A5038290557- Biosensors and Analytical Detection
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Traditional Chinese Medicine Studies
- Epilepsy research and treatment
- Anesthesia and Neurotoxicity Research
- Advanced Biosensing Techniques and Applications
- Esophageal Cancer Research and Treatment
- Anesthesia and Pain Management
- Genetics and Neurodevelopmental Disorders
- Glioma Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Traditional Chinese Medicine Analysis
- Digestive system and related health
- Herpesvirus Infections and Treatments
- Tuberous Sclerosis Complex Research
- Clinical Laboratory Practices and Quality Control
- Cardiac electrophysiology and arrhythmias
- Anesthesia and Sedative Agents
- Healthcare and Venom Research
- Cardiomyopathy and Myosin Studies
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Acute Ischemic Stroke Management
Nanjing Medical University
2011-2025
Kangda College of Nanjing Medical University
2025
Guangdong 999 Brain Hospital
2010-2025
Kunming Medical University
2011-2024
Beijing Children’s Hospital
2019-2024
Capital Medical University
2016-2024
Shanxi University
2022-2024
Beijing Chest Hospital
2024
Shenyang Pharmaceutical University
2024
Fujian University of Traditional Chinese Medicine
2024
Background —Mutations in the gene G4.5 result a wide spectrum of severe infantile cardiomyopathic phenotypes, including isolated left ventricular noncompaction (LVNC), as well Barth syndrome (BTHS) with dilated cardiomyopathy (DCM). The purpose this study was to investigate patients LVNC or BTHS for mutations other novel genes. Methods and Results —DNA from 2 families 3 individuals congenital heart disease (CHD), 4 associated DCM, screened by single-strand DNA conformation polymorphism...
Sudden unexplained nocturnal death syndrome (SUNDS), a disorder found in southeast Asia, is characterized by an abnormal electrocardiogram with ST-segment elevation leads V1–V3 and sudden due to ventricular fibrillation, identical that seen Brugada syndrome. We screened patients SUNDS for mutations SCN5A, the gene known cause syndrome, as well genes encoding ion channels associated long-QT Ten families were enrolled, using single-strand DNA conformation polymorphism analysis, denaturing...
Recent studies have suggested that the etiology of autism spectrum disorder (ASD) may be caused by immunological factors, particularly abnormalities in innate immune system. However, it is still unclear which specific cytokines most importance. The current study therefore investigated showed altered concentrations blood ASD compared with healthy control children and were also correlated symptom severity. Our sample included 32 diagnosed 28 age sex-matched typically developing children....
A simple paper fluidic device provides a visual indication of blood coagulation status.
X-linked dilated cardiomyopathy (XLCM) has previously been shown to be due mutations in the dystrophin gene, which is located at Xp21. Mutations 5' portion of including muscle promoter, exon 1, and 1-intron 1 splice site, have reported previously. The purpose this study was analyze originally described family with XLCM (and other) for mutations.Polymerase chain reaction (PCR) used amplify genomic DNA, reverse-transcriptase PCR amplified cDNA from RNA obtained heart lymphoblastoid cell lines....
Loss of the NF1 tumor suppressor gene causes autosomal dominant condition, neurofibromatosis type 1 (NF1). Children and adults with suffer from pathologies including benign malignant tumors to cognitive deficits, seizures, growth abnormalities, peripheral neuropathies. encodes neurofibromin, a Ras-GTPase activating protein, mutations result in hyperactivated Ras signaling patients. Existing mutant mice mimic individual aspects NF1, but none comprehensively models disease. We describe...
To assess the value of combined Monocyte Distribution Width (MDW) and Procalcitonin (PCT) detection in diagnosing predicting neonatal sepsis outcomes. This retrospective study, conducted from January 2022 to December 2023.A analysis 39 30 non-infectious systemic inflammatory response syndrome (SIRS) cases was conducted. MDW, PCT, CRP levels were compared. Relationships between variables analyzed with Pearson correlation Cox regression models; diagnostic performance assessed using ROC curves....
