A5100460763- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Plant Virus Research Studies
- RNA regulation and disease
- Fungal and yeast genetics research
- Wind and Air Flow Studies
- Biosensors and Analytical Detection
- Aerodynamics and Acoustics in Jet Flows
- Advanced Adaptive Filtering Techniques
- Retinal Development and Disorders
- Bacterial Genetics and Biotechnology
- Innovation and Socioeconomic Development
- GABA and Rice Research
- Protein Degradation and Inhibitors
- Extracellular vesicles in disease
- Microbial Natural Products and Biosynthesis
- Economic and Technological Innovation
- Insect symbiosis and bacterial influences
- RNA Research and Splicing
- Probiotics and Fermented Foods
- Cancer Genomics and Diagnostics
- bioluminescence and chemiluminescence research
Institute of Animal Sciences
2022-2025
State Key Laboratory of Animal Nutrition
2025
Chinese Academy of Agricultural Sciences
2020-2024
Zhejiang Lab
2024
University of Shanghai for Science and Technology
2009-2024
Center for Life Sciences
2024
Guangdong Pharmaceutical University
2024
Anhui Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2020-2022
Xiamen University
2022
Summary CRISPR/Cas‐base editing is an emerging technology that could convert a nucleotide to another type at the target site. In this study, A3A‐PBE system consisting of human A3A cytidine deaminase fused with Cas9 nickase and uracil glycosylase inhibitor was established developed in allotetraploid Brassica napus . We designed three sgRNAs ALS , RGA IAA7 genes, respectively. Base‐editing efficiency demonstrated be more than 20% for all genes. Target sequencing results revealed window ranged...
Comprehensive discovery of genetic mechanisms drug resistance and identification in vivo targets represent significant challenges. Here we present a functional variomics technology the model organism Saccharomyces cerevisiae. This tool analyzes numerous variants effectively tackles both problems simultaneously. Using this tool, discovered almost all genes that, due to mutations or modest overexpression, confer rapamycin, cycloheximide, amphotericin B. Most among were targets, including...
Small mutations in the core promoter region of a gene may result substantial changes expression strengths. However, targeting TA-rich sequences promoters pose challenge for Cas9 variants such as SpCas9 and other G-rich PAM-compatible Cas9s. In this study, we engineered unique FrCas9 system derived from
The A-to-G mutation (FecB) in the BMPR1B gene is strongly linked to fertility sheep, significantly increasing ovulation rates and litter sizes compared wild-type populations. rapid reliable screening of FecB therefore critical for advancing sheep breeding programs. This study aimed develop a fast accurate method detecting genotyping enhance reproduction productivity. To achieve this, we integrated CRISPR-Cas12a system with an optimized amplification refractory (ARMS). A similar DNA origami...
The diploid Brassica oleracea and allopolyploidy napus are predominant members of commonly consumed vegetables plant oil, respectively. B. mainly include Broccoli, Cauliflower, Cabbage, Brussels sprouts, Kohlrabi. complex genome structure gene function redundancy the main obstacles for stacking through traditional cross-breeding approach. To solve this problem, high-efficiency CRISPR/Cas9 genome-editing technologies have been established (Li et al., 2018). However, an open question is that...
The cashmere goat breed is known to provide excellent quality cashmere.Here, we attempted high-yielding goats by specifically inserting the Tβ4 gene into CCR5 locus and provided an animal model for future research.We successfully obtained knock-in without any screening fluorescent markers using CRISPR/Cas9 technology.A series of experiments were performed examine physical conditions characteristics goat.The exhibited increase in yield 74.5% affecting fineness quality.Additionally, RNA-seq...
Lactococcus cremoris (homotypic synonym: lactis) is receiving increasing attention as a prominent vehicle for the delivery of live vaccines. This can hardly be achieved without developing tools genetic manipulation L. cremoris, and paucity studies on endogenous promoters has attracted our attention. Here, we report discovery characterization 29 candidate identified from subsp. NZ9000 by RNA sequencing analysis. Furthermore, 18 possible constitutive were obtained RT-qPCR screening these...
Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation novel tasks. Transcriptional gene regulation systems induced by small molecules prokaryotes provide a rich source logic gates. Cross-regulation, whereby promoter is activated different or promoters are one molecule, can be used to design an OR-gate achieve cross-talk between networks cells. Acinetobacter baylyi ADP1 naturally transformable, readily editing its chromosomal...
The two essential editing elements in the clustered regularly interspaced short palindromic repeats (CRISPR) system are promoter and single-guide RNA (sgRNA), latter of which determines whether Cas protein can precisely target a specific location to edit targeted gene. Therefore, selection sgRNA is crucial efficiency CRISPR system. Various online prediction tools for currently available. These predict all possible sgRNAs gene rank according certain scoring criteria demands user.We designed...
The echo signal polluted by noise needs to be filtered in the ultrasound imaging system. However, conventional fixed bandpass filter doesn't work effectively, because bandwidth and center frequency of is time-varying. Adaptive with NLMS then applied resolve this problem. A method evaluate its performance also developed. Phantom experiment shows that adaptive able enhance SER (Signal Error Ratio) for about 8dB.
SUMMARY The genome of an organism is inherited from its ancestor and keeps evolving over time, however, how much the current version could be altered remains unknown. Here, we use left arm chromosome XII ( chrXIIL ) as example to probe plasticity in Saccharomyces cerevisiae . A neochromosome was designed harbor originally dispersed genes. essentiality sequences dissected by targeted DNA removal, truncation random deletion. Notably, 12 genes were sufficient for survival, while 25 are required...
Telomerase is a well-known cancer biomarker, and the reliable sensitive detection of telomerase activity vital significance for diagnosis treatment. Despite many attempts, methods assay mainly depend on various nucleic acid amplification, which requires complex primers/probes design multiple enzymes. In addition, experimental procedures may introduce false-positive results from cross-contamination amplification products challenging reliability telomerase-based diagnostics detection. Herein,...