluating potential. Unfortunately, image-based approaches had been historically qualitative requiring experienced...
Novel technologies to establish three-dimensional constructs are desired for tissue engineering. In the present study, magnetic force was used construct multilayered keratinocyte sheets and harvest without enzymatic treatment. Our original magnetite cationic liposomes, which have a positive surface charge in order improve adsorption, were taken up by human keratinocytes at concentration of 33 pg per cell. The magnetically labeled (2×106 cells, corresponds 5 times confluent against culture...
Abstract Objectives : The morbidity and mortality of the dependent elderly that result from aspiration pneumonia are recognized as a major geriatric health problem. Most cases bacterial initiated following colonization or superinfection pharynx by pathogenic bacteria, followed pharyngeal contents. A recent study revealed commonly cause respiratory infection, colonized dentures elderly. This suggests denture plaque may function reservoir potential pathogens to facilitate on pharynx. purpose...
Choroidal neovascularization (CNV) is the most severe form of age-related macular degeneration (AMD), which causes rapid visual loss. Transplantation cultured retinal pigment epithelium (RPE) cell sheet by tissue engineering a possible approach to treatment CNV. In present study, we investigated possibility using magnetite nanoparticles and magnetic force construct deliver RPE sheets in vitro. When cationic liposomes (MCLs), having positive charge at surface, were added ARPE-19 human cells...
Chitosan nanoparticle (nanochitosan) has a broad antimicrobial spectrum against diverse pathogenic microorganisms. However, its effect on dental caries-associated microorganisms, such as Streptococcus mutans and Candida albicans is yet to be explored. These microorganisms are known for causing early childhood caries. Therefore, this study was aimed at investigating nanochitosan inhibition capacity dual-species biofilms of S. C. albicans. In study, activity reported mono dual biofilm species...
Homeostasis is essential for the maintenance of health, and how it maintained focus attention in homeostatic medicine. As a natural dietary nutrient, nitrate critical regulating homeostasis. This article summarizes nitrate's cognitive history, sources, metabolism. It discusses relationship possible mechanism between organism homeostasis from three aspects: flora homeostasis, inflammation-immunity energy metabolism as well clinical application scenarios promising new generation drugs,...
The successful regeneration of complex tooth structures based on tissue-engineering principles was recently reported. process this regeneration, however, remains poorly characterized. In study, we have used histochemistry to examine the tissue engineered teeth in order determine cell types that give rise these structures. Porcine third molar buds were dissociated into single-cell suspensions and seeded onto a biodegradable polyglycolic acid polymer scaffold. Following varying periods growth...
Abstract Recently, decellularized tissue has been reported to have the potential regenerate a variety of tissues. However, optimal protocol for esophagus not studied. Here, we investigated effect different decellularization protocols on histology andbiocompatibility esophagi in view future applications engineering. The esophageal mucosal epithelium (EP) from 4‐week‐old Wistar rats was enzymatically dissociated and cultured with growth‐arrested feeder cells. Two methods usingdeoxycholic acid...
For successful tissue engineering, neovascularization of the implanted is critical. Factors generated by endothelial cells are also considered crucial for process osteogenesis. The direct effects supplementing engineered constructs with cultured progenitor (EPCs) enhancing bone regeneration have not been reported. In this study, we investigated potential EPCs to facilitate in implants and evaluated their influence on regeneration. EPC soluble factors osteogenic differentiation mesenchymal...
Chitosan has been a popular option for tissue engineering, however exhibits limited function bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO2 nanoparticles improve capability. Morphology crystallographic analysis showed that in anatase-type were distributed evenly on the surface of sponges. Degradation test significant effect addition retaining integrity. Biomineralization assay using simulated body fluid apatite...
The characteristics of cultured mucosal cells from the oral mucosa were investigated and compared with those epidermal cells. Total cell counts showed that possessed greater proliferating ability than results 3(4,5-dimethyle-thiazoyl-2-yl)2,5 diphenyltetrazolium bromide assay confirmed this observation also suggested maintained biological activity longer most important morphological in culture their low grade differentiation. Interestingly, enucleation keratinization progressively during...
The aim of this study was to assess the feasibility using gene transfer salivary glands direct systemic delivery therapeutic protiens in vivo. We used a replication-deficient recombinant adenovirus vector (Adα1AT) that encodes human α1-antitrypsin (hα1-AT), which we as marker protein. Adα1AT (5 × 109 pfu) administered by retrograde ductal instillation submandibular male rats. amount hα1-AT found glands, saliva, serum, and other tissues analyzed sensitive enzyme-linked immunosorbent assay...
Purpose: The aim of this study was to clinically evaluate the success implants placed in conjunction with a new material, tissue-engineered bone, and stability regenerated bone after functional loading on long-term basis. Methods: applied 14 cases, which 6 patients were partially or totally edentulous arches scheduled for sinus floor grafting 8 underwent concurrent onlay plasty. Results: This showed that injectable formation induced anatomical site 100% patients. results also indicate it...