A5101806312- Tuberculosis Research and Epidemiology
- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Tryptophan and brain disorders
- Postharvest Quality and Shelf Life Management
- Traditional Chinese Medicine Analysis
- Plant Physiology and Cultivation Studies
- Pharmacological Effects of Natural Compounds
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Mycobacterium research and diagnosis
- Polysaccharides and Plant Cell Walls
- Ginseng Biological Effects and Applications
- Respiratory viral infections research
- Alcohol Consumption and Health Effects
- Functional Brain Connectivity Studies
- Liver Disease and Transplantation
- Shoulder Injury and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer-related molecular mechanisms research
- High Altitude and Hypoxia
- Chromatography in Natural Products
- Berberine and alkaloids research
- Drug-Induced Hepatotoxicity and Protection
Guangxi Medicinal Botanical Garden
2021-2024
Shenzhen Third People’s Hospital
2019-2024
Southern University of Science and Technology
2019-2024
National Clinical Research
2024
Southwest Hospital
2020-2023
Army Medical University
2020-2023
Amos Enterprise (China)
2020
Southwest Medical University
2018
PLA Navy General Hospital
2010-2013
South China Agricultural University
2008-2009
Coinfection of human immunodeficiency virus type 1 (HIV-1) is the most significant risk factor for tuberculosis (TB). The immune responses lung are essential to restrict growth Mycobacterium and avoid emergence disease. Nevertheless, there still limited knowledge about local response in people with HIV-1-TB coinfection.
Yuquan capsule (YQC) is a well-known proprietary Chinese medicine used for the treatment of type 2 diabetes mellitus. The aim this study was to investigate potential mechanism and efficacy YQC in T2DM by means metabolomics. Thirty-two male SD rats were randomly divided into four groups control, diabetic mellitus (T2DM), metformin (Met), YQC. Establishment model high-fat diet (HFD) streptozotocin (STZ). Fasting blood glucose (FBG) weight measured weekly, urine output collected recorded....
The increasing prevalence of multidrug-resistant tuberculosis (MDR-TB) highlights the urgent need for an efficient approach to identify Mycobacterium complex (MTBC) strains resistant rifampicin (RIF) and isoniazid (INH). In response, we developed a CRISPR-Cas14a MTB RIF/INH platform that can detect most common mutations associated with RIF INH resistance. To evaluate sensitivity specificity our platform, carried out comprehensive assessment using clinical isolates M. sputum samples from TB...
Jigucao capsule is a well-known Chinese patent medicine for the treatment of acute and chronic hepatitis cholecystitis. The chemical components were not clear resulting from paucity relevant studies, which hindered research pharmacological mechanism, comprehensive development, utilization in clinical studies. By establishing high-throughput ultra-performance liquid chromatography quadrupole time flight mass spectrometry combination with intelligent UNIFI software data processing platform to...
Drug-resistant tuberculosis (TB) poses a major threat to global TB control; consequently, there is an urgent need develop novel anti-TB drugs or strategies. Host-directed therapy (HDT) emerging as effective treatment strategy, especially for drug-resistant TB. This study evaluated the effects of berbamine (BBM), bisbenzylisoquinoline alkaloid, on mycobacterial growth in macrophages. BBM inhibited intracellular Mycobacterium (Mtb) by promoting autophagy and silencing ATG5, partially...
The host immune system plays a pivotal role in the containment of Mycobacterium tuberculosis (Mtb) infection, and host-directed therapy (HDT) is emerging as an effective strategy to treat (TB), especially drug-resistant TB. Previous studies revealed that expression sirtuin 7 (SIRT7), nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, was downregulated macrophages after Mycobacterial infection. Inhibition SIRT7 with pan-sirtuin family inhibitor (NAM), or by silencing expression,...
Certain circulating cell subsets are involved in immune dysregulation human immunodeficiency virus type 1 (HIV-1) and tuberculosis (TB) co-infection; however, the characteristics role of these subclusters unknown. Peripheral blood mononuclear cells (PBMCs) patients with HIV-1 infection alone (HIV-pre) those HIV-1-TB co-infection without anti-TB treatment (HIV-pre & TB-pre) for 2 weeks TB-pos) were subjected to single-cell RNA sequencing (scRNA-seq) characterize transcriptome different...
Introduction. The acromion is a small section of the scapula which extends anteriorly from spine and acromial angle (AA) prominent bony point at junction lateral border shoulder blade. As well known, morphology are important as their anatomical variation may contribute to pathologies. However, few people have studied association between angle. study explores in association, providing an basis for clinical diagnosis treatment. Material Methods. A total 292 dry, intact scapulae (152 right, 140...
