A5015305494- Spaceflight effects on biology
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Muscle activation and electromyography studies
- High Altitude and Hypoxia
- Sports Performance and Training
- Cardiovascular and exercise physiology
- Heat shock proteins research
- Diet and metabolism studies
- Biochemical effects in animals
- Thermoregulation and physiological responses
- Mitochondrial Function and Pathology
- Cardiomyopathy and Myosin Studies
- Iron Metabolism and Disorders
- Children's Physical and Motor Development
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Neurodegenerative Diseases
- Transcranial Magnetic Stimulation Studies
- Cancer, Hypoxia, and Metabolism
- Sports injuries and prevention
- Bone health and osteoporosis research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Prosthetics and Rehabilitation Robotics
Doshisha University
2014-2022
Osaka University of Health and Sport Sciences
2005-2022
National Center of Space Research and Technology
2020
Osaka University
2007-2019
Development Initiatives
2019
Tottori University
2018
University of Genoa
2016
Faculty (United Kingdom)
2015
Graduate School USA
2006-2015
National Institute of Fitness and Sports in Kanoya
1991-2015
The effects of 14 days spaceflight on myonuclear number, fiber size, and myosin heavy chain (MHC) expression in isolated rat soleus muscle segments were studied. Single fibers from rats flown the Spacelab Life Sciences-2 14-day mission compared with those age-matched ground-based control by using confocal microscopy gel electrophoresis. Spaceflight resulted a significant reduction number expressing type I MHC an increase IIx or IIa MHC. Space-flight also percentage coexpressing more than one...
The effect of microgravity on skeletal muscles has so far been examined in rat and mice only after short-term (5–20 day) spaceflights. drawer system (MDS) program, sponsored by Italian Space Agency, for the first time aimed to investigate consequences long-term (91 days) exposure within International Station. Muscle atrophy was present indistinctly all fiber types slow-twitch soleus muscle, but slightly greater than that observed 20 days spaceflight. Myosin heavy chain analysis indicated a...
After 2 or 4 mo of bed rest (6 degrees head-down tilt) and 1 ambulation, there was a tendency toward higher percentage fibers expressing fast myosin heavy chain (MHC) isoforms de novo appearance coexpressing type I+IIa+IIx IIa+IIx MHC in human soleus fibers. rest, the mean size I decreased by 12 (P > 0.05) 39%, respectively. Because myonuclear number/mm fiber length unchanged, domain smaller after than before. The were largest recovery. effects wearing an antigravity device (Penguin suit),...
S ummary . Changes in haemoglobin (Hb), work performance, heart rate and postexercise blood lactate were studied iron deficient, anaemic subjects for 16 d following total dosage infusion of dextran, i.v. (30–50 ml). Six adult men 14 women with initial Hb levels 6.6±0.6 g/dl (mean±SEM) the treatment group (n=10) 8.0±0.7 placebo (saline infusion, n=10). Serum 0.51±0.15 0.67±0.12 mg/l two groups, respectively. Haemoglobin maximal time increased significantly within 4 after continued to increase...
Lactate is produced in and released from skeletal muscle cells. receptor, G-protein-coupled receptor 81 (GPR81), expressed However, a physiological role of extracellular lactate on not fully clarified. The purpose this study was to investigate lactate-associated morphological changes intracellular signals C2C12 cells.Mouse myoblast cells were differentiated for 5 days form myotubes. Sodium (lactate) or GPR81 agonist, 3,5-dihydroxybenzoic acid (3,5-DHBA), administered the differentiation...
The effects of mechanical unloading and reloading on the properties rat soleus muscle fibers were investigated in male Wistar Hannover rats. Satellite cells control rats distributed evenly throughout fiber length. After 16 days hindlimb unloading, number satellite central, but not proximal or distal, region was decreased. central gradually increased during 16-day period reloading. mean sarcomere length passively shortened due to plantarflexed position at ankle joint: maintained <2.1 microm,...
Effects of 3-month exposure to microgravity environment on the expression genes and proteins in mouse brain were studied. Moreover, responses neurobiological parameters, nerve growth factor (NGF) derived neurotrophic (BDNF), also evaluated cerebellum, hippocampus, cortex, adrenal glands. Spaceflight-related changes gene protein observed. Biological processes up-regulated related immune response, metabolic process, and/or inflammatory response. Changes cellular components involving microsome...
It is now evident that many nuclear hormone receptors can modulate target gene expression. REV-ERBα, one of the with capacity to alter clock function, critically involved in lipid metabolism, adipogenesis, and inflammatory response. Recent studies suggest REV-ERBα plays a key role mediation between clockwork inflammation. The purpose current study was investigate regulation interleukin-6 (il6) expression murine macrophages. agonists, or overexpression rev-erb α macrophage cell line RAW264...