Objectives To describe a case of Progressive Encephalopathy with or without Lipodystrophy (PELD), characterized by late onset neurological regression at 9 years old, due to homozygous c.974dupG variant in the BSCL2 gene. Methods An 11-year, 9-month-old girl repeated seizures over 2 underwent clinical assessment and genetic investigation. We also reviewed published literature. Results The patient exhibited mild intellectual disability, lipodystrophic appearance, precocious puberty, voracious...
Objective: To investigate whether additional early physiotherapy after stroke improved functional recovery in patients. Design: A prospective, randomized, controlled study. Setting: One ward and an acute unit a large teaching hospital, southern China. Subjects: Patients with first-onset consecutively admitted to the centre. Interventions: group ( n = 78) received (AEP) for 45 minutes, five days week four weeks starting within first since onset; routine therapy (RT) no professional...
Primary small cell carcinoma of esophagus (SCCE) is a relatively rare and highly aggressive tumor characterized by early dissemination poor prognosis. The optimal treatment has not yet been established, the role surgery remained controversial. Most limited diseases were treated conventionally surgery, but five-year survival rate was still very low. This retrospective study designed to investigate clinical characteristics, treatment, prognostic factors disease SCCE. Clinical data 40 SCCE...
In this study, the first group of single-stranded DNA aptamers that are highly specific to enterotoxigenic Escherichia coli (ETEC) K88 was obtained from an enriched oligonucleotide pool by SELEX (Systematic Evolution Ligands Exponential Enrichment) procedure, during which fimbriae protein used as target and bovine serum albumin counter targets. These were applied successfully in detection ETEC K88. They then grouped under different families based on similarity their secondary structure...
Epidemiological evidence suggests that formaldehyde (FA) exposure may influence the prevalence and severity of allergic asthma. However, role genetic background in FA-induced asthma-like responses is poorly understood. In present study, we investigated nature triggered by to different doses FA together with or without ovalbumin (OVA) two genetically mouse strains-BALB/c C57BL/6. Both strains were divided into main groups: non-sensitized group OVA-sensitized group. All groups exposed 0, 0.5...
We have devised a novel isothermal amplification technology, termed endonuclease restriction-mediated real-time multiple cross displacement (ET-MCDA), which facilitated multiplex, rapid, specific and sensitive detection of nucleic-acid sequences at constant temperature. The ET-MCDA integrated strategy, restriction cleavage fluorescence technique. In the system, functional primer E-CP1 or E-CP2 was constructed by adding short sequence 5' end CP1 CP2, respectively, new labeled with fluorophore...
The “no reaction” lateral flow assay (nrLFA) uses a simplified LFA structure with no conjugate pad and stored reagents. In the nrLFA, capillary-based transport time or distance is key indicator, rather than outcome of biochemical reaction. Hence, calibration reproducibility nrLFA device are critical. capillary properties several membrane types (nitrocellulose, nylon, cellulose acetate, polyethersulfone, polyvinylidene difluoride) evaluated. Flow rate evaluations MilliporeSigma Hi-Flow™ Plus...
The ketogenic diet (KD) has shown excellent performance in the treatment of refractory epilepsy, but how it works is not yet fully understood. Gut microbiota associated with various neurological disorders through brain-gut axis. Different dietary patterns have different effects on composition and function gut microbiota. Here, by analyzing fecal samples from some patients mitochondrial epilepsy before after KD 16SrRNA sequencing, we found that intervention reduced abundance Firmicutes...
Tuberous sclerosis complex (TSC) is a rare disease with high risk of epilepsy and cognitive impairment in children. Ketogenic diet (KD) therapy has been consistently reported to be beneficial TSC patients. In this study, we aimed investigate the efficacy safety KD treatment drug-resistant children TSC.In multicenter 53 (33 males 20 females) or caused by were retrospectively recruited from 10 hospitals January 1, 2010, December 31, 2020. Intention-to-treat analysis was used evaluate seizure...
Despite improvements in management, infective endocarditis (IE) is still associated with high mortality and morbidity. The outcome of patients IE remains unclear high-altitude areas China. To characterize the epidemiological features surgical outcomes, a retrospective analysis was conducted to 221 diagnosed from single center. In addition, assess prognosis patients, multivariate logistic regression model performed analyze affecting risk factors.