A5008260162- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Heart Failure Treatment and Management
- Pain Mechanisms and Treatments
- Hypothalamic control of reproductive hormones
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Pharmacogenetics and Drug Metabolism
- Hormonal Regulation and Hypertension
- Inflammatory mediators and NSAID effects
- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Stress Responses and Cortisol
- Atrial Fibrillation Management and Outcomes
- Peptidase Inhibition and Analysis
- Drug Transport and Resistance Mechanisms
- Steroid Chemistry and Biochemistry
- Neuroscience and Neuropharmacology Research
- Estrogen and related hormone effects
- Protein Hydrolysis and Bioactive Peptides
- Mitochondrial Function and Pathology
- Pharmacology and Obesity Treatment
- Amino Acid Enzymes and Metabolism
- Cardiac Arrhythmias and Treatments
- Microbial metabolism and enzyme function
- Electrolyte and hormonal disorders
Kagoshima University
2014-2024
University of Toyama
2018
Showa University
2018
Kagoshima University Hospital
2006
National Cerebral and Cardiovascular Center
1992-2002
Tulane University
1989-2000
Miyazaki Welfare Medical College
1987-1997
Kinki Central Hospital
1997
Max Planck Institute of Biochemistry
1994
University of Konstanz
1994
The human gene ( PTGS2 ) encoding an inducible isozyme of prostaglandin‐endoperoxide synthase (prostaglandin‐endoperoxide 2) that is distinct from the well‐characterized and constitutive 1), was isolated using a polymerase‐chain reaction‐generated cDNA fragment probe for 2. Nucleotide sequence analysis entire prostaglandin‐endoperoxide‐synthase‐2 demonstrated it more than 8.3 kb in size consists ten exons; this very similar to murine chicken genes. structures exons were also those...
A novel bioactive peptide was recently isolated from ovine hypothalamus and named PACAP (pituitary adenylate cyclase-activating polypeptide). present in two bioactive, amidated forms, PACAP27 PACAP38 (27 38 amino acids, respectively), showed a 68% sequence homology with vasoactive intestinal (VIP) the Nterminal 28 residues. at least 1000 times more potent than VIP stimulating cyclase pituitary cells, but both peptides exhibited comparable vasodepressor activity. Thus, we sought to determine...
alpha-Tocopherol transfer protein (alpha TTP), which specifically binds this vitamin and enhances its between separate membranes, was previously isolated from rat liver cytosol. In the current study we demonstrated presence of alpha TTP in human by isolating cDNA a library. The for predicts 278 amino acids with calculated molecular mass 31,749, sequence exhibits 94% similarity at acid level. recombinant expressed Escherichia coli both alpha-tocopherol activity an vitro assay cross-reactivity...
alpha-Tocopherol transfer protein (alpha TTP) present in rat liver cytosol specifically binds this vitamin and enhances its between separate membranes. We previously reported purification of alpha TTP showed that two isoforms exist liver, which the isoelectric points are 5.0 5.1, respectively (Sato, Y., Hagiwara, K., Arai, H., Inoue, K. (1991) FEBS Lett. 288, 41-45). In paper, we have isolated a cDNA clone with 2194 base pairs encoding from library. The amino acid sequence deduced contained...
In the present study we investigated form of expression, action, second messenger, and cellular location urocortin, a member corticotropin-releasing factor (CRF) family, in heart. Urocortin mRNA, as shown by quantitative RT-PCR analysis, is expressed cultured rat cardiac nonmyocytes (NMC) well myocytes (MC) heart, whereas CRF receptor type 2β (CRF-R2β), presumed urocortin predominantly MC compared with NMC. mRNA expression higher left ventricular (LV) hypertrophy than normal LV, CRF-R2β...
Prostacyclin synthase catalyzes the conversion of prostaglandin H, to prostacyclin, which is a powerful vasodilator and most potent natural occurring inhibitor platelet aggregation.In present study, we determined amino acid sequence bovine prostacyclin by combined protein chemical molecular cloning techniques.The enzyme was purified characterized from aorta microsomes, partial sequences were with native endoproteinase Lys-C-cleaved peptides.Using primers synthesized according sequences, cDNA...