An original method to induce heat stress was used clarify the time course of changes in shock proteins (HSPs) rat skeletal muscles during recovery after a single bout stress. One hindlimb inserted into stainless steel can and directly heated by raising air temperature inside via flexible heater twisted around can. Muscle increased gradually maintained at 42°C for 60 min. Core rectal contralateral muscle temperatures were <1.5°C HSP60, HSP72, cognate (HSC) 73 content slow soleus fast...
The effects of heat-stress on proliferative potential in vivo were studied rat skeletal muscle. Male Wistar rats (7-weeks-old) divided into two groups: control (n = 24) and heat-stressed 24). Rats the experimental group exposed to environmental (41°C for 60 min) a heat chamber without anesthesia. soleus muscles dissected 1, 7, 14 days after exposure. wet dry weights muscle relative body weight significantly higher than controls 7 exposure (10.1% 17.5%, respectively, p < 0.05)....
Responses of electromyogram (EMG) in soleus muscle and both afferent efferent neurograms at the fifth lumbar (L(5)) segmental level spinal cord were investigated during acute chronic unloading induced by hindlimb suspension and/or tenotomy adult rats. The EMG neurogram decreased 88 37%, respectively, relative to those quadrupedal posture on floor after that causes passive shortening due ankle plantarflexion. However, (P < 0.05) > recorded increased synergists. Furthermore, input was...
Selected parameters related to work tolerance were measured in 31 adult subjects with hemoglobin (Hb) concentration from 2.5 14.0g/100ml. Work was closely Hb (r=0.74) regardless of the adequacy storage iron level. One male and six females a mean 3.5g/100ml (27-55 years old) studied before 24hr after transfusing 570ml whole blood. The maximal load tolerated increased 83% within transfusion these seven subjects. Post-exercise venous blood lactate markedly lower transfusion. same as other who...
AMPK is considered to have a role in regulating skeletal muscle mass. However, there are no studies investigating the function of modulating mass during atrophic conditions. In present study, we investigated difference unloading-associated atrophy and molecular functions response 2-wk hindlimb suspension between transgenic mice overexpressing dominant-negative mutant (AMPK-DN) their wild-type (WT) littermates. Male WT (n = 24) AMPK-DN were randomly divided into two groups: an untreated...
The effects of icing or heat stress on the regeneration injured soleus muscle were investigated in male Wistar rats. Bupivacaine was injected into muscles bilaterally to induce injury. Icing (0 °C, 20 min) carried out immediately after Heat (42 30 applied every other day during 2-14 days bupivacaine injection. Injury-related increase collagen deposition promoted by icing. However, level heat-stressed animals maintained at control levels throughout experimental period and significantly lower...
The effects of 2 and 4 mo bed rest, with or without exercise countermeasures, on the contractile properties slow fibers in human soleus muscle were examined. Mean fiber diameters 8 36% smaller after respectively, than pre-bed rest level. Maximum tetanic force (P(o)), maximum activated (F(max)) per cross-sectional area (CSA), common-logarithm value free Ca(2+) concentration required for half-maximal activation (pCa(50)) also decreased rest. In contrast, unloaded shortening velocity (V(o)) was...
Effects of heat stress on the recovery atrophied soleus muscle were studied in rats. Ten-week-old male Wistar rats randomly divided into cage control (CC) and 5-day hindlimb suspension group (HS). The half HS was exposed to (41°C for 60 min) an incubator immediately after (HS-H) other not stressed (HS-C) prior 10 days ambulation recovery. One (CH) also similarly. muscles dissected at four time points, i.e., (or stress), 3, 5, (n = 8 per each point). absolute wet weight water protein...
Effects of gravitational loading or unloading on the growth-associated increase in cross-sectional area and length fibers, as well total fiber number, soleus muscle were studied rats. Furthermore, roles satellite cells myonuclei growth these properties also investigated. The hindlimb by tail suspension was performed newborn rats from postnatal day 4 to month 3 with without 3-mo reloading. morphological measured whole and/or single fibers sampled tendon tendon. Growth-associated increases...
Effects of 14 days hindlimb unloading or synergist ablation-related overloading with without deafferentation on the fiber cross-sectional area, myonuclear number, size, and domain, number nucleoli in a single myonucleus, levels phosphorylation ribosomal protein S6 (S6) 27-kDa heat shock (HSP27) were studied rat soleus. Hypertrophy fibers (+24%), associated increased nucleolar (from 1-2 to 3-5) within myonucleus domain (+27%) compared preexperimental level, was induced by ablation. Such...
The relative importance of neural and mechanical influences in maintaining normal slow fast muscle properties remains unclear. To address this issue, we studied the effects 10 days hindlimb unloading (HU) with or without tenotomy and/or denervation on cross-sectional area (CSA), myosin heavy chain (MHC) expression (immunohistochemistry) composition (gel electrophoresis), myonuclear number soleus plantaris fibers adult male Wistar rats. In general, adaptations fiber type size were similar...