A5013095646- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Mesenchymal stem cell research
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- Antimicrobial Peptides and Activities
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Endometrial and Cervical Cancer Treatments
- X-ray Diffraction in Crystallography
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Protein Hydrolysis and Bioactive Peptides
- Machine Learning in Bioinformatics
- Electrospun Nanofibers in Biomedical Applications
- Crystallization and Solubility Studies
- Cannabis and Cannabinoid Research
- Cancer Cells and Metastasis
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Biochemical Analysis and Sensing Techniques
- Image Processing Techniques and Applications
Nagoya University
2016-2025
Tokai National Higher Education and Research System
2022-2025
Children's Hospital of Los Angeles
2021-2025
Nagoya University of Commerce and Business
2022
University of Kansas
2021
University of California, Berkeley
2021
Tokyo Metropolitan Institute of Public Health
2015-2016
Tokyo Medical University
2016
Hosei University
2016
Meijo University
2015
Human bone marrow mesenchymal stem cells (hBMSCs) are widely used cell source for clinical regeneration. Achieving the greatest therapeutic effect is dependent on osteogenic differentiation potential of to be implanted. However, there still no practical methods characterize such non-invasively or previously. Monitoring cellular morphology a and non-invasive approach evaluating potential. Unfortunately, image-based approaches had been historically qualitative requiring experienced...
Peptide uptake systems that involve members of the proton-coupled oligopeptide transporter (POT) family are conserved across all organisms. POT proteins have characteristic substrate multispecificity, with which one can recognize as many 8,400 types di/tripeptides and certain peptide-like drugs. Here we characterize multispecificity Ptr2p, a major peptide Saccharomyces cerevisiae, using dipeptide library. The affinities (Ki) toward Ptr2p show wide distribution range from 48 mM to 0.020 mM....
Given the difficulties inherent in maintaining human pluripotent stem cells (hPSCs) a healthy state, hPSCs should be routinely characterized using several established standard criteria during expansion for research or therapeutic purposes. hPSC colony morphology is typically considered an important criterion, but it not evaluated quantitatively. Thus, we designed unbiased method to evaluate morphology. This involves combination of automated non-labelled live-cell imaging and implementation...
Controlling the balance of endothelial cells (ECs) and smooth muscle (SMCs) in blood vessels is critically important to minimize risk associated with vascular implants. Extracellular matrix (ECM) plays a key role controlling cellular balance, suggesting promising source cell-selective peptides. To obtain EC- or SMC-selective peptides, we start by highlighting sequence differences found among ECM molecules as enriched targets for We explored performance tripeptides that are specifically only...
Precise quantification of cellular potential stem cells, such as human bone marrow-derived mesenchymal cells (hBMSCs), is important for achieving stable and effective outcomes in clinical cell therapy. Here, we report a method image-based prediction the multiple differentiation potentials hBMSCs. This has four major advantages: (1) used are fully intact, therefore directly usable applications; (2) predictions generated before cultures initiated; (3) can be provided simultaneously each...
From the recent advances, there are growing expectations toward mass production of induced pluripotent stem cells (iPSCs) for varieties applications. For such type industrial cell manufacturing, technology which can stabilize efficiency is strongly required. Since present iPSC culture covered by delicate manual operations, still quality differences in produced from same protocols. To monitor process iPSCs with quantified data to evaluate status, we here introduce image-based visualization...
We have developed magnetic cationic liposomes (MCLs) that contained nanoparticles as heating mediator for applying them to local hyperthermia. The performance of the MCLs is significantly affected by property incorporated magnetite nanoparticles. estimated capacity measuring its specific absorption rate (SAR) against irradiation alternating field (AMF).Magnetite which various specific-surface-area (SSA) are dispersed in sample tubes, subjected AMF and studied SAR.Heat generation particles...
Abstract In the development process for drugs used to treat skeletal muscle, cell‐based contractile force assays have been considered as a useful in vitro test. Immortalized human myogenic cells are promising cell sources reproducible and well‐characterized models. this study, it is investigated whether immortalized cells, Hu5/KD3, suitable ability potential be assays. Muscle tissues fabricated using Hu5/KD3 on microfabricated devices measure force. The generate tetanic of ≈30 µN response...
Abstract Transplantation of retinal pigment epithelial (RPE) sheets derived from human induced pluripotent cells (hiPSC) is a promising cell therapy for RPE degeneration, such as in age-related macular degeneration. Current replacement therapies, however, face major challenges. They require tedious manual process selecting differentiated hiPSC-derived cells, and despite wide variation quality sheets, there exists no efficient distinguishing functional those unsuitable transplantation. To...
Adipose tissue-derived stem/progenitor cells (ASCs) have been reported to differentiate not only into mesodermal such as osteoblasts, chondorocytes, and adipocytes, but also endodermal hepatocytes insulin-expressing cells. These are expected be used for variety of regenerative therapies. This study demonstrates the viability adipo/osteogenic potential cryopreserved ASCs using seven cryopreservation solutions, including 10% DMSO, Cell Freezing Medium-DMSO, Medium-Glycerol, Banker 1, 1+, 2,...
Abstract We developed a method to improve protein thermostability, “loop-walking method”. Three consecutive positions in 12 loops of Burkholderia cepacia lipase were subjected random mutagenesis make libraries. Screening allowed us identify L7 as hot-spot loop having an impact on and the P233G/L234E/V235M mutant was found from 214 variants library. Although more excellent might be discovered by screening all 8000 P233X/L234X/V235X mutants, it difficult assay them. therefore employed machine...
Abstract This work presents an image‐based hybrid model incorporating the initial spatial distribution for mesenchymal stem cell (MSC) cultivation process design. First, three levels of seeding bias in static MSC were experimentally investigated, and resulting was quantified using phase contrast microscopy image analysis. Second, observed heterogeneity defined as a new parameter by calculating standard deviation seeded number fraction among numerical 8 × tiles on two‐dimensional plates....