We characterized highly selective receptors for PACAP, the pituitary adenylate cyclase activating peptide, in tumoral acinar cell line AR 4‐2J derived from rat pancreas. a novel hypothalamic peptide related to vasoactive intestinal (VIP), was tested as full natural 38‐residue (PACAP‐38) and an N‐terminal amidated 27‐residue derivative (PACAP‐27). The binding sites showed considerable affinity [ 125 I]PACAP‐27 ( K d =0.4 nM) PACAP‐38, while their affiity VIP parent helodemin 1000‐fold lower....
Human cDNAs encoding the precursor to pituitary adenylate cyclase activating polypeptide (PACAP) were cloned from human testis and cerebral cortex cDNA libraries. Nucleotide sequencing revealed that library encoded entire for PACAP, while brain represented only carboxy-terminal half of precursor. The predicted PACAP consisted 176 amino acid residues was very similar ovine one (82%). Both precursors contained both another peptide, PACAP-related peptide (PRP), having 29 acids. PRP preceded...
In skeletal muscle, excitation-contraction (E-C) coupling requires the conversion of depolarization signal invaginated surface membrane, namely transverse (T-) tubule, to Ca2+ release from sarcoplasmic reticulum (SR). Signal transduction occurs at junctional complex between T-tubule and SR, designated as triad junction, which contains two components essential for E-C coupling, dihydropyridine receptor T-tubular voltage sensor ryanodine SR Ca2+-release channel. However, functional expression...
Plasma adrenomedullin (AM) levels are reportedly increased in heart failure, but whether the cardiac production and secretion of AM is failure remains unknown. To investigate sites we measured plasma peptide mRNA various tissues rats with failure. We also examined actually secretes into circulation patients tissue by specific radioimmunoassay Northern blot analysis produced aortocaval fistula. coronary sinus aorta left ventricular dysfunction before after rapid right pacing. The increase...
Adrenomedullin, a potent hypotensive peptide, reduces blood pressure and pulmonary vascular resistance, increases flow. The mRNA for adrenomedullin its receptor is highly expressed in the lung, suggesting regulatory role circulation. To investigate clinical significance of patients with hypertension, we studied relationship between plasma levels haemodynamics. Venous, arterial samples were obtained during cardiac catheterization measured by specific radioimmunoassay 33 consecutive severe...
<h3>Objectives</h3> To investigate the pathophysiological role of adrenomedullin in myocardial infarction. <h3>Patients and design</h3> Plasma concentrations adrenomedullin, atrial natriuretic factor, brain peptide were measured by radioimmunoassay 31 patients with acute infarction over four weeks, 44 normal subjects. <h3>Results</h3> In infarction, plasma reached a peak (mean (SD) 14.0 (9.0) pmol/l at 24 hours after onset symptoms remained increased all sampling points except week point...
The comet assay has been widely used as a genotoxicity test for detecting primary DNA damage in individual cells. micronucleus (MN) is also well-established clastogenicity and aneugenicity. A combination of the (COM) MN capable variety genotoxic potentials an vitro screening system. Although robust protocol Organisation Economic Co-operation Development (OECD) guideline, COM does not. To establish to compare its sensitivity with that MN, we conducted concurrently five agents (ethyl...
C-type natriuretic peptide (CNP) is abundant in brain and reported to exert autocrine function vascular cells, but its effect on blood–brain barrier (BBB) permeability has not been clarified yet. Here, we examined this effect. Transendothelial electrical resistance (TEER) of vitro BBB model, composed bovine microvascular endothelial cells astrocytes, was significantly dose dependently decreased by CNP (1, 10, 100 nmol/L). treatment reduced both the messenger RNA (mRNA) protein expressions...
Background The G-protein-coupled receptor 40 (GPR40) is suggested to function as a transmembrane for medium- long-chain free fatty acids and implicated play role in acids-mediated enhancement of glucose-stimulated insulin secretion from pancreas. However, the functional GPR40 nervous system including somatosensory pain signaling has not been fully examined yet. Results Intrathecal injection agonist (MEDICA16 or GW9508) dose-dependently reduced ipsilateral mechanical allodynia CFA SNL models...