Jigucao capsules (JGCC) have the effects of soothing liver and gallbladder clearing heat detoxification. It is a good medicine for treating acute chronic hepatitis cholecystitis with damp gallbladder. However, existing quality standard JGCC does not content determination items, which conducive to control. In this study, serum pharmacochemistry technology UNIFI data processing software were used identify blood prototype components metabolites under condition obvious drug JGCC, referenced...
infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of are different. Therefore, rapid accurate identification
Fresh fruit of longan ( Dimocarpus Lour.) are susceptible to pericarp browning and aril breakdown. Aril breakdown in is regarded as one the most important factors reducing quality shortening storage life fruit. To better understand molecular mechanism breakdown, expression patterns three expansin EXP ) xyloglucan endotransglucosylase XET genes relation stored at room temperature (25 °C) or low (4 were investigated. The results showed that index increased progressively during 25 4 °C....
Dampness-heat Jaundice Syndrome (DHJS) is a complex Chinese medicine syndrome, while Jigucao capsule (JGCC) an effective compound preparation of for the treatment DHJS about liver and gallbladder, but its mechanism not clear yet. The purpose this study to clarify pathogenesis JGCC. We used ultra-high performance liquid chromatography/mass spectrometry (UPLC/MS) combined with pattern recognition, accompanied advanced software online database urine metabolomics rats. potential biomarkers...
Abstract Background Tuberculosis (TB) caused by Mycobacterium tuberculosis ( M. tb ) remains a global health issue. The characterized virulent H37Rv, avirulent H37Ra and BCG strains are widely used as reference to investigate the mechanism of TB pathogenicity. Here, we attempted determine metabolomic signatures associated with Mycobacterial virulence in human macrophages through comparison metabolite profile THP-1-derived following exposure strains. Results Our findings revealed remarkably...
Abstract BACKGROUND: The aim of the present study was to investigate expression profiles three endo‐1,4‐β‐glucanase (EGase, EC 3.2.1.4) genes during aril breakdown longan fruit stored at room temperature (25 °C), low (10 °C) or on transferring 10 °C for 20 days 25 °C. RESULTS: Three full‐length cDNAs, designated Dl‐EGase1, Dl‐EGase2 and Dl‐EGase3 , were isolated characterized. EGase activity in tissues increased with appearance symptoms, while RNA gel blot analysis revealed that...
Abrus mollis Hance is a traditional Chinese medicine that widely used to treat acute and chronic hepatitis, steatosis, fibrosis. Its therapeutic qualities of it have long been acknowledged, although the active ingredients responsible for its efficacy mechanisms action are unknown. In this study, chemical constituents absorbed into blood from were assessed by using liquid chromatography-quadrupole-time-of-flight mass spectrometry data was analyzed with UNIFI screening platform. The results...
The Yuquan capsules is a commonly used traditional Chinese Patent Medicine for the treatment of diabetes mellitus. In this study, high-throughput analytical method identifying chemical composition was established first time by using ultra-performance liquid chromatography-quadrupole flight mass spectrometry. data obtained were subjected to fragment analysis and combined with UNIFI processing natural products. One-hundred sixteen compounds characterized from capsules. Twelve bioactive...
The extremely slow growth rate of Mycobacterium tuberculosis (Mtb) challenges traditional methods for (TB) diagnosis. Here, we assessed the efficacy a previously developed Mtb antigen-specific gamma interferon (IFN-γ) enzyme-linked immunospot (ELISPOT) performed on bronchoalveolar lavage fluid (BALF) from cohort 414 patients including 333 PTB (202/333 were sputum culture positive) diagnosis PTB. We could confirm that IFN-γ-producing CD4+ T cells concentrated significantly in BALF mononuclear...
SummaryA full-length phenylalanine ammonia-lyase gene (PAL) cDNA, named DlPAL1, was isolated and characterised from harvested longan fruit. The expression pattern of DlPAL1 PAL activities in relation to aril breakdown fruit stored at different temperatures were investigated. results showed that both activity the accumulation mRNA increased during storage 25ºC 10ºC, which coincided with an increase index (ABI). or ABI 4ºC for 40 d, then transferred 2 d. No throughout could be detected,...
Abstract Previous studies demonstrated that transforming growth factor (TGT) β1 plays an immunosuppressive role in clinical tuberculosis. However, the contribution of TGF-β1 gene polymorphisms to human tuberculosis susceptibility remains undetermined. In this study, we showed single-nucleotide (SNPs) were associated with increased discovery cohort (1533 case patients and 1445 controls) validation (832 1084 controls), 2 SNPs located promoter region (rs2317130 rs4803457) are strong linkage...