A5066862228- CRISPR and Genetic Engineering
- Food composition and properties
- Software-Defined Networks and 5G
- RNA regulation and disease
- Caching and Content Delivery
- Virus-based gene therapy research
- Polysaccharides Composition and Applications
- RNA and protein synthesis mechanisms
- Microbial Metabolites in Food Biotechnology
- Satellite Communication Systems
- IoT and Edge/Fog Computing
- Distributed and Parallel Computing Systems
- GABA and Rice Research
- Enzyme Production and Characterization
- Animal Genetics and Reproduction
- Network Security and Intrusion Detection
- Food Quality and Safety Studies
- Service-Oriented Architecture and Web Services
- RNA Interference and Gene Delivery
- Software System Performance and Reliability
- Solar Radiation and Photovoltaics
- Biofuel production and bioconversion
- Pluripotent Stem Cells Research
- Insect symbiosis and bacterial influences
- Opportunistic and Delay-Tolerant Networks
ShanghaiTech University
2018-2025
Henan Institute of Science and Technology
2009-2025
Shanghai Institute of Pharmaceutical Industry
2025
Shenzhen Technology University
2025
Zhejiang Lab
2025
Central Hospital of Zibo
2022-2024
Peking University
2024
China Tobacco
2023
University of Electro-Communications
2023
Wuhan University
2023
Abstract A recently developed adenine base editor (ABE) efficiently converts to G and is potentially useful for clinical applications. However, its precision efficiency in vivo remains be addressed. Here we achieve A-to-G conversion at frequencies up 100% by microinjection of ABE mRNA together with sgRNAs. We then generate mouse models harboring clinically relevant mutations Ar Hoxd13 , which recapitulates respective defects. Furthermore, both C-to-T editing using a combination SaBE3, thus...
The gut microbiota has been linked to cardiovascular diseases. However, the composition and functional capacity of microbiome in relation diseases have not systematically examined. Here, we perform a metagenome-wide association study on stools from 218 individuals with atherosclerotic disease (ACVD) 187 healthy controls. ACVD deviates status by increased abundance Enterobacteriaceae Streptococcus spp. and, functionally, potential for metabolism or transport several molecules important...
Three starches from the wheat varieties AK58, ZM18 and YZ4110 were separated into large (A) small (B) granules, which characterized structurally evaluated for their functional properties. SEM results showed that size of A-granules about same, but sizes B-granules AK58 larger than those YZ4110. FTIR spectra all samples exhibited a similar pattern, with seven main modes maximum absorbance peaks near 3,500, 3,000, 1,600, 1,400, 1,000, 800, 500 cm−1. The had less amylose content, although...
Abstract Coronary heart disease (CHD) is top risk factor for health in modern society, causing high mortality rate each year. However, there no reliable way early diagnosis and prevention of CHD so far. So study the mechanism development novel biomarkers urgently needed. In this study, metabolomics metagenomics technology are applied to discover new from plasma urine 59 patients 43 healthy controls trace their origin. We identify GlcNAc-6-P which has good diagnostic capability can be used as...
There are urgent demands for efficient treatment of heritable genetic diseases. The base editing technology has displayed its efficiency and precision in substitution human embryos, providing a potential early-stage Taking advantage this technology, we corrected Marfan syndrome pathogenic mutation, FBN1T7498C. We first tested the feasibility mutant cells, then successfully achieved correction heterozygous embryos. results showed that BE3 mediated perfect at about 89%. Importantly, no...
Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model development livestock breeding. Despite ongoing attempts since the 1990s, no stably defined pig cell line has been established. Here, guided by insights from a large-scale single-cell transcriptome analysis of embryos embryonic day (E) 0 E14, specifically, tracing pluripotency changes during epiblast development, we developed an in vitro culture medium...
Introduction Cervical cancer is the fourth most commonly diagnosed malignancy among women globally, with HPV infection being primary cause. Large-scale screening crucial for early detection, as appropriate intervention in HPV-positive individuals can significantly reduce cervical incidence and mortality. Methods Data were extracted from Shenzhen Maternal Child Health Management Information System, covering 18,667 instances 15,850 January 1, 2020, to December 31, 2023. prevalence...
luteolin and phospholipid complex was prepared to improve the lipophilic properties of luteolin. The physicochemical were analyzed by ultraviolet-visible spectrometry (UV), infrared (IR), X-ray diffractometry (XRD) differential scanning calorimetry (DSC). results showed that in joined non-covalent-bonds did not form a new compound. It found an effective scavenger DPPH radicals, with IC50 value 28.33 μg/mL. In Rancimat antioxidant test using lard oil as substrate, also strong activity.
Designing efficient and specific CRISPR single-guide RNAs (sgRNAs) is vital for the successful application of technology.Currently, a growing number new RNA-guided endonucleases with different protospacer adjacent motif (PAM) have been discovered, suggesting necessity to develop versatile tool designing sgRNA meet requirement DNA endonucleases.Here, we report development flexible design program named "CRISPR-offinder".Support user-defined PAM length was provided increase targeting range...
Traditional CRISPR/Cas9-based gene knockouts are generated by introducing DNA double-strand breaks (DSBs), but this may cause excessive damage or cell death. CRISPR-based cytosine base editors (CBEs) and adenine (ABEs) can facilitate C-to-T A-to-G exchanges, respectively, without DSBs. CBEs have been employed in a knockout strategy: CRISPR-STOP i-STOP changes single nucleotides to induce in-frame stop codons. However, strategy is not applicable for some genes, the unwanted mutations CBE...
With massive sorts of terminals, devices, and machines connecting to 5G, a tremendous surge data makes cyber-security pressing issue, conventional countermeasures are facing unprecedented challenges. Recently, with the rise ML (Machine Learning) SDN/NFV-based (Software-Defined Networks/Network Functions Virtualization) SFC (Service Function Chaining) techniques, how leverage them for security enhancement in MEC (Multi-access/Mobile Edge Computing) has received much attention. Hence, this...
Software-Defined Networking (SDN) and Network Functions Virtualization (NFV) technologies facilitate deploying Service Function Chains (SFCs) at clouds in efficiency flexibility. However, it is still challenging to efficiently chain Virtualized (VNFs) overlay networks without knowledge of underlying network configurations. Although there are many deterministic approaches for VNF placement chaining, they have high complexity depend on state information substrate networks. Fortunately,...
RNA splicing is related to many human diseases; however, lack of efficient genetic approaches modulate has prevented us from dissecting their functions in diseases. Recently developed base editors (BEs) offer a new strategy by converting conservative splice sites, but it limited the editing precision and scope. To overcome limitations currently available BE-based tools, we combined SpCas9-NG with ABEmax generate BE, ABEmax-NG. We demonstrated that ABEmax-NG performed precise A⋅T G⋅C...