A5016165244- Bacterial Infections and Vaccines
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Neonatal and Maternal Infections
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Barrier Structure and Function Studies
- Streptococcal Infections and Treatments
- Cell Adhesion Molecules Research
- Antibiotics Pharmacokinetics and Efficacy
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- HIV Research and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Fungal Infections and Studies
- Legionella and Acanthamoeba research
- Infective Endocarditis Diagnosis and Management
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Antifungal resistance and susceptibility
- Drug Transport and Resistance Mechanisms
- Essential Oils and Antimicrobial Activity
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Respiratory Health Research
Inha University
2012-2024
Hanyang University
1985-2024
Johns Hopkins Medicine
2013-2022
Johns Hopkins University
2013-2022
Rural Development Administration
2000-2018
National Institute of Animal Science
2018
Jeju National University Hospital
2008-2017
University of Baltimore
2015
Johns Hopkins Children's Center
2013
Jeju National University
2011
Objective. The frequency of children who are hospitalized with pneumococcal pneumonia complicated by necrosis, empyema/complicated parapneumonic effusion, and lung abscess seems to be increasing. factors that contribute this increase unclear; therefore, the objective study was describe compare relative frequency, clinical characteristics, outcome those uncomplicated caused Streptococcus pneumoniae in era antibiotic resistance. Methods. A multicenter, retrospective 8 children’s hospitals...
The pulmonary collectins, surfactant proteins A (SP-A) and D (SP-D), have been reported to bind lipopolysaccharide (LPS), opsonize microorganisms, enhance the clearance of lung pathogens. In this study, we examined effect SP-A SP-D on growth viability Gram-negative bacteria. Escherichia coli K12 was reduced in SP-A–null mice increased SP-D–overexpressing mice, compared with strain-matched wild-type controls. Purified inhibited bacterial synthetic functions several, but not all, strains E....
ABSTRACT Extraintestinal Escherichia coli (ExPEC), a heterogeneous group of pathogens, encompasses avian, neonatal meningitis, and uropathogenic E. strains. While several virulence factors are associated with ExPEC, there is no core set that can be used to definitively differentiate these pathotypes. Here we describe multiplex four factor-encoding genes, yfcV , vat fyuA chuA highly strains distinguish three groups : diarrheagenic animal-associated strains, human commensal avian pathogenic...
Outer membrane vesicles (OMVs) are important tools in bacterial virulence but their role the pathogenesis of infections caused by enterohemorrhagic Escherichia coli (EHEC) O157, leading cause life-threatening hemolytic uremic syndrome, is poorly understood. Using proteomics, electron and confocal laser scanning microscopy, immunoblotting, bioassays, we investigated OMVs secreted EHEC O157 clinical isolates for factors cargoes, interactions with pathogenetically relevant human cells,...
In determining the mechanism of neutrophil elastase (NE)–mediated killing Escherichia coli , we found that NE degraded outer membrane protein A (OmpA), localized on surface Gram-negative bacteria. killed wild-type, but not OmpA-deficient, E. . Also, whereas NE-deficient mice had impaired survival in response to sepsis, as compared wild-type mice, presence or absence no influence sepsis been induced with OmpA-deficient These findings define a nonoxidative bacterial by and point OmpA target...
To evaluate the antibiotic susceptibility of Streptococcus pneumoniae isolates obtained from blood and cerebrospinal fluid children with meningitis. describe compare clinical microbiological characteristics, treatment, outcome meningitis caused by S based on antimicrobial administration dexamethasone.Children pneumococcal were identified among a group patients systemic infections who enrolled prospectively in United States Pediatric Multicenter Pneumococcal Surveillance Study at eight...
Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination inhaled Cryptococcus neoformans from the lung to brain. The mechanism(s) by which C. crosses blood-brain barrier (BBB) is key unresolved issue in cryptococcosis. We used both an vivo mouse model and vitro human BBB investigate cryptococcal association with traversal BBB. Exposure brain microvascular endothelial cells (HBMEC) triggered formation microvillus-like membrane protrusions within 15 30 min. Yeast...
ABSTRACT Invasion of endothelial tissues may be crucial in a Listeria monocytogenes infection leading to meningitis and/or encephalitis. Internalization L. into cells has been previously demonstrated by using human umbilical vein as model system. However, during the crossing blood-brain barrier, most likely encounters brain microvascular which are strikingly different from macrovascular or cells. In present study (HBMEC) were used interaction with cells, closely resemble native brain. We...
Infection by the human opportunistic fungal pathogen Candida albicans has been increasing over recent years. In an attempt to understand molecular mechanism of invasion across host tissues, relationship C. enolase plasminogen/plasmin was investigated. is a cell-surface protein and immunodominant antigen in infected patients' sera. Plasminogen abundant plasma protein. Several lines evidence support binding plasminogen. Firstly, it found that various strains were able bind plasminogen its...
Infectious meningitis and encephalitis is caused by invasion of circulating pathogens into the brain. It unknown how dynamically interact with brain endothelium under shear stress, leading to Here, using intravital microscopy, we have shown that Cryptococcus neoformans, a yeast pathogen causes meningoencephalitis, stops suddenly in mouse capillaries similar or smaller diameter than organism, same manner kinetics as polystyrene microspheres, without rolling tethering endothelial surface....
The safety and efficacy of imipenem/cilastatin were evaluated in 21 children, ages 3 to 48 months, with bacterial meningitis. Eradication bacteria from the cerebrospinal fluid was demonstrated within 24 hours antibiotic therapy all but 2 patients who had Haemophilus influenzae type b meningitis ultimately achieved bacteriologic cure after days therapy. Cerebrospinal penetrations imipenem cilastatin determined at various times drug administration mean fluid: serum ratios 14 10% for...
Enterohemorrhagic Escherichia coli (EHEC) strains cause diarrhea and hemolytic uremic syndrome resulting from toxin-mediated microvascular endothelial injury. EHEC hemolysin (EHEC-Hly), a member of the RTX (repeats-in-toxin) family, is an virulence factor increasingly recognized importance. The toxin exists as free EHEC-Hly associated with outer membrane vesicles (OMVs) released by during growth. Whereas lytic towards human endothelium, biological effects OMV-associated on intestinal...
Type VI secretion systems (T6SSs) are involved in the pathogenicity of several Gram-negative bacteria. Based on sequence analysis, we found that a cluster E scherichia coli v irulence f actors (EVF) encoding putative T6SS exists genome meningitis-causing E. K1 strain RS218. The T6SS-associated deletion mutants exhibited significant defects binding to and invasion human brain microvascular endothelial cells (HBMEC) compared with parent strain. Hcp family proteins (the hallmark T6SS),...
A three-dimensional cell culture model of syncytiotrophoblasts recapitulates their secretory, morphological, and microbial resistance phenotypes.
The fungal pathogen Cryptococcus neoformans has a predilection for the central nervous system (CNS), resulting in devastating meningoencephalitis. At present, it is unclear how C. traverses blood–brain barrier (BBB) and causes CNS infection. present study examined characterized interaction of with human brain microvascular endothelial cells (HBMEC), which constitute BBB. Adhesion transcytosis HBMEC by was inoculum- time-dependent occurred both encapsulated acapsulated strains. induced marked...
Objective. To track antibiotic susceptibility of Streptococcus pneumoniae isolates obtained from children with systemic infections and determine outcome treatment. Design. A 3-year (September 1993 through August 1996) prospective surveillance study all invasive pneumococcal in children. Patients. Infants cared for at eight children's hospitals the United States culture-proven infection. Results. One thousand two hundred ninety-one episodes infection were identified 1255 An underlying illness...